PNO1_HUMAN
ID PNO1_HUMAN Reviewed; 252 AA.
AC Q9NRX1; A8K6Q0; Q53G13; Q8WVB8;
DT 09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 154.
DE RecName: Full=RNA-binding protein PNO1;
DE AltName: Full=Partner of NOB1 {ECO:0000303|PubMed:15497447};
GN Name=PNO1;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=15497447; DOI=10.1080/10425170410001702159;
RA Zhou G.-J., Zhang Y., Wang J., Guo J.H., Ni J., Zhong Z.-M., Wang L.-Q.,
RA Dang Y.-J., Dai J.F., Yu L.;
RT "Cloning and characterization of a novel human RNA binding protein gene
RT PNO1.";
RL DNA Seq. 15:219-224(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Adrenal gland;
RX PubMed=10931946; DOI=10.1073/pnas.160270997;
RA Hu R.-M., Han Z.-G., Song H.-D., Peng Y.-D., Huang Q.-H., Ren S.-X.,
RA Gu Y.-J., Huang C.-H., Li Y.-B., Jiang C.-L., Fu G., Zhang Q.-H., Gu B.-W.,
RA Dai M., Mao Y.-F., Gao G.-F., Rong R., Ye M., Zhou J., Xu S.-H., Gu J.,
RA Shi J.-X., Jin W.-R., Zhang C.-K., Wu T.-M., Huang G.-Y., Chen Z.,
RA Chen M.-D., Chen J.-L.;
RT "Gene expression profiling in the human hypothalamus-pituitary-adrenal axis
RT and full-length cDNA cloning.";
RL Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT GLY-11.
RC TISSUE=Placenta;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15815621; DOI=10.1038/nature03466;
RA Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P.,
RA Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C.,
RA Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L.,
RA Du H., Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A.,
RA Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J.,
RA Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M.,
RA Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T.,
RA Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S.,
RA Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K.,
RA McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C.,
RA Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S.,
RA Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C.,
RA Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M.,
RA Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C.,
RA Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J.,
RA Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E.,
RA Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X.,
RA Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M.,
RA Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C.,
RA Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S.,
RA Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H.,
RA Wilson R.K.;
RT "Generation and annotation of the DNA sequences of human chromosomes 2 and
RT 4.";
RL Nature 434:724-731(2005).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND VARIANT GLY-11.
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Eye, and Lung;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 5-252, AND VARIANT GLY-11.
RA Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y.,
RA Tanaka A., Yokoyama S.;
RL Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
RN [8]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19413330; DOI=10.1021/ac9004309;
RA Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.;
RT "Lys-N and trypsin cover complementary parts of the phosphoproteome in a
RT refined SCX-based approach.";
RL Anal. Chem. 81:4493-4501(2009).
RN [9]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [10]
RP FUNCTION.
RX PubMed=25851604; DOI=10.1091/mbc.e15-02-0073;
RA Zorbas C., Nicolas E., Wacheul L., Huvelle E., Heurgue-Hamard V.,
RA Lafontaine D.L.;
RT "The human 18S rRNA base methyltransferases DIMT1L and WBSCR22-TRMT112 but
RT not rRNA modification are required for ribosome biogenesis.";
RL Mol. Biol. Cell 26:2080-2095(2015).
CC -!- FUNCTION: Positively regulates dimethylation of two adjacent adenosines
CC in the loop of a conserved hairpin near the 3'-end of 18S rRNA
CC (PubMed:25851604). {ECO:0000269|PubMed:25851604}.
CC -!- INTERACTION:
CC Q9NRX1; Q8WXH2: JPH3; NbExp=3; IntAct=EBI-712787, EBI-1055254;
CC Q9NRX1; Q9ULX3: NOB1; NbExp=4; IntAct=EBI-712787, EBI-373285;
CC Q9NRX1; Q9NRD5: PICK1; NbExp=3; IntAct=EBI-712787, EBI-79165;
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000269|PubMed:15497447}.
CC -!- TISSUE SPECIFICITY: Expressed in liver, lung, spleen and kidney. Weakly
CC expressed in thymus, testis and ovary. Weakly or not expressed in
CC heart, brain, skeletal muscle, placenta, pancreas, prostate, small
CC intestine, colon and peripheral blood leukocytes.
CC {ECO:0000269|PubMed:15497447}.
CC -!- SIMILARITY: Belongs to the PNO1 family. {ECO:0000305}.
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DR EMBL; AF349314; AAK29434.1; -; mRNA.
DR EMBL; AF164799; AAF80763.1; -; mRNA.
DR EMBL; AC017083; AAY14714.1; -; Genomic_DNA.
