PNAA_PRUDU
ID PNAA_PRUDU Reviewed; 571 AA.
AC P81898; P81899;
DT 31-AUG-2004, integrated into UniProtKB/Swiss-Prot.
DT 07-DEC-2004, sequence version 2.
DT 25-MAY-2022, entry version 49.
DE RecName: Full=Peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase A;
DE Short=PNGase A;
DE EC=3.5.1.52;
DE AltName: Full=Glycopeptide N-glycosidase;
DE AltName: Full=N-glycanase;
DE Contains:
DE RecName: Full=Peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase A light chain;
DE AltName: Full=PNGase A small chain;
DE AltName: Full=PNGase A subunit B;
DE Contains:
DE RecName: Full=Peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase A heavy chain;
DE AltName: Full=PNGase A large chain;
DE Short=PNGase A subunit A;
OS Prunus dulcis (Almond) (Amygdalus dulcis).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Rosales; Rosaceae; Amygdaloideae; Amygdaleae; Prunus.
OX NCBI_TaxID=3755;
RN [1]
RP NUCLEOTIDE SEQUENCE, AND PROTEIN SEQUENCE OF 1-20; 165-184; 315-338;
RP 365-384; 386-409; 420-441; 443-463; 475-496 AND 498-536.
RC TISSUE=Fruit;
RA Hiroyuki I., Masanori M., Ikunoshin K.;
RT "Almond N-glycosidase gene.";
RL Patent number US5710016, 20-JAN-1998.
RN [2]
RP PROTEIN SEQUENCE OF 1-10 AND 165-175, SUBUNIT, AND MASS SPECTROMETRY.
RX PubMed=9523720; DOI=10.1046/j.1432-1327.1998.2520118.x;
RA Altmann F., Paschinger K., Dalik T., Vorauer K.;
RT "Characterisation of peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine
RT amidase A and its N-glycans.";
RL Eur. J. Biochem. 252:118-123(1998).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of an N(4)-(acetyl-beta-D-glucosaminyl)asparagine
CC residue in which the glucosamine residue may be further glycosylated,
CC to yield a (substituted) N-acetyl-beta-D-glucosaminylamine and a
CC peptide containing an aspartate residue.; EC=3.5.1.52;
CC -!- SUBUNIT: Heterodimer of a large and a small chain.
CC {ECO:0000269|PubMed:9523720}.
CC -!- PTM: Is highly glycosylated and is largly resistant against self-
CC deglycosylation.
CC -!- MASS SPECTROMETRY: [Peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine
CC amidase A light chain]: Mass=21247; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:9523720};
CC -!- MASS SPECTROMETRY: Mass=54182; Method=MALDI; Note=The measured range is
CC 165-?.; Evidence={ECO:0000269|PubMed:9523720};
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DR PIR; A59272; A59272.
DR PIR; B59272; B59272.
DR AlphaFoldDB; P81898; -.
DR BRENDA; 3.5.1.52; 5059.
DR GO; GO:0000224; F:peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase activity; IEA:UniProtKB-EC.
DR InterPro; IPR021102; PNGase_A.
DR PANTHER; PTHR31104; PTHR31104; 1.
DR Pfam; PF12222; PNGaseA; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Glycoprotein; Hydrolase.
FT CHAIN 1..?
FT /note="Peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine
FT amidase A light chain"
FT /id="PRO_0000022075"
FT CHAIN 165..571
FT /note="Peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine
FT amidase A heavy chain"
FT /id="PRO_0000022076"
FT CARBOHYD 121
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 143
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 197
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 241
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 318
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 367
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 390
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 423
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 457
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 481
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 524
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 529
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT UNSURE 27
FT /note="P or S"
FT UNSURE 74
FT /note="R or G"
FT UNSURE 152
FT /note="K or E"
FT UNSURE 179
FT /note="I or V"
FT UNSURE 488
FT /note="R or Q"
FT UNSURE 506
FT /note="E or G"
FT UNSURE 511
FT /note="I or V"
SQ SEQUENCE 571 AA; 64480 MW; 77E3F846C9E6CFC9 CRC64;
EPTPLHDTPP TVFFEVTKPI EVPKTKPCSQ LILQHDFAYT YGQAPVFANY TPPSDCPSQT
FSTIVLEWKA TCRRRQFDRI FGVWLGGVEI LRSCTAEPRP NGIVWTVEKD ITRYYSLLKS
NQTLAVYLGN LIDKTYTGIY HVNISLHFYP AKEKLNSFQQ KLDNLASGYH SWADLILPIS
RNLPLNDGLW FEVQNSNDTE LKEFKIPQNA YRAVLEVYVS FHENDEFWYS NLPNEYIAAN
NLSGTPGNGP FREVVVSLDG EVVGAVWPFT VIFTGGINPL LWRPITAIGS FDLPTYDIEI
TPFLGKILDG KSHKFGFNVT NALNVWYVDA NLHLWLDKQS TKTEGKLSKH SSLPLVVSLV
SDFKGLNGTF LTRTSRSVSS TGWVKSSYGN ITTRSIQDFY YSNSMVLGKD GNMQIVNQKI
IFNDSVYINL PSSYVHSLTS HKTFPLYLYT DFLGQGNGTY LLITNVDLGF IEKKSGLGFS
NSSLRNLRSA EGNMVVKNNL VVSGLESTQQ IYRYDGGKFC YFRNISSSNY TILYDKVGSK
CNKKSLSNLD FVLSRLWPFG ARMNFAGLRF T
