PMTI_ARATH
ID PMTI_ARATH Reviewed; 639 AA.
AC Q9C884; Q9LP29;
DT 23-MAR-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=Probable methyltransferase PMT18;
DE EC=2.1.1.-;
GN OrderedLocusNames=At1g33170; ORFNames=T16O9.7, T9L6.6;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP GENE FAMILY.
RX PubMed=17461780; DOI=10.1111/j.1365-313x.2007.03123.x;
RA Krupkova E., Immerzeel P., Pauly M., Schmulling T.;
RT "The TUMOROUS SHOOT DEVELOPMENT2 gene of Arabidopsis encoding a putative
RT methyltransferase is required for cell adhesion and co-ordinated plant
RT development.";
RL Plant J. 50:735-750(2007).
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000305};
CC Single-pass type II membrane protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the methyltransferase superfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF97349.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC021045; AAF97349.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AC027035; AAG51278.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE31570.1; -; Genomic_DNA.
DR EMBL; AY140083; AAM98224.1; -; mRNA.
DR EMBL; BT009665; AAP78933.1; -; mRNA.
DR PIR; G86455; G86455.
DR RefSeq; NP_564419.1; NM_103049.3.
DR AlphaFoldDB; Q9C884; -.
DR IntAct; Q9C884; 1.
DR STRING; 3702.AT1G33170.1; -.
DR PaxDb; Q9C884; -.
DR PRIDE; Q9C884; -.
DR ProteomicsDB; 226284; -.
DR EnsemblPlants; AT1G33170.1; AT1G33170.1; AT1G33170.
DR GeneID; 840213; -.
DR Gramene; AT1G33170.1; AT1G33170.1; AT1G33170.
DR KEGG; ath:AT1G33170; -.
DR Araport; AT1G33170; -.
DR TAIR; locus:2196651; AT1G33170.
DR eggNOG; ENOG502QRZJ; Eukaryota.
DR HOGENOM; CLU_010485_2_2_1; -.
DR InParanoid; Q9C884; -.
DR OMA; THYKGWE; -.
DR OrthoDB; 238716at2759; -.
DR PhylomeDB; Q9C884; -.
DR PRO; PR:Q9C884; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9C884; baseline and differential.
DR Genevisible; Q9C884; AT.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005634; C:nucleus; HDA:TAIR.
DR GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.150; -; 1.
DR InterPro; IPR004159; Put_SAM_MeTrfase.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR10108; PTHR10108; 1.
DR Pfam; PF03141; Methyltransf_29; 1.
DR SUPFAM; SSF53335; SSF53335; 2.
PE 2: Evidence at transcript level;
KW Endoplasmic reticulum; Glycoprotein; Membrane; Methyltransferase;
KW Reference proteome; Signal-anchor; Transferase; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..639
FT /note="Probable methyltransferase PMT18"
FT /id="PRO_0000393258"
FT TOPO_DOM 1..19
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 20..42
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 43..639
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT REGION 41..86
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 104
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 427
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 639 AA; 73186 MW; CC54BC37BA88AF07 CRC64;
MAKENSSHSL AEAKRKRLTW ILCVSGLCIL SYVLGSWQTN TVPTSSSEAY SRMGCDETST
TTRAQTTQTQ TNPSSDDTSS SLSSSEPVEL DFESHHKLEL KITNQTVKYF EPCDMSLSEY
TPCEDRERGR RFDRNMMKYR ERHCPSKDEL LYCLIPPPPN YKIPFKWPQS RDYAWYDNIP
HKELSIEKAI QNWIQVEGER FRFPGGGTMF PRGADAYIDD IARLIPLTDG AIRTAIDTGC
GVASFGAYLL KRDIVAMSFA PRDTHEAQVQ FALERGVPAI IGIMGSRRLP YPARAFDLAH
CSRCLIPWFQ NDGLYLTEVD RVLRPGGYWI LSGPPINWKK YWKGWERSQE DLKQEQDSIE
DAARSLCWKK VTEKGDLSIW QKPINHVECN KLKRVHKTPP LCSKSDLPDF AWYKDLESCV
TPLPEANSSD EFAGGALEDW PNRAFAVPPR IIGGTIPDIN AEKFREDNEV WKERISYYKQ
IMPELSRGRF RNIMDMNAYL GGFAAAMMKY PSWVMNVVPV DAEKQTLGVI FERGFIGTYQ
DWCEGFSTYP RTYDLIHAGG LFSIYENRCD VTLILLEMDR ILRPEGTVVF RDTVEMLTKI
QSITNGMRWK SRILDHERGP FNPEKILLAV KSYWTGPSS
