AT18H_ARATH
ID AT18H_ARATH Reviewed; 927 AA.
AC Q8H1Q5; Q9C5I7; Q9ZVM1;
DT 03-APR-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=Autophagy-related protein 18h;
DE Short=AtATG18h;
GN Name=ATG18H; OrderedLocusNames=At1g54710; ORFNames=T22H22.14;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP GENE FAMILY, INDUCTION, AND TISSUE SPECIFICITY.
RX PubMed=15860012; DOI=10.1111/j.1365-313x.2005.02397.x;
RA Xiong Y., Contento A.L., Bassham D.C.;
RT "AtATG18a is required for the formation of autophagosomes during nutrient
RT stress and senescence in Arabidopsis thaliana.";
RL Plant J. 42:535-546(2005).
CC -!- FUNCTION: The PI(3,5)P2 regulatory complex regulates both the synthesis
CC and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2).
CC Required for autophagy (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the PI(3,5)P2 regulatory complex at least
CC composed of ATG18, SAC/FIG4, FAB1 and VAC14. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Preautophagosomal structure membrane
CC {ECO:0000250}; Peripheral membrane protein {ECO:0000250}. Vacuole
CC membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}.
CC Note=Peripheral membrane protein of pre-autophagosomal structure (PAS)
CC and vacuole. {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in roots, flowers and leaves.
CC {ECO:0000269|PubMed:15860012}.
CC -!- INDUCTION: By sucrose and nitrogen starvation.
CC {ECO:0000269|PubMed:15860012}.
CC -!- DOMAIN: The first protein part may form a beta-propeller domain
CC involved in specific binding to phosphatidylinositol 3,5-bisphosphate
CC (PIP2), leading to the association of the protein to the membrane.
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the WD repeat PROPPIN family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC64884.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC005388; AAC64884.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE33137.1; -; Genomic_DNA.
DR EMBL; AF360222; AAK25932.1; -; mRNA.
DR EMBL; AY142626; AAN13084.1; -; mRNA.
DR PIR; C96589; C96589.
DR RefSeq; NP_564664.1; NM_104346.3.
DR AlphaFoldDB; Q8H1Q5; -.
DR SMR; Q8H1Q5; -.
DR STRING; 3702.AT1G54710.1; -.
DR iPTMnet; Q8H1Q5; -.
DR PaxDb; Q8H1Q5; -.
DR PRIDE; Q8H1Q5; -.
DR ProteomicsDB; 246524; -.
DR EnsemblPlants; AT1G54710.1; AT1G54710.1; AT1G54710.
DR GeneID; 841912; -.
DR Gramene; AT1G54710.1; AT1G54710.1; AT1G54710.
DR KEGG; ath:AT1G54710; -.
DR Araport; AT1G54710; -.
DR TAIR; locus:2199512; AT1G54710.
DR eggNOG; KOG2109; Eukaryota.
DR HOGENOM; CLU_003829_1_0_1; -.
DR InParanoid; Q8H1Q5; -.
DR OMA; EPLQWWD; -.
DR OrthoDB; 537714at2759; -.
DR PhylomeDB; Q8H1Q5; -.
DR PRO; PR:Q8H1Q5; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q8H1Q5; baseline and differential.
DR Genevisible; Q8H1Q5; AT.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0034045; C:phagophore assembly site membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005774; C:vacuolar membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0006914; P:autophagy; IEA:UniProtKB-KW.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0042594; P:response to starvation; IEP:TAIR.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR022175; BCAS3.
DR InterPro; IPR045142; BCAS3-like.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR PANTHER; PTHR13268; PTHR13268; 1.
DR Pfam; PF12490; BCAS3; 1.
DR Pfam; PF00400; WD40; 1.
DR SMART; SM00320; WD40; 3.
DR SUPFAM; SSF50978; SSF50978; 1.
PE 2: Evidence at transcript level;
KW Autophagy; Membrane; Protein transport; Reference proteome; Repeat;
KW Transport; Vacuole; WD repeat.
FT CHAIN 1..927
FT /note="Autophagy-related protein 18h"
FT /id="PRO_0000421886"
FT REPEAT 379..419
FT /note="WD 1"
FT REPEAT 441..482
FT /note="WD 2"
FT REGION 1..25
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 333..353
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 750..788
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 844..927
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 750..770
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 844..865
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 892..906
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 847
FT /note="S -> N (in Ref. 3; AAK25932)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 927 AA; 100514 MW; 2EE0C5DDEC3BA5CF CRC64;
MKSNSKVNIN NNGDNHNQTK NNGTNGFLPN SLKFISTCIR TASSGVRSAS ASVAASLSSD
SHELKDQVLW SSFDRLHTSE SSFKNVLLLG YTNGFQVLDI DDSNDVTEFV SRRDDPVTFL
QMQPLPAKCD GVEGFRSSHP ILLAVADEAK GSGPIVTSRD GSVRNGYEDP LALSPTVVRF
YSLRSHNYVH VLRFRSTVYM VRCSPRIVAV GLGSQIYCFD ALTLENKFSV LSYPVPQLGN
QGISGVNVGY GPMAVGARWL AYASNSPLSS SIGRLSPQNV TPPGVSPSTS PNNGNLVARY
AMESSKHLAA GLLNLGDKGY KTISKYCQDL KHDGPGPSLS SSPGRKVGRV GSHSAESDVV
GTVIVKDFES RAIIAQFRAH TSPISALCFD PSGTLLVTAS IHGNNINVFR IMPTPTKNGP
GAQSYDWSSS HVPLYKLHRG MTSAVIQDIC FSSYSQWIAI VSSKSTCHIY VLSPFGGENV
LEIRNSQFDG PTLAPTLSLP WWSSPSFMTT HFSYPPPASV TLSVVSRIKC NNFFHAASSV
VGKPTFPSGC LAAVFHQSVP QESQSSSPAL DYLLVYTPSG HVVQYKLIPS LGGDQAESNT
RNGATSGLTS EEELRVKVEP VQCWDVCRRA DWPEREENIC GLTYGGRKNA ELTVDTSDSE
DQTKPLEKHH VYLANAEVLI NSGRKPIWQN SEISFYPMYP PDSDGKNLNS HQGGGETEIG
KVSANEVDIR RKDLLPVYDN FHSVYTSMRN RGFSGERDSD SSSSSDPGQV KEMHPFNGMV
YPEDEERRGS AHFALTPNQN PHTGIVTFKQ PVVSISSAVK DTDYIDDDAH VLPKNASLPA
ETKIENSSGI SGDSNVSSNR SDMSMNAADE GEGPIDGSPN FEQFFEEVVS NETVTETEHK
DAPSDGKLDD DEDDDMLGGV FAFSEEG