DR EMBL; CH471053; EAW99879.1; -; Genomic_DNA.
DR EMBL; BC008304; AAH08304.1; -; mRNA.
DR EMBL; BC018152; AAH18152.1; -; mRNA.
DR EMBL; AK223118; BAD96838.1; -; mRNA.
DR EMBL; AK291715; BAF84404.1; -; mRNA.
DR CCDS; CCDS1885.1; -.
DR RefSeq; NP_064528.1; NM_020143.3.
DR PDB; 6G18; EM; 3.60 A; x=1-252.
DR PDB; 6G4S; EM; 4.00 A; x=1-252.
DR PDB; 6G4W; EM; 4.50 A; x=1-252.
DR PDB; 6G51; EM; 4.10 A; x=1-252.
DR PDB; 6G53; EM; 4.50 A; x=1-252.
DR PDB; 6G5I; EM; 3.50 A; x=1-252.
DR PDB; 6ZUO; EM; 3.10 A; x=1-252.
DR PDB; 6ZXD; EM; 3.20 A; x=1-252.
DR PDB; 6ZXE; EM; 3.00 A; x=1-252.
DR PDB; 7MQ8; EM; 3.60 A; SW=1-252.
DR PDB; 7MQ9; EM; 3.87 A; SW=1-252.
DR PDB; 7MQA; EM; 2.70 A; SW=1-252.
DR PDBsum; 6G18; -.
DR PDBsum; 6G4S; -.
DR PDBsum; 6G4W; -.
DR PDBsum; 6G51; -.
DR PDBsum; 6G53; -.
DR PDBsum; 6G5I; -.
DR PDBsum; 6ZUO; -.
DR PDBsum; 6ZXD; -.
DR PDBsum; 6ZXE; -.
DR PDBsum; 7MQ8; -.
DR PDBsum; 7MQ9; -.
DR PDBsum; 7MQA; -.
DR AlphaFoldDB; Q9NRX1; -.
DR SMR; Q9NRX1; -.
DR BioGRID; 121232; 108.
DR IntAct; Q9NRX1; 41.
DR MINT; Q9NRX1; -.
DR STRING; 9606.ENSP00000263657; -.
DR GlyGen; Q9NRX1; 1 site, 1 O-linked glycan (1 site).
DR iPTMnet; Q9NRX1; -.
DR PhosphoSitePlus; Q9NRX1; -.
DR BioMuta; PNO1; -.
DR DMDM; 74734328; -.
DR EPD; Q9NRX1; -.
DR jPOST; Q9NRX1; -.
DR MassIVE; Q9NRX1; -.
DR MaxQB; Q9NRX1; -.
DR PaxDb; Q9NRX1; -.
DR PeptideAtlas; Q9NRX1; -.
DR PRIDE; Q9NRX1; -.
DR ProteomicsDB; 82432; -.
DR Antibodypedia; 30913; 88 antibodies from 17 providers.
DR DNASU; 56902; -.
DR Ensembl; ENST00000263657.7; ENSP00000263657.2; ENSG00000115946.8.
DR GeneID; 56902; -.
DR KEGG; hsa:56902; -.
DR MANE-Select; ENST00000263657.7; ENSP00000263657.2; NM_020143.4; NP_064528.1.
DR UCSC; uc002seh.5; human.
DR CTD; 56902; -.
DR DisGeNET; 56902; -.
DR GeneCards; PNO1; -.
DR HGNC; HGNC:32790; PNO1.
DR HPA; ENSG00000115946; Low tissue specificity.
DR MIM; 618710; gene.
DR neXtProt; NX_Q9NRX1; -.
DR OpenTargets; ENSG00000115946; -.
DR PharmGKB; PA162399854; -.
DR VEuPathDB; HostDB:ENSG00000115946; -.
DR eggNOG; KOG3273; Eukaryota.
DR GeneTree; ENSGT00390000018052; -.
DR HOGENOM; CLU_064992_2_0_1; -.
DR InParanoid; Q9NRX1; -.
DR OMA; IHIMGSF; -.
DR OrthoDB; 1269649at2759; -.
DR PhylomeDB; Q9NRX1; -.
DR TreeFam; TF300114; -.
DR PathwayCommons; Q9NRX1; -.
DR Reactome; R-HSA-6790901; rRNA modification in the nucleus and cytosol.
DR Reactome; R-HSA-6791226; Major pathway of rRNA processing in the nucleolus and cytosol.
DR SignaLink; Q9NRX1; -.
DR BioGRID-ORCS; 56902; 527 hits in 1050 CRISPR screens.
DR ChiTaRS; PNO1; human.
