PMTK_ARATH
ID PMTK_ARATH Reviewed; 603 AA.
AC Q9C6S7; C0Z341; Q8H7B2;
DT 23-MAR-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Probable methyltransferase PMT20;
DE EC=2.1.1.-;
GN OrderedLocusNames=At1g31850; ORFNames=F5M6.14;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Stracke R., Palme K.;
RT "Signal peptide selection derived cDNAs from Arabidopsis thaliana leaves
RT and guard cells.";
RL Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=19423640; DOI=10.1093/dnares/dsp009;
RA Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M.,
RA Shinozaki K.;
RT "Analysis of multiple occurrences of alternative splicing events in
RT Arabidopsis thaliana using novel sequenced full-length cDNAs.";
RL DNA Res. 16:155-164(2009).
RN [6]
RP GENE FAMILY.
RX PubMed=17461780; DOI=10.1111/j.1365-313x.2007.03123.x;
RA Krupkova E., Immerzeel P., Pauly M., Schmulling T.;
RT "The TUMOROUS SHOOT DEVELOPMENT2 gene of Arabidopsis encoding a putative
RT methyltransferase is required for cell adhesion and co-ordinated plant
RT development.";
RL Plant J. 50:735-750(2007).
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000305}; Single-
CC pass type II membrane protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the methyltransferase superfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAN60317.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=BAH57120.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AF083759; AAN60317.1; ALT_FRAME; mRNA.
DR EMBL; AC079041; AAG50728.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE31405.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE31406.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE31407.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM58540.1; -; Genomic_DNA.
DR EMBL; AY128291; AAM91099.1; -; mRNA.
DR EMBL; BT000825; AAN33200.1; -; mRNA.
DR EMBL; AK319005; BAH57120.1; ALT_FRAME; mRNA.
DR PIR; F86442; F86442.
DR RefSeq; NP_001319125.1; NM_001332982.1.
DR RefSeq; NP_174468.1; NM_102922.5.
DR RefSeq; NP_849736.1; NM_179405.4.
DR RefSeq; NP_973949.1; NM_202220.2.
DR AlphaFoldDB; Q9C6S7; -.
DR STRING; 3702.AT1G31850.1; -.
DR PaxDb; Q9C6S7; -.
DR PRIDE; Q9C6S7; -.
DR ProteomicsDB; 226155; -.
DR EnsemblPlants; AT1G31850.1; AT1G31850.1; AT1G31850.
DR EnsemblPlants; AT1G31850.2; AT1G31850.2; AT1G31850.
DR EnsemblPlants; AT1G31850.3; AT1G31850.3; AT1G31850.
DR EnsemblPlants; AT1G31850.4; AT1G31850.4; AT1G31850.
DR GeneID; 840075; -.
DR Gramene; AT1G31850.1; AT1G31850.1; AT1G31850.
DR Gramene; AT1G31850.2; AT1G31850.2; AT1G31850.
DR Gramene; AT1G31850.3; AT1G31850.3; AT1G31850.
DR Gramene; AT1G31850.4; AT1G31850.4; AT1G31850.
DR KEGG; ath:AT1G31850; -.
DR Araport; AT1G31850; -.
DR TAIR; locus:2034522; AT1G31850.
DR eggNOG; ENOG502QQA9; Eukaryota.
DR HOGENOM; CLU_010485_2_2_1; -.
DR InParanoid; Q9C6S7; -.
DR OMA; KMRAQHY; -.
DR OrthoDB; 312524at2759; -.
DR PhylomeDB; Q9C6S7; -.
DR PRO; PR:Q9C6S7; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9C6S7; baseline and differential.
DR Genevisible; Q9C6S7; AT.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005768; C:endosome; HDA:TAIR.
DR GO; GO:0005794; C:Golgi apparatus; HDA:TAIR.
DR GO; GO:0005797; C:Golgi medial cisterna; HDA:TAIR.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005802; C:trans-Golgi network; HDA:TAIR.
DR GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.150; -; 1.
DR InterPro; IPR004159; Put_SAM_MeTrfase.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR10108; PTHR10108; 1.
DR Pfam; PF03141; Methyltransf_29; 1.
DR SUPFAM; SSF53335; SSF53335; 2.
PE 2: Evidence at transcript level;
KW Glycoprotein; Golgi apparatus; Membrane; Methyltransferase;
KW Reference proteome; Signal-anchor; Transferase; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..603
FT /note="Probable methyltransferase PMT20"
FT /id="PRO_0000393260"
FT TOPO_DOM 1..16
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 17..37
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 38..603
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT CARBOHYD 313
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 600
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 603 AA; 68797 MW; 39B0B7BED1EFD3A1 CRC64;
MKSGKQSSQP EKGTSRILSL TVLFIAFCGF SFYLGGIFCS ERDKIVAKDV TRTTTKAVAS
PKEPTATPIQ IKSVSFPECG SEFQDYTPCT DPKRWKKYGV HRLSFLERHC PPVYEKNECL
IPPPDGYKPP IRWPKSREQC WYRNVPYDWI NKQKSNQHWL KKEGDKFHFP GGGTMFPRGV
SHYVDLMQDL IPEMKDGTVR TAIDTGCGVA SWGGDLLDRG ILSLSLAPRD NHEAQVQFAL
ERGIPAILGI ISTQRLPFPS NAFDMAHCSR CLIPWTEFGG IYLLEIHRIV RPGGFWVLSG
PPVNYNRRWR GWNTTMEDQK SDYNKLQSLL TSMCFKKYAQ KDDIAVWQKL SDKSCYDKIA
KNMEAYPPKC DDSIEPDSAW YTPLRPCVVA PTPKVKKSGL GSIPKWPERL HVAPERIGDV
HGGSANSLKH DDGKWKNRVK HYKKVLPALG TDKIRNVMDM NTVYGGFSAA LIEDPIWVMN
VVSSYSANSL PVVFDRGLIG TYHDWCEAFS TYPRTYDLLH LDSLFTLESH RCEMKYILLE
MDRILRPSGY VIIRESSYFM DAITTLAKGI RWSCRREETE YAVKSEKILV CQKKLWFSSN
QTS
