ID   PNDA1_ECOLX             Reviewed;          50 AA.
AC   P11902;
DT   01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1989, sequence version 1.
DT   25-MAY-2022, entry version 88.
DE   RecName: Full=Protein PndA;
GN   Name=pndA; Synonyms=pnd;
OS   Escherichia coli.
OG   Plasmid R16.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND INDUCTION.
RX   PubMed=2465777; DOI=10.1016/0167-4781(89)90034-1;
RA   Sakikawa T., Akimoto S., Ohnishi Y.;
RT   "The pnd gene in E. coli plasmid R16: nucleotide sequence and gene
RT   expression leading to cell Mg2+ release and stable RNA degradation.";
RL   Biochim. Biophys. Acta 1007:158-166(1989).
CC   -!- FUNCTION: When overexpressed kill the cells from the inside by
CC       interfering with a vital function in the cell membrane.
CC   -!- FUNCTION: Toxic component of a type I toxin-antitoxin (TA) system (By
CC       similarity). Toxic component of a type I toxin-antitoxin (TA) system
CC       (By similarity). When expressed is involved in cellular Mg(2+) release
CC       and degradation of stable RNA (PubMed:2465777).
CC       {ECO:0000250|UniProtKB:P0ACG4, ECO:0000269|PubMed:2465777}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane
CC       {ECO:0000250|UniProtKB:P0ACG4}; Single-pass membrane protein
CC       {ECO:0000305}.
CC   -!- INDUCTION: In the presence of rifampicin at 42 degrees Celsius.
CC       {ECO:0000269|PubMed:2465777}.
CC   -!- SIMILARITY: Belongs to the Hok/Gef family. {ECO:0000305}.
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DR   EMBL; X12833; CAA31320.1; -; Genomic_DNA.
DR   PIR; S02763; S02763.
DR   RefSeq; WP_001302699.1; NZ_WWEL01000051.1.
DR   AlphaFoldDB; P11902; -.
DR   SMR; P11902; -.
DR   PATRIC; fig|562.11480.peg.1419; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR000021; Hok/gef_toxin.
DR   InterPro; IPR018084; Hok/gef_toxin_CS.
DR   Pfam; PF01848; HOK_GEF; 1.
DR   PRINTS; PR00281; HOKGEFTOXIC.
DR   PROSITE; PS00556; HOK_GEF; 1.
PE   2: Evidence at transcript level;
KW   Cell inner membrane; Cell membrane; Membrane; Plasmid;
KW   Toxin-antitoxin system; Transmembrane; Transmembrane helix.
FT   CHAIN           1..50
FT                   /note="Protein PndA"
FT                   /id="PRO_0000199043"
FT   TRANSMEM        5..25
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   50 AA;  5796 MW;  F4757270A3F21792 CRC64;
     MPQRTFLMML IVVCVTILCF VWMVRDSLCG FRIEQGNTVL VATLAYEVKR