PNC1_ARATH
ID PNC1_ARATH Reviewed; 322 AA.
AC Q9MA90; Q8L9D8;
DT 09-JAN-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Peroxisomal adenine nucleotide carrier 1;
DE Short=AtPNC1;
GN Name=PNC1; OrderedLocusNames=At3g05290; ORFNames=T12H1.26;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, AND DEVELOPMENTAL STAGE.
RX PubMed=19073762; DOI=10.1105/tpc.108.062877;
RA Arai Y., Hayashi M., Nishimura M.;
RT "Proteomic identification and characterization of a novel peroxisomal
RT adenine nucleotide transporter supplying ATP for fatty acid beta-oxidation
RT in soybean and Arabidopsis.";
RL Plant Cell 20:3227-3240(2008).
RN [6]
RP FUNCTION, SUBCELLULAR LOCATION, BIOPHYSICOCHEMICAL PROPERTIES, AND TISSUE
RP SPECIFICITY.
RX PubMed=19073763; DOI=10.1105/tpc.108.062042;
RA Linka N., Theodoulou F.L., Haslam R.P., Linka M., Napier J.A.,
RA Neuhaus H.E., Weber A.P.;
RT "Peroxisomal ATP import is essential for seedling development in
RT Arabidopsis thaliana.";
RL Plant Cell 20:3241-3257(2008).
CC -!- FUNCTION: Peroxisomal adenine nucleotide transporter catalyzing the
CC counterexchange of ATP with AMP. ATP is needed by reactions that
CC generate acyl-CoA for peroxisomal fatty acid beta-oxidation during
CC postgerminative growth. Required for the beta-oxidation reactions
CC involved in auxin biosynthesis and for the conversion of seed-reserved
CC triacylglycerols into sucrose that is necessary for growth before the
CC onset of photosynthesis. {ECO:0000269|PubMed:19073762,
CC ECO:0000269|PubMed:19073763}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=83 uM for ATP {ECO:0000269|PubMed:19073763};
CC -!- SUBCELLULAR LOCATION: Peroxisome membrane {ECO:0000269|PubMed:19073762,
CC ECO:0000269|PubMed:19073763}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:19073762, ECO:0000269|PubMed:19073763}.
CC -!- TISSUE SPECIFICITY: Expressed in stamens, pollen grains, seeds, leaves,
CC cotyledons, roots, stems, flowers, hypocotyls and siliques.
CC {ECO:0000269|PubMed:19073763}.
CC -!- DEVELOPMENTAL STAGE: Expressed in the early seedling stage of post-
CC germinative growth. {ECO:0000269|PubMed:19073762}.
CC -!- SIMILARITY: Belongs to the mitochondrial carrier (TC 2.A.29) family.
CC {ECO:0000305}.
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DR EMBL; AC009177; AAF27035.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE74216.1; -; Genomic_DNA.
DR EMBL; AY048291; AAK82553.1; -; mRNA.
DR EMBL; BT000838; AAN38675.1; -; mRNA.
DR EMBL; AY088489; AAM66025.1; -; mRNA.
DR RefSeq; NP_566251.1; NM_111402.5.
DR AlphaFoldDB; Q9MA90; -.
DR SMR; Q9MA90; -.
DR STRING; 3702.AT3G05290.1; -.
DR TCDB; 2.A.29.20.3; the mitochondrial carrier (mc) family.
DR PaxDb; Q9MA90; -.
DR PRIDE; Q9MA90; -.
DR ProteomicsDB; 234879; -.
DR EnsemblPlants; AT3G05290.1; AT3G05290.1; AT3G05290.
DR GeneID; 819693; -.
DR Gramene; AT3G05290.1; AT3G05290.1; AT3G05290.
DR KEGG; ath:AT3G05290; -.
DR Araport; AT3G05290; -.
DR TAIR; locus:2096344; AT3G05290.
DR eggNOG; KOG0769; Eukaryota.
DR HOGENOM; CLU_015166_6_3_1; -.
DR InParanoid; Q9MA90; -.
DR OMA; PLEMINT; -.
DR OrthoDB; 1126848at2759; -.
DR PhylomeDB; Q9MA90; -.
DR PRO; PR:Q9MA90; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9MA90; baseline and differential.
DR Genevisible; Q9MA90; AT.
DR GO; GO:0005779; C:integral component of peroxisomal membrane; IBA:GO_Central.
DR GO; GO:0005777; C:peroxisome; IDA:TAIR.
DR GO; GO:0015217; F:ADP transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0015297; F:antiporter activity; IEA:UniProtKB-KW.
DR GO; GO:0005347; F:ATP transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0015866; P:ADP transport; IDA:TAIR.
DR GO; GO:0015867; P:ATP transport; IDA:TAIR.
DR GO; GO:0006635; P:fatty acid beta-oxidation; IMP:TAIR.
DR GO; GO:0080024; P:indolebutyric acid metabolic process; IMP:TAIR.
DR GO; GO:0007031; P:peroxisome organization; IBA:GO_Central.
DR GO; GO:0090351; P:seedling development; IMP:UniProtKB.
DR Gene3D; 1.50.40.10; -; 1.
DR InterPro; IPR018108; Mitochondrial_sb/sol_carrier.
DR InterPro; IPR023395; Mt_carrier_dom_sf.
DR InterPro; IPR045900; Peroxisomal_Ade_carrier.
DR PANTHER; PTHR46650; PTHR46650; 1.
DR Pfam; PF00153; Mito_carr; 3.
DR SUPFAM; SSF103506; SSF103506; 1.
DR PROSITE; PS50920; SOLCAR; 3.
PE 1: Evidence at protein level;
KW Antiport; Membrane; Peroxisome; Reference proteome; Repeat; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..322
FT /note="Peroxisomal adenine nucleotide carrier 1"
FT /id="PRO_0000420691"
FT TRANSMEM 8..28
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 104..124
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TRANSMEM 158..178
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TRANSMEM 201..221
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TRANSMEM 254..274
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TRANSMEM 286..306
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT REPEAT 5..94
FT /note="Solcar 1"
FT REPEAT 104..184
FT /note="Solcar 2"
FT REPEAT 202..298
FT /note="Solcar 3"
FT CONFLICT 62
FT /note="Q -> H (in Ref. 4; AAM66025)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 322 AA; 35551 MW; 42514037F5E520A0 CRC64;
MGVDLESVSE ATSGAIGSLL STTILYPLDT CKSKFQAEVR ARGQQKYRYL SDVMWEAISK
GQVFSLYQGL GTKNFQSFIS QFIYFYSYSY FKRVHSERTG SKSIGTKANL LIAAAAGACT
SVLIQPLDTA SSRMQTSEFG ESKGLWKTLT EGSWADAFDG LGISLLLTSN PAIQYTVFDQ
LKQHLLKQKN AKAENGSSPV VLSAFMAFVL GAVSKSVATV LTYPAIRCKV MIQAADESKE
NETKKPRRRT RKTIPGVVYA IWRKEGMLGF FKGLQAQILK TVLSSALLLM IKEKITATTW
ILILAIRRTL FLTNTKGKLR SP
