PMTP_ARATH
ID PMTP_ARATH Reviewed; 770 AA.
AC Q0WT31; O80779;
DT 23-MAR-2010, integrated into UniProtKB/Swiss-Prot.
DT 23-MAR-2010, sequence version 2.
DT 03-AUG-2022, entry version 86.
DE RecName: Full=Probable methyltransferase PMT25;
DE EC=2.1.1.-;
GN OrderedLocusNames=At2g34300; ORFNames=F13P17.14;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia; TISSUE=Root;
RX PubMed=19423640; DOI=10.1093/dnares/dsp009;
RA Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M.,
RA Shinozaki K.;
RT "Analysis of multiple occurrences of alternative splicing events in
RT Arabidopsis thaliana using novel sequenced full-length cDNAs.";
RL DNA Res. 16:155-164(2009).
RN [5]
RP GENE FAMILY.
RX PubMed=17461780; DOI=10.1111/j.1365-313x.2007.03123.x;
RA Krupkova E., Immerzeel P., Pauly M., Schmulling T.;
RT "The TUMOROUS SHOOT DEVELOPMENT2 gene of Arabidopsis encoding a putative
RT methyltransferase is required for cell adhesion and co-ordinated plant
RT development.";
RL Plant J. 50:735-750(2007).
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000305}; Single-
CC pass type II membrane protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the methyltransferase superfamily.
CC {ECO:0000305}.
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DR EMBL; AC004481; AAC27406.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC08950.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC08951.1; -; Genomic_DNA.
DR EMBL; CP002685; ANM62567.1; -; Genomic_DNA.
DR EMBL; AK227732; BAE99717.1; -; mRNA.
DR EMBL; AK317495; BAH20160.1; -; mRNA.
DR PIR; T02318; T02318.
DR RefSeq; NP_001031477.1; NM_001036400.2.
DR RefSeq; NP_001324716.1; NM_001336501.1.
DR RefSeq; NP_180977.1; NM_128981.4.
DR AlphaFoldDB; Q0WT31; -.
DR STRING; 3702.AT2G34300.1; -.
DR PaxDb; Q0WT31; -.
DR PRIDE; Q0WT31; -.
DR ProteomicsDB; 234887; -.
DR EnsemblPlants; AT2G34300.1; AT2G34300.1; AT2G34300.
DR EnsemblPlants; AT2G34300.2; AT2G34300.2; AT2G34300.
DR EnsemblPlants; AT2G34300.3; AT2G34300.3; AT2G34300.
DR GeneID; 817991; -.
DR Gramene; AT2G34300.1; AT2G34300.1; AT2G34300.
DR Gramene; AT2G34300.2; AT2G34300.2; AT2G34300.
DR Gramene; AT2G34300.3; AT2G34300.3; AT2G34300.
DR KEGG; ath:AT2G34300; -.
DR Araport; AT2G34300; -.
DR TAIR; locus:2040864; AT2G34300.
DR eggNOG; ENOG502QTUG; Eukaryota.
DR HOGENOM; CLU_010485_1_2_1; -.
DR InParanoid; Q0WT31; -.
DR OMA; PPYWINS; -.
DR OrthoDB; 325808at2759; -.
DR PhylomeDB; Q0WT31; -.
DR PRO; PR:Q0WT31; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q0WT31; baseline and differential.
DR Genevisible; Q0WT31; AT.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005783; C:endoplasmic reticulum; HDA:TAIR.
DR GO; GO:0005768; C:endosome; HDA:TAIR.
DR GO; GO:0005794; C:Golgi apparatus; HDA:TAIR.
DR GO; GO:0005797; C:Golgi medial cisterna; HDA:TAIR.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009506; C:plasmodesma; HDA:TAIR.
DR GO; GO:0005802; C:trans-Golgi network; HDA:TAIR.
DR GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.150; -; 1.
DR InterPro; IPR004159; Put_SAM_MeTrfase.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR10108; PTHR10108; 1.
DR Pfam; PF03141; Methyltransf_29; 1.
DR SUPFAM; SSF53335; SSF53335; 2.
PE 2: Evidence at transcript level;
KW Glycoprotein; Golgi apparatus; Membrane; Methyltransferase;
KW Reference proteome; Signal-anchor; Transferase; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..770
FT /note="Probable methyltransferase PMT25"
FT /id="PRO_0000393265"
FT TOPO_DOM 1..17
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 18..38
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 39..770
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT REGION 44..238
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 58..89
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 90..111
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 112..191
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 201..238
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 75
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 107
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 163
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 178
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 244
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 363
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 328
FT /note="S -> G (in Ref. 3; BAE99717 and 4; BAH20160)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 770 AA; 86881 MW; B3571A6A7299F4E8 CRC64;
MAMGKYSRVD GKKSSSYGLT ITIVLLLSLC LVGTWMFMSS WSAPADSAGY SSTDTAKDVS
KNDLRKEEGD RDPKNFSDEK NEENEAATEN NQVKTDSENS AEGNQVNESS GEKTEAGEER
KESDDNNGDG DGEKEKNVKE VGSESDETTQ KEKTQLEEST EENKSEDGNG NEEKAEENAS
ETEESTEKSS KEVFPAGDQA EITKESSTGD GAWSTQLVES QNEKKAQQSS ISKDQSSYGW
KTCNVTAGPD YIPCLDNWQA IKKLHTTMHY EHRERHCPEE SPHCLVSLPD GYKRSIKWPK
SREKIWYNNV PHTKLAEIKG HQNWVKMSGE HLTFPGGGTQ FKNGALHYID FIQQSHPAIA
WGNRTRVILD VGCGVASFGG YLFERDVLAL SFAPKDEHEA QVQFALERGI PAMLNVMGTK
RLPFPGSVFD LIHCARCRVP WHIEGGKLLL ELNRALRPGG FFVWSATPVY RKNEEDSGIW
KAMSELTKAM CWKLVTIKKD KLNEVGAAIY QKPTSNKCYN KRPQNEPPLC KDSDDQNAAW
NVPLEACMHK VTEDSSKRGA VWPNMWPERV ETAPEWLDSQ EGVYGKPAPE DFTADQEKWK
TIVSKAYLND MGIDWSNVRN VMDMRAVYGG FAAALKDLKL WVMNVVPVDA PDTLPIIYER
GLFGIYHDWC ESFNTYPRTY DLLHADHLFS TLRKRCNLVS VMAEIDRILR PQGTFIIRDD
METLGEVEKM VKSMKWKVKM TQSKDNEGLL SIEKSWWRPE ETETIKSAIA
