PNC2_ARATH
ID PNC2_ARATH Reviewed; 321 AA.
AC Q8VZS0;
DT 09-JAN-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=Peroxisomal adenine nucleotide carrier 2;
GN Name=PNC2; OrderedLocusNames=At5g27520; ORFNames=F15A18, F21A20.230;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, AND DEVELOPMENTAL STAGE.
RX PubMed=19073762; DOI=10.1105/tpc.108.062877;
RA Arai Y., Hayashi M., Nishimura M.;
RT "Proteomic identification and characterization of a novel peroxisomal
RT adenine nucleotide transporter supplying ATP for fatty acid beta-oxidation
RT in soybean and Arabidopsis.";
RL Plant Cell 20:3227-3240(2008).
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, BIOPHYSICOCHEMICAL PROPERTIES, AND TISSUE
RP SPECIFICITY.
RX PubMed=19073763; DOI=10.1105/tpc.108.062042;
RA Linka N., Theodoulou F.L., Haslam R.P., Linka M., Napier J.A.,
RA Neuhaus H.E., Weber A.P.;
RT "Peroxisomal ATP import is essential for seedling development in
RT Arabidopsis thaliana.";
RL Plant Cell 20:3241-3257(2008).
CC -!- FUNCTION: Peroxisomal adenine nucleotide transporter catalyzing the
CC counterexchange of ATP with AMP. ATP is needed by reactions that
CC generate acyl-CoA for peroxisomal fatty acid beta-oxidation during
CC postgerminative growth. Required for the beta-oxidation reactions
CC involved in auxin biosynthesis and for the conversion of seed-reserved
CC triacylglycerols into sucrose that is necessary for growth before the
CC onset of photosynthesis. {ECO:0000269|PubMed:19073762,
CC ECO:0000269|PubMed:19073763}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=150 uM for ATP {ECO:0000269|PubMed:19073763};
CC -!- SUBCELLULAR LOCATION: Peroxisome membrane {ECO:0000269|PubMed:19073762,
CC ECO:0000269|PubMed:19073763}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:19073762, ECO:0000269|PubMed:19073763}.
CC -!- TISSUE SPECIFICITY: Expressed in stamens, pollen grains, seeds, leaves,
CC cotyledons, roots, stems, flowers, hypocotyls and siliques.
CC {ECO:0000269|PubMed:19073763}.
CC -!- DEVELOPMENTAL STAGE: Expressed in the early seedling stage of post-
CC germinative growth. {ECO:0000269|PubMed:19073762}.
CC -!- SIMILARITY: Belongs to the mitochondrial carrier (TC 2.A.29) family.
CC {ECO:0000305}.
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DR EMBL; AC007123; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC007478; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002688; AED93696.1; -; Genomic_DNA.
DR EMBL; AY063892; AAL36248.1; -; mRNA.
DR EMBL; AY122919; AAM67452.1; -; mRNA.
DR RefSeq; NP_198104.1; NM_122634.4.
DR AlphaFoldDB; Q8VZS0; -.
DR SMR; Q8VZS0; -.
DR STRING; 3702.AT5G27520.1; -.
DR TCDB; 2.A.29.20.2; the mitochondrial carrier (mc) family.
DR PaxDb; Q8VZS0; -.
DR PRIDE; Q8VZS0; -.
DR ProteomicsDB; 234986; -.
DR EnsemblPlants; AT5G27520.1; AT5G27520.1; AT5G27520.
DR GeneID; 832812; -.
DR Gramene; AT5G27520.1; AT5G27520.1; AT5G27520.
DR KEGG; ath:AT5G27520; -.
DR Araport; AT5G27520; -.
DR TAIR; locus:2146355; AT5G27520.
DR eggNOG; KOG0769; Eukaryota.
DR HOGENOM; CLU_015166_6_3_1; -.
DR InParanoid; Q8VZS0; -.
DR OMA; CNIWKKE; -.
DR OrthoDB; 1126848at2759; -.
DR PhylomeDB; Q8VZS0; -.
DR PRO; PR:Q8VZS0; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q8VZS0; baseline and differential.
DR Genevisible; Q8VZS0; AT.
DR GO; GO:0005779; C:integral component of peroxisomal membrane; IBA:GO_Central.
DR GO; GO:0005777; C:peroxisome; IDA:TAIR.
DR GO; GO:0015217; F:ADP transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0015297; F:antiporter activity; IEA:UniProtKB-KW.
DR GO; GO:0005347; F:ATP transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0015866; P:ADP transport; IDA:TAIR.
DR GO; GO:0015867; P:ATP transport; IDA:TAIR.
DR GO; GO:0006635; P:fatty acid beta-oxidation; IMP:TAIR.
DR GO; GO:0080024; P:indolebutyric acid metabolic process; IMP:TAIR.
DR GO; GO:0007031; P:peroxisome organization; IBA:GO_Central.
DR Gene3D; 1.50.40.10; -; 1.
DR InterPro; IPR018108; Mitochondrial_sb/sol_carrier.
DR InterPro; IPR023395; Mt_carrier_dom_sf.
DR InterPro; IPR045900; Peroxisomal_Ade_carrier.
DR PANTHER; PTHR46650; PTHR46650; 1.
DR Pfam; PF00153; Mito_carr; 3.
DR SUPFAM; SSF103506; SSF103506; 1.
DR PROSITE; PS50920; SOLCAR; 3.
PE 1: Evidence at protein level;
KW Antiport; Membrane; Peroxisome; Reference proteome; Repeat; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..321
FT /note="Peroxisomal adenine nucleotide carrier 2"
FT /id="PRO_0000420693"
FT TRANSMEM 10..30
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 106..126
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TRANSMEM 160..180
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TRANSMEM 203..223
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TRANSMEM 256..276
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TRANSMEM 288..308
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT REPEAT 7..96
FT /note="Solcar 1"
FT REPEAT 106..186
FT /note="Solcar 2"
FT REPEAT 204..300
FT /note="Solcar 3"
SQ SEQUENCE 321 AA; 35159 MW; D8E8E94745E49079 CRC64;
MGVDLDLESI SEATSGAIGS LLSTTILYPL DTCKSKFQAE IRVRGQQKYR YLSDVFWEAI
SSGNVLSLYQ GLGTKNLQSF ISSFIYFYSY SYFKRLHSQR IGSKSIGTKA NLLIAAAAGA
CTSVLTQPLD TASSRMQTSE FGKSKGLWKT LTDGSWGNAF DGLGISLLLT SNPAIQYTVF
DQLKQNLLEK GKAKSNKDSS PVVLSAFMAF VLGAVSKSAA TVITYPAIRC KVMIQAADDS
KENEAKKPRK RIRKTIPGVV YAIWKKEGIL GFFKGLQAQI LKTVLSSALL LMIKEKITAT
TWILILAIRT LFVTKARLKS P
