ID   PN3B_PAMNI              Reviewed;          35 AA.
AC   P0DM13;
DT   23-MAY-2018, integrated into UniProtKB/Swiss-Prot.
DT   23-MAY-2018, sequence version 1.
DT   25-MAY-2022, entry version 9.
DE   RecName: Full=Mu-theraphotoxin-Pn3b {ECO:0000303|PubMed:28106092};
DE            Short=Mu-TRTX-Pn3b {ECO:0000303|PubMed:28106092};
OS   Pamphobeteus nigricolor (Giant blue bloom tarantula).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Mygalomorphae; Theraphosidae; Pamphobeteus.
OX   NCBI_TaxID=2083160;
RN   [1]
RP   PROTEIN SEQUENCE, MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RC   TISSUE=Venom;
RX   PubMed=28106092; DOI=10.1038/srep40883;
RA   Deuis J.R., Dekan Z., Wingerd J.S., Smith J.J., Munasinghe N.R.,
RA   Bhola R.F., Imlach W.L., Herzig V., Armstrong D.A., Rosengren K.J.,
RA   Bosmans F., Waxman S.G., Dib-Hajj S.D., Escoubas P., Minett M.S.,
RA   Christie M.J., King G.F., Alewood P.F., Lewis R.J., Wood J.N., Vetter I.;
RT   "Pharmacological characterisation of the highly NaV1.7 selective spider
RT   venom peptide Pn3a.";
RL   Sci. Rep. 7:40883-40883(2017).
RN   [2]
RP   ERRATUM OF PUBMED:28106092.
RX   PubMed=28548111; DOI=10.1038/srep46816;
RA   Deuis J.R., Dekan Z., Wingerd J.S., Smith J.J., Munasinghe N.R.,
RA   Bhola R.F., Imlach W.L., Herzig V., Armstrong D.A., Rosengren K.J.,
RA   Bosmans F., Waxman S.G., Dib-Hajj S.D., Escoubas P., Minett M.S.,
RA   Christie M.J., King G.F., Alewood P.F., Lewis R.J., Wood J.N., Vetter I.;
RT   "Corrigendum: Pharmacological characterisation of the highly NaV1.7
RT   selective spider venom peptide Pn3a.";
RL   Sci. Rep. 7:46816-46816(2017).
CC   -!- FUNCTION: Gating-modifier toxin that targets voltage-gated sodium
CC       channels with a preferential activity on Nav1.7/SCN9A. On Nav1.7/SCN9A,
CC       the toxin acts by shifting the voltage-dependence of activation to more
CC       depolarized potentials, whereas it does not cause significant effect on
CC       the voltage-dependence of activation on other sodium channels. Minor
CC       effects are observed on the voltage-dependence of steady-state fast
CC       inactivation for all sodium channels tested (Nav1.1/SCN1A-
CC       Nav1.8/SCN10A). By testing the toxin on channel chimera, it has been
CC       shown to interact with the S3-S4 linkers in DII and DIV domains of
CC       Nav1.7/SCN9A. In vivo, the toxin dose-dependently reduces OD1-induced
CC       spontaneous pain behaviors. {ECO:0000250|UniProtKB:P0DM12}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:28106092}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000305|PubMed:28106092}.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin.
CC       {ECO:0000250|UniProtKB:P0DM12}.
CC   -!- MASS SPECTROMETRY: Mass=4210.5; Method=MALDI; Note=Monoisotopic mass.;
CC       Evidence={ECO:0000269|PubMed:28106092};
CC   -!- MISCELLANEOUS: Shows no or weak effect on hNav1.8/SCN10A, rKv2.1/KCNB1
CC       and hCav1.2/CACNA1C, hCav2.2/CACNA1B, alpha-7/SCN9A nAChR, alpha-
CC       3/SCN3A nAChR. {ECO:0000250|UniProtKB:P0DM12}.
CC   -!- SIMILARITY: Belongs to the neurotoxin 10 (Hwtx-1) family. 28 (Jztx-11)
CC       subfamily. {ECO:0000305}.
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DR   AlphaFoldDB; P0DM13; -.
DR   SMR; P0DM13; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0008200; F:ion channel inhibitor activity; IEA:InterPro.
DR   GO; GO:0017080; F:sodium channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR011696; Huwentoxin-1.
DR   Pfam; PF07740; Toxin_12; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Ion channel impairing toxin;
KW   Knottin; Secreted; Toxin; Voltage-gated sodium channel impairing toxin.
FT   CHAIN           1..35
FT                   /note="Mu-theraphotoxin-Pn3b"
FT                   /evidence="ECO:0000269|PubMed:28106092"
FT                   /id="PRO_0000444152"
FT   DISULFID        2..16
FT                   /evidence="ECO:0000250|UniProtKB:P0DM12"
FT   DISULFID        9..21
FT                   /evidence="ECO:0000250|UniProtKB:P0DM12"
FT   DISULFID        15..28
FT                   /evidence="ECO:0000250|UniProtKB:P0DM12"
SQ   SEQUENCE   35 AA;  4220 MW;  134EAB43B203C373 CRC64;
     GCRYMFGDCE KDEDCCKHLG CKRKMKYCAW DFTFT