PNOC_MOUSE
ID PNOC_MOUSE Reviewed; 187 AA.
AC Q64387; Q61105; Q61938;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=Prepronociceptin;
DE AltName: Full=N23K/N27K;
DE Contains:
DE RecName: Full=Nocistatin;
DE Contains:
DE RecName: Full=Nociceptin;
DE AltName: Full=Orphanin FQ;
DE AltName: Full=PPNOC;
DE Contains:
DE RecName: Full=Orphanin FQ2;
DE Flags: Precursor;
GN Name=Pnoc; Synonyms=Npnc1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT).
RC TISSUE=Brain;
RX PubMed=8645247; DOI=10.1006/bbrc.1996.0300;
RA Houtani T., Nishi M., Takeshima H., Nukada T., Sugimoto T.;
RT "Structure and regional distribution of nociceptin/orphanin FQ precursor.";
RL Biochem. Biophys. Res. Commun. 219:714-719(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT).
RX PubMed=7503733; DOI=10.1006/bbrc.1995.2809;
RA Saito Y., Maruyama K., Saido T.C., Kawashima S.;
RT "N23K, a gene transiently up-regulated during neural differentiation,
RT encodes a precursor protein for a newly identified neuropeptide
RT nociceptin.";
RL Biochem. Biophys. Res. Commun. 217:539-545(1995).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM SHORT).
RC STRAIN=129/Sv;
RX PubMed=8710928; DOI=10.1073/pnas.93.16.8666;
RA Mollereau C., Simons M.-J., Soularue P., Liners F., Vassart G.,
RA Meunier J.-C., Parmentier M.;
RT "Structure, tissue distribution, and chromosomal localization of the
RT prepronociceptin gene.";
RL Proc. Natl. Acad. Sci. U.S.A. 93:8666-8670(1996).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG).
RX PubMed=8663129; DOI=10.1074/jbc.271.26.15615;
RA Saito Y., Maruyama K., Kawano H., Hagino-Yamagishi K., Kawamura K.,
RA Saido T.C., Kawashima S.;
RT "Molecular cloning and characterization of a novel form of neuropeptide
RT gene as a developmentally regulated molecule.";
RL J. Biol. Chem. 271:15615-15622(1996).
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 79-187.
RC TISSUE=Brain;
RX PubMed=8670093; DOI=10.1042/bj3150011;
RA Pan Y.-X., Xu J., Pasternak G.W.;
RT "Cloning and expression of a cDNA encoding a mouse brain orphanin
RT FQ/nociceptin precursor.";
RL Biochem. J. 315:11-13(1996).
RN [6]
RP FUNCTION OF ORPHANIN FQ2.
RX PubMed=9601687;
RA Rossi G.C., Mathis J.P., Pasternak G.W.;
RT "Analgesic activity of orphanin FQ2, murine prepro-orphanin FQ141-157 in
RT mice.";
RL NeuroReport 9:1165-1168(1998).
CC -!- FUNCTION: [Nociceptin]: Ligand of the opioid receptor-like receptor
CC OPRL1. It may act as a transmitter in the brain by modulating
CC nociceptive and locomotor behavior. May be involved in neuronal
CC differentiation and development. When administered
CC intracerebroventricularly, nociceptin induces hyperalgesia and
CC decreases locomotor activity.
CC -!- FUNCTION: [Nocistatin]: Blocks nociceptin action in pain transmission
CC by inhibiting nociceptin-induced hyperalgesia and allodynia.
CC {ECO:0000250|UniProtKB:O62647}.
CC -!- FUNCTION: [Orphanin FQ2]: Has potent analgesic activity.
CC {ECO:0000269|PubMed:9601687}.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=Short; Synonyms=N23K;
CC IsoId=Q64387-1; Sequence=Displayed;
CC Name=Long; Synonyms=N27K;
CC IsoId=Q64387-2; Sequence=VSP_001446;
CC -!- TISSUE SPECIFICITY: Brain and spinal cord. Low levels in kidney and
CC spleen.
CC -!- DEVELOPMENTAL STAGE: In embryonic brain, first detected at day 14 and
CC in postnatal brain, levels increase in day 1 and day 18. Levels
CC decrease significantly in adults.
CC -!- PTM: Specific enzymatic cleavages at paired basic residues probably
CC yield other active peptides besides nociceptin.
CC -!- PTM: The N-terminal domain contains 6 conserved cysteines thought to be
CC involved in disulfide bonding and/or processing.
CC -!- SIMILARITY: Belongs to the opioid neuropeptide precursor family.
CC {ECO:0000305}.
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DR EMBL; D82866; BAA11614.1; -; mRNA.
DR EMBL; D50056; BAA08774.1; -; mRNA.
DR EMBL; X97373; CAA66042.1; -; mRNA.
DR EMBL; X97371; CAA66041.1; -; Genomic_DNA.
DR EMBL; X97372; CAA66041.1; JOINED; Genomic_DNA.
DR EMBL; U44027; AAC52581.1; -; mRNA.
DR EMBL; D50055; BAA08773.1; -; mRNA.
DR CCDS; CCDS27213.1; -. [Q64387-1]
DR CCDS; CCDS88690.1; -. [Q64387-2]
DR PIR; JC4502; JC4502.
DR PIR; S70631; S70631.
