PNCC_ECOLI
ID PNCC_ECOLI Reviewed; 165 AA.
AC P0A6G3; P41053;
DT 29-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 29-MAR-2005, sequence version 1.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=Nicotinamide-nucleotide amidohydrolase PncC;
DE Short=NMN amidohydrolase PncC;
DE EC=3.5.1.42;
DE AltName: Full=NMN deamidase;
DE AltName: Full=Nicotinamide-nucleotide amidase;
GN Name=pncC; Synonyms=ygaD; OrderedLocusNames=b2700, JW2670;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=7476170; DOI=10.1111/j.1365-2958.1995.tb02437.x;
RA Ehlert K., Hoeltje J.-V., Templin M.F.;
RT "Cloning and expression of a murein hydrolase lipoprotein from Escherichia
RT coli.";
RL Mol. Microbiol. 16:761-768(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9205837; DOI=10.1093/dnares/4.2.91;
RA Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T.,
RA Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K.,
RA Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T., Oyama S.,
RA Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H.,
RA Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.;
RT "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12
RT genome corresponding to 50.0-68.8 min on the linkage map and analysis of
RT its sequence features.";
RL DNA Res. 4:91-113(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP PRELIMINARY NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 114-165.
RX PubMed=6930655; DOI=10.1073/pnas.77.5.2611;
RA Sancar A., Stachelek C., Konigsberg W., Rupp W.D.;
RT "Sequences of the recA gene and protein.";
RL Proc. Natl. Acad. Sci. U.S.A. 77:2611-2615(1980).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY.
RC STRAIN=B / BL21;
RX PubMed=10493123;
RX DOI=10.1002/(sici)1522-2683(19990801)20:11<2181::aid-elps2181>3.0.co;2-q;
RA Fountoulakis M., Takacs M.-F., Berndt P., Langen H., Takacs B.;
RT "Enrichment of low abundance proteins of Escherichia coli by hydroxyapatite
RT chromatography.";
RL Electrophoresis 20:2181-2195(1999).
RN [7]
RP FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, AND SUBUNIT.
RC STRAIN=K12;
RX PubMed=21953451; DOI=10.1074/jbc.m111.275818;
RA Galeazzi L., Bocci P., Amici A., Brunetti L., Ruggieri S., Romine M.,
RA Reed S., Osterman A.L., Rodionov D.A., Sorci L., Raffaelli N.;
RT "Identification of nicotinamide mononucleotide deamidase of the bacterial
RT pyridine nucleotide cycle reveals a novel broadly conserved amidohydrolase
RT family.";
RL J. Biol. Chem. 286:40365-40375(2011).
CC -!- FUNCTION: Has nicotinamidemononucleotide (NMN) aminohydrolase activity,
CC not active on other substrates. {ECO:0000269|PubMed:21953451}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=beta-nicotinamide D-ribonucleotide + H2O = NH4(+) + nicotinate
CC beta-D-ribonucleotide; Xref=Rhea:RHEA:12400, ChEBI:CHEBI:14649,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:28938, ChEBI:CHEBI:57502; EC=3.5.1.42;
CC Evidence={ECO:0000269|PubMed:21953451};
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=6 uM for NMN {ECO:0000269|PubMed:21953451};
CC Note=kcat is 3.3 sec(-1).;
CC pH dependence:
CC Optimum pH active over a broad range of pH values.
CC {ECO:0000269|PubMed:21953451};
CC -!- SUBUNIT: Homodimer. {ECO:0000305|PubMed:21953451}.
CC -!- SIMILARITY: Belongs to the CinA family. PncC subfamily. {ECO:0000305}.
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DR EMBL; U18785; AAB60061.1; -; Genomic_DNA.
DR EMBL; U00096; AAC75742.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA16562.1; -; Genomic_DNA.
DR EMBL; V00328; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR PIR; H65049; H65049.
DR RefSeq; NP_417180.1; NC_000913.3.
DR RefSeq; WP_000132231.1; NZ_STEB01000027.1.
DR AlphaFoldDB; P0A6G3; -.
DR SMR; P0A6G3; -.
DR BioGRID; 4262070; 139.
DR DIP; DIP-48189N; -.
DR IntAct; P0A6G3; 9.
DR STRING; 511145.b2700; -.
DR jPOST; P0A6G3; -.
DR PaxDb; P0A6G3; -.
DR PRIDE; P0A6G3; -.
DR EnsemblBacteria; AAC75742; AAC75742; b2700.
DR EnsemblBacteria; BAA16562; BAA16562; BAA16562.
DR GeneID; 66673431; -.
DR GeneID; 947169; -.
DR KEGG; ecj:JW2670; -.
DR KEGG; eco:b2700; -.
DR PATRIC; fig|1411691.4.peg.4042; -.
DR EchoBASE; EB2560; -.
DR eggNOG; COG1546; Bacteria.
DR HOGENOM; CLU_030805_1_1_6; -.
DR InParanoid; P0A6G3; -.
DR OMA; VEHALNM; -.
DR PhylomeDB; P0A6G3; -.
DR BioCyc; EcoCyc:G7409-MON; -.
DR BioCyc; MetaCyc:G7409-MON; -.
DR PRO; PR:P0A6G3; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0019159; F:nicotinamide-nucleotide amidase activity; IDA:EcoCyc.
DR GO; GO:0019363; P:pyridine nucleotide biosynthetic process; IEA:UniProtKB-KW.
DR Gene3D; 3.90.950.20; -; 1.
DR InterPro; IPR036653; CinA-like_C.
DR InterPro; IPR008136; CinA_C.
DR Pfam; PF02464; CinA; 1.
DR SUPFAM; SSF142433; SSF142433; 1.
DR TIGRFAMs; TIGR00199; PncC_domain; 1.
PE 1: Evidence at protein level;
KW Hydrolase; Pyridine nucleotide biosynthesis; Reference proteome.
FT CHAIN 1..165
FT /note="Nicotinamide-nucleotide amidohydrolase PncC"
FT /id="PRO_0000156790"
SQ SEQUENCE 165 AA; 17582 MW; 9B6D41D25D5DE212 CRC64;
MTDSELMQLS EQVGQALKAR GATVTTAESC TGGWVAKVIT DIAGSSAWFE RGFVTYSNEA
KAQMIGVREE TLAQHGAVSE PVVVEMAIGA LKAARADYAV SISGIAGPDG GSEEKPVGTV
WFAFATARGE GITRRECFSG DRDAVRRQAT AYALQTLWQQ FLQNT
