ID   PNO1_DANRE              Reviewed;         252 AA.
AC   Q6VEU3; Q6P0J4; Q6PHK2;
DT   09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2007, sequence version 2.
DT   03-AUG-2022, entry version 84.
DE   RecName: Full=RNA-binding protein PNO1;
GN   Name=pno1; ORFNames=zgc:65782;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=15497447; DOI=10.1080/10425170410001702159;
RA   Zhou G.-J., Zhang Y., Wang J., Guo J.H., Ni J., Zhong Z.-M., Wang L.-Q.,
RA   Dang Y.-J., Dai J.F., Yu L.;
RT   "Cloning and characterization of a novel human RNA binding protein gene
RT   PNO1.";
RL   DNA Seq. 15:219-224(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Positively regulates dimethylation of two adjacent adenosines
CC       in the loop of a conserved hairpin near the 3'-end of 18S rRNA.
CC       {ECO:0000250|UniProtKB:Q9NRX1}.
CC   -!- SUBCELLULAR LOCATION: Nucleus, nucleolus
CC       {ECO:0000250|UniProtKB:Q9NRX1}.
CC   -!- SIMILARITY: Belongs to the PNO1 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAH65596.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AY341884; AAQ24168.1; -; mRNA.
DR   EMBL; BC056522; AAH56522.1; -; mRNA.
DR   EMBL; BC065596; AAH65596.1; ALT_INIT; mRNA.
DR   RefSeq; NP_956842.2; NM_200548.1.
DR   AlphaFoldDB; Q6VEU3; -.
DR   SMR; Q6VEU3; -.
DR   STRING; 7955.ENSDARP00000004926; -.
DR   PaxDb; Q6VEU3; -.
DR   GeneID; 393520; -.
DR   KEGG; dre:393520; -.
DR   CTD; 56902; -.
DR   ZFIN; ZDB-GENE-040426-1419; pno1.
DR   eggNOG; KOG3273; Eukaryota.
DR   InParanoid; Q6VEU3; -.
DR   OrthoDB; 1269649at2759; -.
DR   PhylomeDB; Q6VEU3; -.
DR   Reactome; R-DRE-6791226; Major pathway of rRNA processing in the nucleolus and cytosol.
DR   PRO; PR:Q6VEU3; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Unplaced.
DR   GO; GO:0005730; C:nucleolus; ISS:UniProtKB.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.1370.10; -; 2.
DR   InterPro; IPR004087; KH_dom.
DR   InterPro; IPR036612; KH_dom_type_1_sf.
DR   InterPro; IPR039912; PNO1-like.
DR   PANTHER; PTHR12826:SF13; PTHR12826:SF13; 1.
DR   SMART; SM00322; KH; 1.
DR   SUPFAM; SSF54791; SSF54791; 1.
PE   2: Evidence at transcript level;
KW   Nucleus; Reference proteome; RNA-binding.
FT   CHAIN           1..252
FT                   /note="RNA-binding protein PNO1"
FT                   /id="PRO_0000270544"
FT   DOMAIN          168..225
FT                   /note="KH"
FT   REGION          1..72
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..15
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        21..43
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        31
FT                   /note="K -> R (in Ref. 1; AAQ24168)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        35
FT                   /note="C -> R (in Ref. 1; AAQ24168 and 2; AAH56522)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        242..245
FT                   /note="RAVA -> PGVG (in Ref. 1; AAQ24168)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   252 AA;  27884 MW;  5D3CA08B70F79310 CRC64;
     MESVSDSTAE VSATMDCDGN TEAFEKVKSK KREKCKRAAA EMDTQTEESS SSAPVKRPHF
     PALSGDQLGG GVDEMRKVPV PSHRYTPLKE NWLKIFTPIV ENLQLQVRFN LKTRQVEIKT
     CKETQDIGAL TRAADFVKAF VLGFQVEDAL ALIRLDELFL ETFDVTDVKP LKGDHLSRAI
     GRIAGKGGKT KFTIENVTKT RIVLADTKIH ILGSFQNIKM ARTAICNLIL GSPPSKVYGN
     LRAVATRSAE RF