DR RefSeq; NP_001192004.1; NM_001205075.1. [Q64387-2]
DR RefSeq; NP_035062.1; NM_010932.2. [Q64387-1]
DR RefSeq; XP_006518747.1; XM_006518684.2. [Q64387-2]
DR AlphaFoldDB; Q64387; -.
DR STRING; 10090.ENSMUSP00000054210; -.
DR iPTMnet; Q64387; -.
DR PhosphoSitePlus; Q64387; -.
DR MaxQB; Q64387; -.
DR PaxDb; Q64387; -.
DR PRIDE; Q64387; -.
DR ProteomicsDB; 289465; -. [Q64387-1]
DR ProteomicsDB; 289466; -. [Q64387-2]
DR Antibodypedia; 10388; 294 antibodies from 29 providers.
DR DNASU; 18155; -.
DR Ensembl; ENSMUST00000059339; ENSMUSP00000054210; ENSMUSG00000045731. [Q64387-1]
DR Ensembl; ENSMUST00000224594; ENSMUSP00000153589; ENSMUSG00000045731. [Q64387-2]
DR GeneID; 18155; -.
DR KEGG; mmu:18155; -.
DR UCSC; uc007uji.2; mouse. [Q64387-1]
DR CTD; 5368; -.
DR MGI; MGI:105308; Pnoc.
DR VEuPathDB; HostDB:ENSMUSG00000045731; -.
DR eggNOG; ENOG502S0DD; Eukaryota.
DR GeneTree; ENSGT00950000183149; -.
DR HOGENOM; CLU_143892_0_0_1; -.
DR InParanoid; Q64387; -.
DR OMA; WTPCTKI; -.
DR PhylomeDB; Q64387; -.
DR TreeFam; TF332620; -.
DR Reactome; R-MMU-375276; Peptide ligand-binding receptors.
DR Reactome; R-MMU-418594; G alpha (i) signalling events.
DR BioGRID-ORCS; 18155; 1 hit in 71 CRISPR screens.
DR PRO; PR:Q64387; -.
DR Proteomes; UP000000589; Chromosome 14.
DR RNAct; Q64387; protein.
DR Bgee; ENSMUSG00000045731; Expressed in lateral septal nucleus and 80 other tissues.
DR ExpressionAtlas; Q64387; baseline and differential.
DR Genevisible; Q64387; MM.
DR GO; GO:0043679; C:axon terminus; IBA:GO_Central.
DR GO; GO:0030425; C:dendrite; IBA:GO_Central.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0043025; C:neuronal cell body; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0001515; F:opioid peptide activity; IEA:UniProtKB-KW.
DR GO; GO:0031628; F:opioid receptor binding; IBA:GO_Central.
DR GO; GO:0007268; P:chemical synaptic transmission; ISO:MGI.
DR GO; GO:0007218; P:neuropeptide signaling pathway; ISO:MGI.
DR GO; GO:0007600; P:sensory perception; IBA:GO_Central.
DR InterPro; IPR002367; Nociceptin.
DR InterPro; IPR006024; Opioid_neupept.
DR PANTHER; PTHR11438; PTHR11438; 1.
DR Pfam; PF01160; Opiods_neuropep; 1.
DR PRINTS; PR01028; OPIOIDPRCRSR.
DR PRINTS; PR01031; ORPHNNPRCRSR.
DR PROSITE; PS01252; OPIOIDS_PRECURSOR; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cleavage on pair of basic residues; Disulfide bond;
KW Neuropeptide; Neurotransmitter; Opioid peptide; Reference proteome; Repeat;
KW Secreted; Signal.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT PROPEP 20..95
FT /id="PRO_0000008330"
FT PEPTIDE 98..138
FT /note="Nocistatin"
FT /evidence="ECO:0000305"
FT /id="PRO_0000008331"
FT PEPTIDE 141..157
FT /note="Nociceptin"
FT /evidence="ECO:0000250|UniProtKB:P55791"
FT /id="PRO_0000008332"
FT PEPTIDE 160..176
FT /note="Orphanin FQ2"
FT /evidence="ECO:0000305"
FT /id="PRO_0000008333"
FT PROPEP 180..187
FT /id="PRO_0000008334"
FT REPEAT 109..114
FT /note="1"
FT REPEAT 115..120
FT /note="2"
FT REPEAT 121..126
FT /note="3"
FT REGION 109..133
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 109..126
FT /note="3 X 6 AA tandem repeats of D-A-E-P-G-A"
FT VAR_SEQ 186..187
FT /note="NV -> IQVIPRTACVHSKTCRPGVRIPPSPRH (in isoform Long)"
FT /evidence="ECO:0000303|PubMed:8663129"
FT /id="VSP_001446"
FT CONFLICT 80
FT /note="V -> T (in Ref. 5; AAC52581)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 187 AA; 20884 MW; 81A8BFBEF2DCB913 CRC64;
MKILFCDVLL LSLLSSVFSS CPRDCLTCQE KLHPAPDSFN LKTCILQCEE KVFPRPLWTV
CTKVMASGSG QLSPADPELV SAALYQPKAS EMQHLKRMPR VRSLVQVRDA EPGADAEPGA
DAEPGADDAE EVEQKQLQKR FGGFTGARKS ARKLANQKRF SEFMRQYLVL SMQSSQRRRT
LHQNGNV
