ID   PNCC_PSEPU              Reviewed;         160 AA.
AC   P72227;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   25-MAY-2022, entry version 69.
DE   RecName: Full=Nicotinamide-nucleotide amidohydrolase PncC;
DE            Short=NMN amidohydrolase PncC;
DE            EC=3.5.1.42;
DE   AltName: Full=NMN deamidase;
DE   AltName: Full=Nicotinamide-nucleotide amidase;
GN   Name=pncC;
OS   Pseudomonas putida (Arthrobacter siderocapsulatus).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=303;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=PPS145;
RA   Yan M., McBeth D.L.;
RT   "Cloning, DNA sequencing, and characterization of the Pseudomonas putida
RT   PpS145 recA gene, recA-associated gene and recA region.";
RL   Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Has NMN aminohydrolase activity, not active on other
CC       substrates. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=beta-nicotinamide D-ribonucleotide + H2O = NH4(+) + nicotinate
CC         beta-D-ribonucleotide; Xref=Rhea:RHEA:12400, ChEBI:CHEBI:14649,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:28938, ChEBI:CHEBI:57502; EC=3.5.1.42;
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the CinA family. PncC subfamily. {ECO:0000305}.
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DR   EMBL; U70864; AAB16920.1; -; Genomic_DNA.
DR   PIR; T10481; T10481.
DR   AlphaFoldDB; P72227; -.
DR   SMR; P72227; -.
DR   STRING; 1240350.AMZE01000013_gene3500; -.
DR   PRIDE; P72227; -.
DR   eggNOG; COG1546; Bacteria.
DR   GO; GO:0019159; F:nicotinamide-nucleotide amidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0019363; P:pyridine nucleotide biosynthetic process; IEA:UniProtKB-KW.
DR   Gene3D; 3.90.950.20; -; 1.
DR   InterPro; IPR036653; CinA-like_C.
DR   InterPro; IPR008136; CinA_C.
DR   Pfam; PF02464; CinA; 1.
DR   SUPFAM; SSF142433; SSF142433; 1.
DR   TIGRFAMs; TIGR00199; PncC_domain; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Pyridine nucleotide biosynthesis.
FT   CHAIN           1..160
FT                   /note="Nicotinamide-nucleotide amidohydrolase PncC"
FT                   /id="PRO_0000156794"
SQ   SEQUENCE   160 AA;  16736 MW;  29A955AC576BB64F CRC64;
     MDPITTLATR LGEHLRRFNA QVTTAESCTG GGIAEAITRV PGSSAWFEAG YVTYSNAQKT
     RQLGVPEVLF GQVGAVSQEV VEAMVRGAQA ASGARFAVAV SGVAGPDGGS PAKPVGTVWL
     AWGDGSRVFS ERRQFDGDRE AVRRQTVIAA LDGLLQLGTE