PNOC_RAT
ID PNOC_RAT Reviewed; 181 AA.
AC Q62923; Q64162;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=Prepronociceptin;
DE Contains:
DE RecName: Full=Nocistatin;
DE Contains:
DE RecName: Full=Nociceptin;
DE AltName: Full=ORL1 receptor agonist;
DE AltName: Full=Orphanin FQ;
DE AltName: Full=PPNOC;
DE Contains:
DE RecName: Full=Orphanin FQ2;
DE Flags: Precursor;
GN Name=Pnoc;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC STRAIN=Sprague-Dawley;
RX PubMed=8710930; DOI=10.1073/pnas.93.16.8677;
RA Nothacker H.-P., Reinscheid R.K., Mansour A., Henningsen R.A., Ardati A.,
RA Monsma F.J. Jr., Watson S.J., Civelli O.;
RT "Primary structure and tissue distribution of the orphanin FQ precursor.";
RL Proc. Natl. Acad. Sci. U.S.A. 93:8677-8682(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RX PubMed=8710928; DOI=10.1073/pnas.93.16.8666;
RA Mollereau C., Simons M.-J., Soularue P., Liners F., Vassart G.,
RA Meunier J.-C., Parmentier M.;
RT "Structure, tissue distribution, and chromosomal localization of the
RT prepronociceptin gene.";
RL Proc. Natl. Acad. Sci. U.S.A. 93:8666-8670(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 13-181, PROTEIN SEQUENCE OF 135-151, AND
RP FUNCTION.
RC TISSUE=Brain;
RX PubMed=7566152; DOI=10.1038/377532a0;
RA Meunier J.-C., Mollereau C., Toll L., Suaudeau C., Moisand C.,
RA Alvinerie P., Butour J.-L., Guillemot J.-C., Ferrara P., Monsarrat B.,
RA Mazarguil H., Vassart G., Parmentier M., Costentin J.;
RT "Isolation and structure of the endogenous agonist of opioid receptor-like
RT ORL1 receptor.";
RL Nature 377:532-535(1995).
CC -!- FUNCTION: [Nociceptin]: Ligand of the opioid receptor-like receptor
CC OPRL1. It may act as a transmitter in the brain by modulating
CC nociceptive and locomotor behavior. May be involved in neuronal
CC differentiation and development. {ECO:0000269|PubMed:7566152}.
CC -!- FUNCTION: [Nocistatin]: Blocks nociceptin action in pain transmission
CC by inhibiting nociceptin-induced hyperalgesia and allodynia.
CC {ECO:0000250|UniProtKB:O62647}.
CC -!- FUNCTION: [Orphanin FQ2]: Has potent analgesic activity.
CC {ECO:0000250|UniProtKB:Q64387}.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- TISSUE SPECIFICITY: Expressed predominantly in the spinal cord and
CC brain, being more abundant in the hypothalamus and striatum. Also found
CC in small amounts in ovary. {ECO:0000269|PubMed:8710928,
CC ECO:0000269|PubMed:8710930}.
CC -!- PTM: Specific enzymatic cleavages at paired basic residues probably
CC yield other active peptides besides nociceptin.
CC -!- PTM: The N-terminal domain contains 6 conserved cysteines thought to be
CC involved in disulfide bonding and/or processing.
CC -!- SIMILARITY: Belongs to the opioid neuropeptide precursor family.
CC {ECO:0000305}.
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DR EMBL; U48262; AAC52726.1; -; mRNA.
DR EMBL; X97375; CAA66043.1; -; mRNA.
DR EMBL; S79730; AAB35376.1; -; mRNA.
DR PIR; JC6151; JC6151.
DR RefSeq; NP_037139.1; NM_013007.1.
DR RefSeq; XP_006252261.1; XM_006252199.3.
DR AlphaFoldDB; Q62923; -.
DR SMR; Q62923; -.
DR STRING; 10116.ENSRNOP00000019117; -.
