ID   PNH1_ORYSJ              Reviewed;         979 AA.
AC   Q69VD5; Q0DB71; Q8LP00;
DT   07-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2004, sequence version 1.
DT   03-AUG-2022, entry version 80.
DE   RecName: Full=Protein argonaute PNH1;
DE   AltName: Full=Protein PINHEAD homolog 1;
DE            Short=OsPNH1;
GN   Name=PHN1; OrderedLocusNames=Os06g0597400, LOC_Os06g39640;
GN   ORFNames=P0417D05.31;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL
RP   STAGE, AND DISRUPTION PHENOTYPE.
RC   STRAIN=cv. Taichung 65; TISSUE=Embryo;
RX   PubMed=12000455; DOI=10.1046/j.1365-313x.2002.01279.x;
RA   Nishimura A., Ito M., Kamiya N., Sato Y., Matsuoka M.;
RT   "OsPNH1 regulates leaf development and maintenance of the shoot apical
RT   meristem in rice.";
RL   Plant J. 30:189-201(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [5]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=18826656; DOI=10.1186/1471-2164-9-451;
RA   Kapoor M., Arora R., Lama T., Nijhawan A., Khurana J.P., Tyagi A.K.,
RA   Kapoor S.;
RT   "Genome-wide identification, organization and phylogenetic analysis of
RT   dicer-like, argonaute and RNA-dependent RNA polymerase gene families and
RT   their expression analysis during reproductive development and stress in
RT   rice.";
RL   BMC Genomics 9:451-451(2008).
CC   -!- FUNCTION: Probably involved in the RNA silencing pathway. May bind to
CC       short RNAs such as microRNAs (miRNAs) or short interfering RNAs
CC       (siRNAs), and represses the translation of mRNAs which are
CC       complementary to them (By similarity). Plays a role in the maintenance
CC       of the indeterminate state of the stem cells in the shoot apical
CC       meristem (SAM). Regulates leaf formation through vascular development
CC       and may be involved in determining the central domain of the leaf
CC       founder region. {ECO:0000250, ECO:0000269|PubMed:12000455}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:12000455}.
CC   -!- DEVELOPMENTAL STAGE: Expressed in the center of the leaf primordia at
CC       plastochron 1 (P1) stage. In the P2 leaf primordia, expressed in the
CC       mid-vein and the future large veins. In P3 and P4, expressed in the
CC       vascular regions and the future bundle sheath extension cells.
CC       Expression in leaf primordia quickly disappears after P4 stage. In the
CC       inflorescence meristem, expressed at the predicted site of the next
CC       bract, and then in the vascular tissue of the developing rachis-branch.
CC       In developing floral organs, expressed in the adaxial side of the
CC       palea, lemma and stamen. {ECO:0000269|PubMed:12000455}.
CC   -!- DISRUPTION PHENOTYPE: Malformed leaves with an altered vascular
CC       arrangement and abnormal internal structure. Aberrant SAM with reduced
CC       OSH1 gene expression. {ECO:0000269|PubMed:12000455}.
CC   -!- SIMILARITY: Belongs to the argonaute family. Ago subfamily.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAF19902.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AB081950; BAB96813.1; -; mRNA.
DR   EMBL; AP004236; BAD33046.1; -; Genomic_DNA.
DR   EMBL; AP008212; BAF19902.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AP014962; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   RefSeq; XP_015643801.1; XM_015788315.1.
DR   RefSeq; XP_015643802.1; XM_015788316.1.
DR   AlphaFoldDB; Q69VD5; -.
DR   SMR; Q69VD5; -.
DR   STRING; 39947.Q69VD5; -.
DR   PaxDb; Q69VD5; -.
DR   PRIDE; Q69VD5; -.
DR   EnsemblPlants; Os06t0597400-01; Os06t0597400-01; Os06g0597400.
DR   GeneID; 9269490; -.
DR   Gramene; Os06t0597400-01; Os06t0597400-01; Os06g0597400.
DR   KEGG; osa:9269490; -.
DR   InParanoid; Q69VD5; -.
DR   OrthoDB; 159407at2759; -.
DR   PlantReactome; R-OSA-9627657; Regulation of leaf development.
DR   Proteomes; UP000000763; Chromosome 6.
DR   Proteomes; UP000059680; Chromosome 6.
DR   GO; GO:0005737; C:cytoplasm; IDA:Gramene.
DR   GO; GO:0004521; F:endoribonuclease activity; IBA:GO_Central.
DR   GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR   GO; GO:0048366; P:leaf development; IMP:Gramene.
DR   GO; GO:0035194; P:post-transcriptional gene silencing by RNA; IBA:GO_Central.
