PMT8_ARATH
ID PMT8_ARATH Reviewed; 623 AA.
AC Q940J9; P93817; Q56WM9; Q8LG52; Q94EZ3;
DT 23-MAR-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=Probable methyltransferase PMT8;
DE EC=2.1.1.-;
GN OrderedLocusNames=At1g04430; ORFNames=F19P19.11;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 381-623.
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP GENE FAMILY.
RX PubMed=17461780; DOI=10.1111/j.1365-313x.2007.03123.x;
RA Krupkova E., Immerzeel P., Pauly M., Schmulling T.;
RT "The TUMOROUS SHOOT DEVELOPMENT2 gene of Arabidopsis encoding a putative
RT methyltransferase is required for cell adhesion and co-ordinated plant
RT development.";
RL Plant J. 50:735-750(2007).
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000305}; Single-
CC pass type II membrane protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the methyltransferase superfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB70432.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAM61029.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AC000104; AAB70432.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE27696.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE27697.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM58680.1; -; Genomic_DNA.
DR EMBL; AY054530; AAK96721.1; -; mRNA.
DR EMBL; AY064624; AAL47337.1; -; mRNA.
DR EMBL; AF387011; AAK62456.1; -; mRNA.
DR EMBL; AY081460; AAM10022.1; -; mRNA.
DR EMBL; AY084457; AAM61029.1; ALT_INIT; mRNA.
DR EMBL; AK222007; BAD94636.1; -; mRNA.
DR PIR; D86176; D86176.
DR RefSeq; NP_001117225.1; NM_001123753.2.
DR RefSeq; NP_001321096.1; NM_001331478.1.
DR RefSeq; NP_563706.1; NM_100323.7.
DR AlphaFoldDB; Q940J9; -.
DR SMR; Q940J9; -.
DR BioGRID; 24757; 2.
DR IntAct; Q940J9; 1.
DR STRING; 3702.AT1G04430.1; -.
DR PaxDb; Q940J9; -.
DR PRIDE; Q940J9; -.
DR ProteomicsDB; 236649; -.
DR EnsemblPlants; AT1G04430.1; AT1G04430.1; AT1G04430.
DR EnsemblPlants; AT1G04430.2; AT1G04430.2; AT1G04430.
DR EnsemblPlants; AT1G04430.3; AT1G04430.3; AT1G04430.
DR GeneID; 839522; -.
DR Gramene; AT1G04430.1; AT1G04430.1; AT1G04430.
DR Gramene; AT1G04430.2; AT1G04430.2; AT1G04430.
DR Gramene; AT1G04430.3; AT1G04430.3; AT1G04430.
DR KEGG; ath:AT1G04430; -.
DR Araport; AT1G04430; -.
DR TAIR; locus:2018329; AT1G04430.
DR eggNOG; ENOG502QTWS; Eukaryota.
DR HOGENOM; CLU_010485_2_2_1; -.
DR InParanoid; Q940J9; -.
DR OMA; VEHYWNI; -.
DR OrthoDB; 288023at2759; -.
DR PhylomeDB; Q940J9; -.
DR PRO; PR:Q940J9; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q940J9; baseline and differential.
DR Genevisible; Q940J9; AT.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005768; C:endosome; HDA:TAIR.
DR GO; GO:0005794; C:Golgi apparatus; HDA:TAIR.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0000138; C:Golgi trans cisterna; HDA:TAIR.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0000325; C:plant-type vacuole; HDA:TAIR.
DR GO; GO:0005802; C:trans-Golgi network; HDA:TAIR.
DR GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.150; -; 1.
DR InterPro; IPR004159; Put_SAM_MeTrfase.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR10108; PTHR10108; 1.
DR Pfam; PF03141; Methyltransf_29; 1.
DR SUPFAM; SSF53335; SSF53335; 2.
PE 2: Evidence at transcript level;
KW Glycoprotein; Golgi apparatus; Membrane; Methyltransferase;
KW Reference proteome; Signal-anchor; Transferase; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..623
FT /note="Probable methyltransferase PMT8"
FT /id="PRO_0000393248"
FT TOPO_DOM 1..13
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 14..34
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 35..623
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT CARBOHYD 204
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 350
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 588
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 190
FT /note="Y -> C (in Ref. 3; AAM10022/AAK62456)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 623 AA; 70508 MW; 048A3BA631402954 CRC64;
MMRGRSDGGL KKRLIASVCV VALFVCFLFM YYGSSSQGAS ALEYGRSLRK LGSSYLSGDD
DNGDTKQDDS VANAEDSLVV AKSFPVCDDR HSEIIPCLDR NFIYQMRLKL DLSLMEHYER
HCPPPERRFN CLIPPPSGYK VPIKWPKSRD EVWKANIPHT HLAKEKSDQN WMVEKGEKIS
FPGGGTHFHY GADKYIASIA NMLNFSNDVL NDEGRLRTVL DVGCGVASFG AYLLASDIMT
MSLAPNDVHQ NQIQFALERG IPAYLGVLGT KRLPYPSRSF EFAHCSRCRI DWLQRDGLLL
LELDRVLRPG GYFAYSSPEA YAQDEENLKI WKEMSALVER MCWRIAVKRN QTVVWQKPLS
NDCYLEREPG TQPPLCRSDA DPDAVAGVSM EACITPYSKH DHKTKGSGLA PWPARLTSSP
PRLADFGYST DMFEKDTELW KQQVDSYWNL MSSKVKSNTV RNIMDMKAHM GSFAAALKDK
DVWVMNVVSP DGPNTLKLIY DRGLIGTNHN WCEAFSTYPR TYDLLHAWSI FSDIKSKGCS
AEDLLIEMDR ILRPTGFVII RDKQSVVESI KKYLQALHWE TVASEKVNTS SELDQDSEDG
ENNVVFIVQK KLWLTSESLR DSE
