ID   PNO1_ASHGO              Reviewed;         267 AA.
AC   Q753C6;
DT   20-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2013, sequence version 3.
DT   25-MAY-2022, entry version 87.
DE   RecName: Full=Pre-rRNA-processing protein PNO1;
GN   Name=PNO1; OrderedLocusNames=AFR390C;
OS   Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
OS   (Yeast) (Eremothecium gossypii).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Eremothecium.
OX   NCBI_TaxID=284811;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=15001715; DOI=10.1126/science.1095781;
RA   Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S.,
RA   Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A.,
RA   Gaffney T.D., Philippsen P.;
RT   "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces
RT   cerevisiae genome.";
RL   Science 304:304-307(2004).
RN   [2]
RP   GENOME REANNOTATION, AND SEQUENCE REVISION TO 205.
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=23749448; DOI=10.1534/g3.112.002881;
RA   Dietrich F.S., Voegeli S., Kuo S., Philippsen P.;
RT   "Genomes of Ashbya fungi isolated from insects reveal four mating-type
RT   loci, numerous translocations, lack of transposons, and distinct gene
RT   duplications.";
RL   G3 (Bethesda) 3:1225-1239(2013).
CC   -!- FUNCTION: Required for small ribosomal subunit (SSU) synthesis. Has a
CC       role in the processing of early nucleolar and late cytoplasmic pre-RNA
CC       species (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the small ribosomal subunit, ribosomal RNA
CC       processing complex (SSU RRP complex). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q99216}.
CC       Nucleus, nucleolus {ECO:0000250|UniProtKB:Q99216}.
CC   -!- SIMILARITY: Belongs to the PNO1 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAS53761.2; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AE016819; AAS53761.2; ALT_INIT; Genomic_DNA.
DR   RefSeq; NP_985937.2; NM_211292.2.
DR   AlphaFoldDB; Q753C6; -.
DR   SMR; Q753C6; -.
DR   STRING; 33169.AAS53761; -.
DR   GeneID; 4622209; -.
DR   KEGG; ago:AGOS_AFR390C; -.
DR   eggNOG; KOG3273; Eukaryota.
DR   InParanoid; Q753C6; -.
DR   Proteomes; UP000000591; Chromosome VI.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005730; C:nucleolus; IBA:GO_Central.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0042254; P:ribosome biogenesis; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.1370.10; -; 1.
DR   InterPro; IPR004087; KH_dom.
DR   InterPro; IPR036612; KH_dom_type_1_sf.
DR   InterPro; IPR039912; PNO1-like.
DR   PANTHER; PTHR12826:SF13; PTHR12826:SF13; 1.
DR   SMART; SM00322; KH; 1.
DR   SUPFAM; SSF54791; SSF54791; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Nucleus; Reference proteome; Ribosome biogenesis; RNA-binding.
FT   CHAIN           1..267
FT                   /note="Pre-rRNA-processing protein PNO1"
FT                   /id="PRO_0000278362"
FT   DOMAIN          188..240
FT                   /note="KH"
FT   REGION          1..35
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          43..62
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        9..29
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   267 AA;  29147 MW;  C0DB9924D66C23CC CRC64;
     MVAPTALKKS GTDGQTAPGP TGSRIVGTDN TRSIEDDDDI LIDQEDSGAA AAEQAEGARE
     QQGVVLDAEG KPRFAAAASV AETRVKAESR KVPVPPHRMT PLRNSWTKIY PPLVDHLKLQ
     VRMNLKTKSV ELRTHPRHTT DPGALQKGAD FIKAFTLGFD LDDSISLLRL DDLYIETFEI
     KDVKTLHGDH LSRAIGRIAG KDGKTKFAIE NATRTRIVLA DSKIHILGGF THIRMAREAV
     VSLILGSPPG KVYGNLRTVA SRLKERY