DR GeneWiki; PNO1; -.
DR GenomeRNAi; 56902; -.
DR Pharos; Q9NRX1; Tbio.
DR PRO; PR:Q9NRX1; -.
DR Proteomes; UP000005640; Chromosome 2.
DR RNAct; Q9NRX1; protein.
DR Bgee; ENSG00000115946; Expressed in buccal mucosa cell and 212 other tissues.
DR ExpressionAtlas; Q9NRX1; baseline and differential.
DR Genevisible; Q9NRX1; HS.
DR GO; GO:0005829; C:cytosol; TAS:Reactome.
DR GO; GO:0005730; C:nucleolus; IDA:UniProtKB.
DR GO; GO:0005654; C:nucleoplasm; IDA:HPA.
DR GO; GO:0003723; F:RNA binding; HDA:UniProtKB.
DR Gene3D; 3.30.1370.10; -; 2.
DR InterPro; IPR004087; KH_dom.
DR InterPro; IPR036612; KH_dom_type_1_sf.
DR InterPro; IPR039912; PNO1-like.
DR PANTHER; PTHR12826:SF13; PTHR12826:SF13; 1.
DR SMART; SM00322; KH; 1.
DR SUPFAM; SSF54791; SSF54791; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Nucleus; Reference proteome; RNA-binding.
FT CHAIN 1..252
FT /note="RNA-binding protein PNO1"
FT /id="PRO_0000270540"
FT DOMAIN 173..225
FT /note="KH"
FT REGION 1..71
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0007744|PubMed:19413330"
FT VARIANT 11
FT /note="R -> G (in dbSNP:rs2044693)"
FT /evidence="ECO:0000269|PubMed:14702039, ECO:0000269|Ref.5,
FT ECO:0000269|Ref.7"
FT /id="VAR_029814"
FT VARIANT 71
FT /note="G -> A (in dbSNP:rs7590838)"
FT /id="VAR_029815"
FT STRAND 75..79
FT /evidence="ECO:0007829|PDB:6ZXE"
FT HELIX 82..84
FT /evidence="ECO:0007829|PDB:6ZXE"
FT HELIX 86..90
FT /evidence="ECO:0007829|PDB:6ZXE"
FT HELIX 93..100
FT /evidence="ECO:0007829|PDB:6ZXE"
FT TURN 101..103
FT /evidence="ECO:0007829|PDB:6ZXE"
FT STRAND 105..110
FT /evidence="ECO:0007829|PDB:6ZXE"
FT TURN 111..114
FT /evidence="ECO:0007829|PDB:6ZXE"
FT STRAND 115..120
FT /evidence="ECO:0007829|PDB:6ZXE"
FT STRAND 126..129
FT /evidence="ECO:0007829|PDB:6ZXE"
FT HELIX 130..142
FT /evidence="ECO:0007829|PDB:6ZXE"
FT HELIX 146..149
FT /evidence="ECO:0007829|PDB:6ZXE"
FT HELIX 151..154
FT /evidence="ECO:0007829|PDB:6ZXE"
FT STRAND 159..164
FT /evidence="ECO:0007829|PDB:6ZXE"
FT HELIX 165..167
FT /evidence="ECO:0007829|PDB:6ZXE"
FT HELIX 174..184
FT /evidence="ECO:0007829|PDB:6ZXE"
FT HELIX 186..188
FT /evidence="ECO:0007829|PDB:6ZXE"
FT HELIX 189..198
FT /evidence="ECO:0007829|PDB:6ZXE"
FT STRAND 201..204
FT /evidence="ECO:0007829|PDB:6ZXE"
FT STRAND 206..213
FT /evidence="ECO:0007829|PDB:6ZXE"
FT HELIX 215..229
FT /evidence="ECO:0007829|PDB:6ZXE"
FT HELIX 234..246
FT /evidence="ECO:0007829|PDB:6ZXE"
SQ SEQUENCE 252 AA; 27924 MW; DDF2285D95D3D232 CRC64;
MESEMETQSA RAEEGFTQVT RKGGRRAKKR QAEQLSAAGE GGDAGRMDTE EARPAKRPVF
PPLCGDGLLS GKEETRKIPV PANRYTPLKE NWMKIFTPIV EHLGLQIRFN LKSRNVEIRT
CKETKDVSAL TKAADFVKAF ILGFQVEDAL ALIRLDDLFL ESFEITDVKP LKGDHLSRAI
GRIAGKGGKT KFTIENVTRT RIVLADVKVH ILGSFQNIKM ARTAICNLIL GNPPSKVYGN
IRAVASRSAD RF
