ID   PMTC_ARATH              Reviewed;         682 AA.
AC   Q9FG39;
DT   23-MAR-2010, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 112.
DE   RecName: Full=Probable methyltransferase PMT12;
DE            EC=2.1.1.-;
GN   OrderedLocusNames=At5g06050; ORFNames=K18J17.25;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RA   Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H.,
RA   Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 5. XI.";
RL   Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10470850; DOI=10.1093/dnares/6.3.183;
RA   Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Kotani H.,
RA   Miyajima N., Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 5. IX. Sequence
RT   features of the regions of 1,011,550 bp covered by seventeen P1 and TAC
RT   clones.";
RL   DNA Res. 6:183-195(1999).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA   Cheuk R., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT   "Arabidopsis ORF clones.";
RL   Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA   Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA   Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA   Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA   Shinozaki K.;
RT   "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL   Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN   [6]
RP   GENE FAMILY.
RX   PubMed=17461780; DOI=10.1111/j.1365-313x.2007.03123.x;
RA   Krupkova E., Immerzeel P., Pauly M., Schmulling T.;
RT   "The TUMOROUS SHOOT DEVELOPMENT2 gene of Arabidopsis encoding a putative
RT   methyltransferase is required for cell adhesion and co-ordinated plant
RT   development.";
RL   Plant J. 50:735-750(2007).
CC   -!- SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000250}; Single-
CC       pass type II membrane protein.
CC   -!- SIMILARITY: Belongs to the methyltransferase superfamily.
CC       {ECO:0000305}.
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DR   EMBL; AP002030; BAB10206.1; -; Genomic_DNA.
DR   EMBL; AB017060; BAB10206.1; JOINED; Genomic_DNA.
DR   EMBL; CP002688; AED90959.1; -; Genomic_DNA.
DR   EMBL; BT015368; AAU05491.1; -; mRNA.
DR   EMBL; BT020595; AAW80868.1; -; mRNA.
DR   EMBL; AK226215; BAE98380.1; -; mRNA.
DR   RefSeq; NP_196224.1; NM_120687.5.
DR   AlphaFoldDB; Q9FG39; -.
DR   STRING; 3702.AT5G06050.1; -.
DR   PaxDb; Q9FG39; -.
DR   PRIDE; Q9FG39; -.
DR   ProteomicsDB; 226188; -.
DR   EnsemblPlants; AT5G06050.1; AT5G06050.1; AT5G06050.
DR   GeneID; 830492; -.
DR   Gramene; AT5G06050.1; AT5G06050.1; AT5G06050.
DR   KEGG; ath:AT5G06050; -.
DR   Araport; AT5G06050; -.
DR   TAIR; locus:2153704; AT5G06050.
DR   eggNOG; ENOG502QQFS; Eukaryota.
DR   HOGENOM; CLU_010485_2_4_1; -.
DR   InParanoid; Q9FG39; -.
DR   OMA; ISFGNHT; -.
DR   OrthoDB; 366627at2759; -.
DR   PhylomeDB; Q9FG39; -.
DR   PRO; PR:Q9FG39; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q9FG39; baseline and differential.
DR   Genevisible; Q9FG39; AT.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR004159; Put_SAM_MeTrfase.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   PANTHER; PTHR10108; PTHR10108; 1.
DR   Pfam; PF03141; Methyltransf_29; 1.
DR   SUPFAM; SSF53335; SSF53335; 2.
PE   2: Evidence at transcript level;
KW   Glycoprotein; Golgi apparatus; Membrane; Methyltransferase;
KW   Reference proteome; Signal-anchor; Transferase; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..682
FT                   /note="Probable methyltransferase PMT12"
FT                   /id="PRO_0000393252"
FT   TOPO_DOM        1..11
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        12..32
FT                   /note="Helical; Signal-anchor for type II membrane protein"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        33..682
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        67
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        103
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        125
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        155
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        173
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        193
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        273
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        350
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        395
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        419
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        600
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        625
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   682 AA;  77711 MW;  5DF683F245302BE7 CRC64;
     MKLFLNSNLL RNSIFFKISA FVLISVACFF LGKHWSEDGF RRLIFFSAEP SRSPIVALSP
     DFGKTYNISG LIYESHPILP PSLSPPPPPD SVELKVFGIV NENGTMSDEF QIGDYDVESA
     ETLGNQTEFE SSDDDDIKST TARVSVRKFE ICSENMTEYI PCLDNVEAIK RLNSTARGER
     FERNCPNDGM GLNCTVPIPQ GYRSPIPWPR SRDEVWFNNV PHTKLVEDKG GQNWIYKEND
     KFKFPGGGTQ FIHGADQYLD QISQMIPDIS FGNHTRVVLD IGCGVASFGA YLMSRNVLTM
     SIAPKDVHEN QIQFALERGV PAMVAAFTTR RLLYPSQAFD LVHCSRCRIN WTRDDGILLL
     EVNRMLRAGG YFVWAAQPVY KHEKALEEQW EEMLNLTTRL CWVLVKKEGY IAIWQKPVNN
     TCYLSRGAGV SPPLCNSEDD PDNVWYVDLK ACITRIEENG YGANLAPWPA RLLTPPDRLQ
     TIQIDSYIAR KELFVAESKY WKEIISNYVN ALHWKQIGLR NVLDMRAGFG GFAAALAELK
     VDCWVLNVIP VSGPNTLPVI YDRGLLGVMH DWCEPFDTYP RTYDLLHAAG LFSIERKRCN
     MTTMMLEMDR ILRPGGRVYI RDTINVTSEL QEIGNAMRWH TSLRETAEGP HSSYRVLLCE
     KRFESSEKRR TKKRRKTKGK RA