ID   PNO1_DICDI              Reviewed;         239 AA.
AC   Q54K66;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   25-MAY-2022, entry version 90.
DE   RecName: Full=RNA-binding protein pno1;
GN   Name=pno1; ORFNames=DDB_G0287557;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: Positively regulates dimethylation of two adjacent adenosines
CC       in the loop of a conserved hairpin near the 3'-end of 18S rRNA.
CC       {ECO:0000250|UniProtKB:Q9NRX1}.
CC   -!- SUBCELLULAR LOCATION: Nucleus, nucleolus
CC       {ECO:0000250|UniProtKB:Q9NRX1}.
CC   -!- SIMILARITY: Belongs to the PNO1 family. {ECO:0000305}.
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DR   EMBL; AAFI02000102; EAL63678.1; -; Genomic_DNA.
DR   RefSeq; XP_637189.1; XM_632097.1.
DR   AlphaFoldDB; Q54K66; -.
DR   SMR; Q54K66; -.
DR   STRING; 44689.DDB0266405; -.
DR   PaxDb; Q54K66; -.
DR   EnsemblProtists; EAL63678; EAL63678; DDB_G0287557.
DR   GeneID; 8626191; -.
DR   KEGG; ddi:DDB_G0287557; -.
DR   dictyBase; DDB_G0287557; pno1.
DR   eggNOG; KOG3273; Eukaryota.
DR   HOGENOM; CLU_064992_1_0_1; -.
DR   InParanoid; Q54K66; -.
DR   OMA; IHIMGSF; -.
DR   PhylomeDB; Q54K66; -.
DR   Reactome; R-DDI-6791226; Major pathway of rRNA processing in the nucleolus and cytosol.
DR   PRO; PR:Q54K66; -.
DR   Proteomes; UP000002195; Chromosome 5.
DR   GO; GO:0005730; C:nucleolus; IBA:GO_Central.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.1370.10; -; 2.
DR   InterPro; IPR004087; KH_dom.
DR   InterPro; IPR036612; KH_dom_type_1_sf.
DR   InterPro; IPR039912; PNO1-like.
DR   PANTHER; PTHR12826:SF13; PTHR12826:SF13; 1.
DR   SMART; SM00322; KH; 1.
DR   SUPFAM; SSF54791; SSF54791; 1.
PE   3: Inferred from homology;
KW   Nucleus; Reference proteome; RNA-binding.
FT   CHAIN           1..239
FT                   /note="RNA-binding protein pno1"
FT                   /id="PRO_0000327681"
FT   DOMAIN          160..212
FT                   /note="KH"
FT   REGION          21..45
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        23..43
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   239 AA;  26954 MW;  F78F2E8E8EADDAE2 CRC64;
     MTEVIETKEV ENIISEQSLK TESVKVSNKR SRDDEEMKDS EGGVKKTSFP QISVEDTEEE
     QIRKVTIPFN RIAPLKANWQ QIYEPIVTHL KLQIRMNTKT RKVELKTSKS TKETSALQKA
     ADFVHAFSLG FEVNDAVAIL RLDDLYIDSF DVEDVKILKG DNLSRAIGRV AGKDGKTKFT
     IENVTKTRIV LADKRIHILG SYSNIRVAKD AICDLIIGSP PGKVYAKLRT VSSRIAERF