PNO1_MOUSE
ID PNO1_MOUSE Reviewed; 248 AA.
AC Q9CPS7;
DT 09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=RNA-binding protein PNO1;
GN Name=Pno1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=C57BL/6 X DBA;
RX PubMed=15497447; DOI=10.1080/10425170410001702159;
RA Zhou G.-J., Zhang Y., Wang J., Guo J.H., Ni J., Zhong Z.-M., Wang L.-Q.,
RA Dang Y.-J., Dai J.F., Yu L.;
RT "Cloning and characterization of a novel human RNA binding protein gene
RT PNO1.";
RL DNA Seq. 15:219-224(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J, and DBA/2J; TISSUE=Lung, Pancreas, and Tongue;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Czech II; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Positively regulates dimethylation of two adjacent adenosines
CC in the loop of a conserved hairpin near the 3'-end of 18S rRNA.
CC {ECO:0000250|UniProtKB:Q9NRX1}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus
CC {ECO:0000250|UniProtKB:Q9NRX1}.
CC -!- SIMILARITY: Belongs to the PNO1 family. {ECO:0000305}.
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DR EMBL; AF349950; AAK29665.1; -; mRNA.
DR EMBL; AK007324; BAB24959.1; -; mRNA.
DR EMBL; AK009146; BAB26104.1; -; mRNA.
DR EMBL; AK146152; BAE26936.1; -; mRNA.
DR EMBL; AK165942; BAE38474.1; -; mRNA.
DR EMBL; AK168177; BAE40137.1; -; mRNA.
DR EMBL; AL713926; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC020037; AAH20037.1; -; mRNA.
DR CCDS; CCDS24449.1; -.
DR RefSeq; NP_079719.1; NM_025443.2.
DR AlphaFoldDB; Q9CPS7; -.
DR SMR; Q9CPS7; -.
DR BioGRID; 211326; 1.
DR IntAct; Q9CPS7; 1.
DR MINT; Q9CPS7; -.
DR STRING; 10090.ENSMUSP00000020317; -.
DR iPTMnet; Q9CPS7; -.
DR PhosphoSitePlus; Q9CPS7; -.
DR EPD; Q9CPS7; -.
DR MaxQB; Q9CPS7; -.
DR PaxDb; Q9CPS7; -.
DR PeptideAtlas; Q9CPS7; -.
DR PRIDE; Q9CPS7; -.
DR ProteomicsDB; 289639; -.
DR Antibodypedia; 30913; 88 antibodies from 17 providers.
DR DNASU; 66249; -.
DR Ensembl; ENSMUST00000020317; ENSMUSP00000020317; ENSMUSG00000020116.
DR GeneID; 66249; -.
DR KEGG; mmu:66249; -.
DR UCSC; uc007icc.1; mouse.
DR CTD; 56902; -.
DR MGI; MGI:1913499; Pno1.
DR VEuPathDB; HostDB:ENSMUSG00000020116; -.
DR eggNOG; KOG3273; Eukaryota.
DR GeneTree; ENSGT00390000018052; -.
DR HOGENOM; CLU_064992_2_0_1; -.
DR InParanoid; Q9CPS7; -.
DR OMA; IHIMGSF; -.
DR OrthoDB; 1269649at2759; -.
DR PhylomeDB; Q9CPS7; -.
DR TreeFam; TF300114; -.
DR Reactome; R-MMU-6791226; Major pathway of rRNA processing in the nucleolus and cytosol.
DR BioGRID-ORCS; 66249; 25 hits in 78 CRISPR screens.
DR PRO; PR:Q9CPS7; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; Q9CPS7; protein.
DR Bgee; ENSMUSG00000020116; Expressed in cleaving embryo and 276 other tissues.
DR Genevisible; Q9CPS7; MM.
DR GO; GO:0005730; C:nucleolus; ISS:UniProtKB.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR Gene3D; 3.30.1370.10; -; 2.
DR InterPro; IPR004087; KH_dom.
DR InterPro; IPR036612; KH_dom_type_1_sf.
DR InterPro; IPR039912; PNO1-like.
DR PANTHER; PTHR12826:SF13; PTHR12826:SF13; 1.
DR SMART; SM00322; KH; 1.
DR SUPFAM; SSF54791; SSF54791; 1.
PE 1: Evidence at protein level;
KW Nucleus; Reference proteome; RNA-binding.
FT CHAIN 1..248
FT /note="RNA-binding protein PNO1"
FT /id="PRO_0000270541"
FT DOMAIN 169..221
FT /note="KH"
FT REGION 1..63
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 248 AA; 27454 MW; FBA75C3F79638251 CRC64;
METQSTGTED GFTPVTHRGG RRAKKRQAEQ SSAAGQDGEA GRMDTEEARP AKRPVFPPLS
GDQLLTGKEE TRKIPVPGNR YTPLKENWMK IFTPIVEHLG LQIRFNLKSR NVEIRTCKDT
KDVSALTKAA DFVKAFVLGF QVEDALALIR LDDLFLESFE ITDVKPLKGD HLSRAIGRIA
GKGGKTKFTI ENVTRTRIVL ADVHVHILGS FQNIKMARTA ICNLILGNPP SKVYGNIRAV
ASRSADRF
