ID   PNCC_ECOL6              Reviewed;         165 AA.
AC   P0A6G4; P41053;
DT   29-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   29-MAR-2005, sequence version 1.
DT   25-MAY-2022, entry version 87.
DE   RecName: Full=Nicotinamide-nucleotide amidohydrolase PncC;
DE            Short=NMN amidohydrolase PncC;
DE            EC=3.5.1.42;
DE   AltName: Full=NMN deamidase;
DE   AltName: Full=Nicotinamide-nucleotide amidase;
GN   Name=pncC; OrderedLocusNames=c3254;
OS   Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=199310;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CFT073 / ATCC 700928 / UPEC;
RX   PubMed=12471157; DOI=10.1073/pnas.252529799;
RA   Welch R.A., Burland V., Plunkett G. III, Redford P., Roesch P., Rasko D.,
RA   Buckles E.L., Liou S.-R., Boutin A., Hackett J., Stroud D., Mayhew G.F.,
RA   Rose D.J., Zhou S., Schwartz D.C., Perna N.T., Mobley H.L.T.,
RA   Donnenberg M.S., Blattner F.R.;
RT   "Extensive mosaic structure revealed by the complete genome sequence of
RT   uropathogenic Escherichia coli.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:17020-17024(2002).
CC   -!- FUNCTION: Has NMN aminohydrolase activity, not active on other
CC       substrates. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=beta-nicotinamide D-ribonucleotide + H2O = NH4(+) + nicotinate
CC         beta-D-ribonucleotide; Xref=Rhea:RHEA:12400, ChEBI:CHEBI:14649,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:28938, ChEBI:CHEBI:57502; EC=3.5.1.42;
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the CinA family. PncC subfamily. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAN81705.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AE014075; AAN81705.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_000132231.1; NC_004431.1.
DR   AlphaFoldDB; P0A6G4; -.
DR   SMR; P0A6G4; -.
DR   STRING; 199310.c3254; -.
DR   EnsemblBacteria; AAN81705; AAN81705; c3254.
DR   GeneID; 66673431; -.
DR   KEGG; ecc:c3254; -.
DR   eggNOG; COG1546; Bacteria.
DR   HOGENOM; CLU_030805_1_1_6; -.
DR   OMA; VEHALNM; -.
DR   Proteomes; UP000001410; Chromosome.
DR   GO; GO:0019159; F:nicotinamide-nucleotide amidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0019363; P:pyridine nucleotide biosynthetic process; IEA:UniProtKB-KW.
DR   Gene3D; 3.90.950.20; -; 1.
DR   InterPro; IPR036653; CinA-like_C.
DR   InterPro; IPR008136; CinA_C.
DR   Pfam; PF02464; CinA; 1.
DR   SUPFAM; SSF142433; SSF142433; 1.
DR   TIGRFAMs; TIGR00199; PncC_domain; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Pyridine nucleotide biosynthesis.
FT   CHAIN           1..165
FT                   /note="Nicotinamide-nucleotide amidohydrolase PncC"
FT                   /id="PRO_0000156791"
SQ   SEQUENCE   165 AA;  17582 MW;  9B6D41D25D5DE212 CRC64;
     MTDSELMQLS EQVGQALKAR GATVTTAESC TGGWVAKVIT DIAGSSAWFE RGFVTYSNEA
     KAQMIGVREE TLAQHGAVSE PVVVEMAIGA LKAARADYAV SISGIAGPDG GSEEKPVGTV
     WFAFATARGE GITRRECFSG DRDAVRRQAT AYALQTLWQQ FLQNT