DR PaxDb; Q62923; -.
DR Ensembl; ENSRNOT00000019117; ENSRNOP00000019117; ENSRNOG00000014231.
DR GeneID; 25516; -.
DR KEGG; rno:25516; -.
DR UCSC; RGD:3362; rat.
DR CTD; 5368; -.
DR RGD; 3362; Pnoc.
DR eggNOG; ENOG502S0DD; Eukaryota.
DR GeneTree; ENSGT00950000183149; -.
DR HOGENOM; CLU_143892_0_0_1; -.
DR InParanoid; Q62923; -.
DR OMA; WTPCTKI; -.
DR OrthoDB; 1486887at2759; -.
DR PhylomeDB; Q62923; -.
DR Reactome; R-RNO-375276; Peptide ligand-binding receptors.
DR Reactome; R-RNO-418594; G alpha (i) signalling events.
DR PRO; PR:Q62923; -.
DR Proteomes; UP000002494; Chromosome 15.
DR Bgee; ENSRNOG00000014231; Expressed in cerebellum and 6 other tissues.
DR Genevisible; Q62923; RN.
DR GO; GO:0043679; C:axon terminus; IBA:GO_Central.
DR GO; GO:0030425; C:dendrite; IBA:GO_Central.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0043025; C:neuronal cell body; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0001515; F:opioid peptide activity; IEA:UniProtKB-KW.
DR GO; GO:0031628; F:opioid receptor binding; IBA:GO_Central.
DR GO; GO:0007268; P:chemical synaptic transmission; IDA:RGD.
DR GO; GO:0007565; P:female pregnancy; IEP:RGD.
DR GO; GO:0007218; P:neuropeptide signaling pathway; IDA:RGD.
DR GO; GO:0007600; P:sensory perception; IBA:GO_Central.
DR InterPro; IPR002367; Nociceptin.
DR InterPro; IPR006024; Opioid_neupept.
DR PANTHER; PTHR11438; PTHR11438; 1.
DR Pfam; PF01160; Opiods_neuropep; 1.
DR PRINTS; PR01028; OPIOIDPRCRSR.
DR PRINTS; PR01031; ORPHNNPRCRSR.
DR PROSITE; PS01252; OPIOIDS_PRECURSOR; 1.
PE 1: Evidence at protein level;
KW Cleavage on pair of basic residues; Direct protein sequencing;
KW Disulfide bond; Neuropeptide; Neurotransmitter; Opioid peptide;
KW Reference proteome; Repeat; Secreted; Signal.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT PROPEP 20..95
FT /id="PRO_0000008335"
FT PEPTIDE 98..132
FT /note="Nocistatin"
FT /evidence="ECO:0000305"
FT /id="PRO_0000008336"
FT PEPTIDE 135..151
FT /note="Nociceptin"
FT /evidence="ECO:0000269|PubMed:7566152"
FT /id="PRO_0000008337"
FT PEPTIDE 154..170
FT /note="Orphanin FQ2"
FT /evidence="ECO:0000305"
FT /id="PRO_0000008338"
FT PROPEP 174..181
FT /id="PRO_0000008339"
FT REPEAT 109..114
FT /note="1"
FT REPEAT 115..120
FT /note="2"
FT REGION 103..125
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 109..120
FT /note="2 X 6 AA tandem repeats of D-A-E-P-X-A"
SQ SEQUENCE 181 AA; 20479 MW; B2E9119CF8702C37 CRC64;
MKILFCDVLL LSLLSSVFSS CPEDCLTCQE RLHPAPGSFN LKLCILQCEE KVFPRPLWTL
CTKAMASDSE QLSPADPELT SAALYQSKAS EMQHLKRMPR VRSVVQARDA EPEADAEPVA
DEADEVEQKQ LQKRFGGFTG ARKSARKLAN QKRFSEFMRQ YLVLSMQSSQ RRRTLHQNGN
V