ID   PNO1_SCHPO              Reviewed;         241 AA.
AC   O14044;
DT   20-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT   22-SEP-2009, sequence version 2.
DT   03-AUG-2022, entry version 126.
DE   RecName: Full=Pre-rRNA-processing protein pno1;
DE   AltName: Full=Ribosomal RNA-processing protein 28;
GN   Name=rbp28; Synonyms=pno1; ORFNames=SPAC2C4.11c;
OS   Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
OX   NCBI_TaxID=284812;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA   Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA   Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA   Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA   Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA   Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA   O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA   Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA   Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA   Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA   Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA   Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA   Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA   Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA   Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA   Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA   Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA   Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA   del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA   Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA   Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA   Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
RN   [2]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=15116432; DOI=10.1002/yea.1115;
RA   Chen X.Q., Du X., Liu J., Balasubramanian M.K., Balasundaram D.;
RT   "Identification of genes encoding putative nucleoporins and transport
RT   factors in the fission yeast Schizosaccharomyces pombe: a deletion
RT   analysis.";
RL   Yeast 21:495-509(2004).
RN   [3]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=16823372; DOI=10.1038/nbt1222;
RA   Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA   Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA   Yoshida M.;
RT   "ORFeome cloning and global analysis of protein localization in the fission
RT   yeast Schizosaccharomyces pombe.";
RL   Nat. Biotechnol. 24:841-847(2006).
RN   [4]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-52, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY.
RX   PubMed=18257517; DOI=10.1021/pr7006335;
RA   Wilson-Grady J.T., Villen J., Gygi S.P.;
RT   "Phosphoproteome analysis of fission yeast.";
RL   J. Proteome Res. 7:1088-1097(2008).
CC   -!- FUNCTION: Required for small ribosomal subunit (SSU) synthesis. Has a
CC       role in the processing of early nucleolar and late cytoplasmic pre-RNA
CC       species. {ECO:0000269|PubMed:15116432}.
CC   -!- SUBUNIT: Component of the small ribosomal subunit, ribosomal RNA
CC       processing complex (SSU RRP complex). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16823372}. Nucleus
CC       {ECO:0000269|PubMed:16823372}. Nucleus, nucleolus
CC       {ECO:0000269|PubMed:15116432}.
CC   -!- SIMILARITY: Belongs to the PNO1 family. {ECO:0000305}.
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DR   EMBL; CU329670; CAB16371.2; -; Genomic_DNA.
DR   PIR; T38522; T38522.
DR   RefSeq; NP_594514.2; NM_001019943.3.
DR   AlphaFoldDB; O14044; -.
DR   SMR; O14044; -.
DR   BioGRID; 278175; 3.
DR   STRING; 4896.SPAC2C4.11c.1; -.
DR   iPTMnet; O14044; -.
DR   MaxQB; O14044; -.
DR   PaxDb; O14044; -.
DR   PRIDE; O14044; -.
DR   EnsemblFungi; SPAC2C4.11c.1; SPAC2C4.11c.1:pep; SPAC2C4.11c.
DR   GeneID; 2541679; -.
DR   KEGG; spo:SPAC2C4.11c; -.
DR   PomBase; SPAC2C4.11c; rbp28.
DR   VEuPathDB; FungiDB:SPAC2C4.11c; -.
DR   eggNOG; KOG3273; Eukaryota.
DR   HOGENOM; CLU_064992_0_2_1; -.
DR   InParanoid; O14044; -.
DR   OMA; IHIMGSF; -.
DR   PhylomeDB; O14044; -.
DR   Reactome; R-SPO-6791226; Major pathway of rRNA processing in the nucleolus and cytosol.
DR   PRO; PR:O14044; -.
DR   Proteomes; UP000002485; Chromosome I.
DR   GO; GO:0005829; C:cytosol; HDA:PomBase.
DR   GO; GO:0005730; C:nucleolus; IDA:PomBase.
DR   GO; GO:0005634; C:nucleus; HDA:PomBase.
DR   GO; GO:0003723; F:RNA binding; ISO:PomBase.
DR   GO; GO:0006364; P:rRNA processing; ISO:PomBase.
DR   Gene3D; 3.30.1370.10; -; 1.
DR   InterPro; IPR004087; KH_dom.
DR   InterPro; IPR036612; KH_dom_type_1_sf.
DR   InterPro; IPR039912; PNO1-like.
DR   PANTHER; PTHR12826:SF13; PTHR12826:SF13; 1.
DR   SMART; SM00322; KH; 1.
DR   SUPFAM; SSF54791; SSF54791; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Nucleus; Phosphoprotein; Reference proteome;
KW   Ribosome biogenesis; RNA-binding.
FT   CHAIN           1..241
FT                   /note="Pre-rRNA-processing protein pno1"
FT                   /id="PRO_0000278359"
FT   DOMAIN          162..214
FT                   /note="KH"
FT   REGION          48..71
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         52
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0000269|PubMed:18257517"
SQ   SEQUENCE   241 AA;  26394 MW;  8BEFFBA0BFD3ACE2 CRC64;
     MLAPTAAVNV TNEGENDNVM IDTTRGEIEF SDSAVNGTMD IEGAPKFAPA KTSAEKKRGA
     KPQMRRVPIP PHRMTPLRNV WPKLYPPLVE HLLLQVRMNT KSRSVELRES KATKDPGALQ
     KGMDFVQAFA LGFDIDDAIA LLRLDDLYID TFEIKDVKTL QGDHLSRAIG RIAGQGGKTK
     FAIENASRTR IVLADSKIHI LGGFTNIRIA KDAVVSLILG SPPGKVYANL RNAAARAKER
     I