DR   GO; GO:0019827; P:stem cell population maintenance; IMP:Gramene.
DR   CDD; cd04657; Piwi_ago-like; 1.
DR   Gene3D; 3.30.420.10; -; 1.
DR   InterPro; IPR014811; ArgoL1.
DR   InterPro; IPR032472; ArgoL2.
DR   InterPro; IPR032473; Argonaute_Mid_dom.
DR   InterPro; IPR032474; Argonaute_N.
DR   InterPro; IPR003100; PAZ_dom.
DR   InterPro; IPR036085; PAZ_dom_sf.
DR   InterPro; IPR003165; Piwi.
DR   InterPro; IPR045246; Piwi_ago-like.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   InterPro; IPR036397; RNaseH_sf.
DR   Pfam; PF08699; ArgoL1; 1.
DR   Pfam; PF16488; ArgoL2; 1.
DR   Pfam; PF16487; ArgoMid; 1.
DR   Pfam; PF16486; ArgoN; 1.
DR   Pfam; PF02170; PAZ; 1.
DR   Pfam; PF02171; Piwi; 1.
DR   SMART; SM01163; DUF1785; 1.
DR   SMART; SM00949; PAZ; 1.
DR   SMART; SM00950; Piwi; 1.
DR   SUPFAM; SSF101690; SSF101690; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
DR   PROSITE; PS50821; PAZ; 1.
DR   PROSITE; PS50822; PIWI; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Developmental protein; Reference proteome;
KW   RNA-mediated gene silencing.
FT   CHAIN           1..979
FT                   /note="Protein argonaute PNH1"
FT                   /id="PRO_0000378443"
FT   DOMAIN          332..446
FT                   /note="PAZ"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00142"
FT   DOMAIN          620..941
FT                   /note="Piwi"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00150"
FT   REGION          1..95
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        408
FT                   /note="E -> Q (in Ref. 1; BAB96813)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        480..481
FT                   /note="TV -> S (in Ref. 1; BAB96813)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        522
FT                   /note="G -> V (in Ref. 1; BAB96813)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        818
FT                   /note="N -> I (in Ref. 1; BAB96813)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   979 AA;  108418 MW;  CB0C231A4D8DA159 CRC64;
     MLEVLDMAPP RHQPAAGKAG GGRGHGHGHG HGGGGGGPAA RKQPLQSSMA QPKAETAAAT
     AAVAPPEGGK KCGGGGGRRR GGRGRGGRAG AGPGPGLAAA PAVVVAPAAR AVIGPPVASK
     GLSFCRRPGF GTVGARCVVK ANHFLAELPD KDLTQYDVKI TPEVSSRSVN RAIMSELVRL
     YHDSDLGGRL PAYDGRKNLY TAGTLPFDAR EFVVRLTDDD DGTGVPPRER EYRVAIKFAA
     RADLHHLRQF IAGRQADAPQ EALQVLDIVL RELANRRYVS IGRSFYSPDI RKPQRLGDGL
     QSWCGFYQSI RPTQMGLSLN IDMSSTAFIE PLPVIEFVAQ ILGKDVISRP LSDANRIKIK
     KALRGVKVEV THRGNVRRKY RISGLTTQPT HELIFPIDDQ MNMKSVVEYF KEMYGFTIQH
     PHLPCLQVGN QKKANYLPME ACKIVEGQRY TKRLNEKQIT SLLKVTCRRP REQEMDILQT
     VQQNGYEQDP YAKEFGINIS EKLTSVEARV LPAPWLKYHD TGKEKECLPQ VGQWNMVNKK
     VINGCKVNHW ACINFSRSVQ ETTARGFCQE LAQMCQISGM EFNSEPVIPI YSARPDQVEK
     ALKHVYNMSL NKLKGKELEL LLAILPDNNG SLYGDIKRIC ETDLGLISQC CLTKHVFKIS
     KQYLANVSLK INVKMGGRNT VLLDAISWRI PLVSDIPTII FGADVTHPET GEDSSPSIAA
     VVASQDWPEV TKYAGLVCAQ AHRQELIQDL YKTWHDPQRG TVTGGMIREL LISFRKATGQ
     KPLRIIFYRD GVSEGQFYQV LLYELDAIRK ACASLEPNYQ PPVTFVVVQK RHHTRLFANN
     HKDRSSTDKS GNILPGTVVD SKICHPSEFD FYLCSHAGIQ GTSRPAHYHV LWDENNFTAD
     EMQTLTNNLC YTYARCTRSV SVVPPAYYAH LAAFRARFYM EPEMSENQTT SKSSTGTNGT
     SVKPLPAVKE KVKRVMFYC