ID   PNPA_PSEWB              Reviewed;         403 AA.
AC   C1I201;
DT   26-JUN-2013, integrated into UniProtKB/Swiss-Prot.
DT   26-MAY-2009, sequence version 1.
DT   03-AUG-2022, entry version 42.
DE   RecName: Full=Para-nitrophenol 4-monooxygenase;
DE            EC=1.14.13.167;
GN   Name=pnpA;
OS   Pseudomonas sp. (strain WBC-3).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=165468;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, DISRUPTION
RP   PHENOTYPE, BIOPHYSICOCHEMICAL PROPERTIES, AND SUBUNIT.
RX   PubMed=19218392; DOI=10.1128/jb.01566-08;
RA   Zhang J.J., Liu H., Xiao Y., Zhang X.E., Zhou N.Y.;
RT   "Identification and characterization of catabolic para-nitrophenol 4-
RT   monooxygenase and para-benzoquinone reductase from Pseudomonas sp. strain
RT   WBC-3.";
RL   J. Bacteriol. 191:2703-2710(2009).
CC   -!- FUNCTION: Involved in the degradation of para-nitrophenol (4-NP).
CC       Catalyzes oxidation of 4-nitrophenol (4-NP) at position 4 with
CC       concomitant removal of the nitro group as nitrite and production of
CC       para-benzoquinone. {ECO:0000269|PubMed:19218392}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4-nitrophenol + H(+) + NADPH + O2 = 1,4-benzoquinone + H2O +
CC         NADP(+) + nitrite; Xref=Rhea:RHEA:34327, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:16301,
CC         ChEBI:CHEBI:16509, ChEBI:CHEBI:57783, ChEBI:CHEBI:57917,
CC         ChEBI:CHEBI:58349; EC=1.14.13.167;
CC         Evidence={ECO:0000269|PubMed:19218392};
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000250};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=12 uM for 4-NP {ECO:0000269|PubMed:19218392};
CC         KM=137.4 uM for NADP {ECO:0000269|PubMed:19218392};
CC   -!- PATHWAY: Xenobiotic degradation; 4-nitrophenol degradation.
CC   -!- SUBUNIT: Monomer. {ECO:0000269|PubMed:19218392}.
CC   -!- DISRUPTION PHENOTYPE: Cells lacking this gene are not able to grow on
CC       4-NP. {ECO:0000269|PubMed:19218392}.
CC   -!- SIMILARITY: Belongs to the PheA/TfdB FAD monooxygenase family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; EF577044; ABU50908.1; -; Genomic_DNA.
DR   AlphaFoldDB; C1I201; -.
DR   SMR; C1I201; -.
DR   KEGG; ag:ABU50908; -.
DR   BioCyc; MetaCyc:MON-13030; -.
DR   BRENDA; 1.14.13.166; 5085.
DR   BRENDA; 1.14.13.167; 5085.
DR   SABIO-RK; C1I201; -.
DR   UniPathway; UPA01030; -.
DR   GO; GO:0018632; F:4-nitrophenol 4-monooxygenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0071949; F:FAD binding; IEA:InterPro.
DR   GO; GO:0004497; F:monooxygenase activity; IDA:UniProtKB.
DR   GO; GO:0046196; P:4-nitrophenol catabolic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0019439; P:aromatic compound catabolic process; IDA:UniProtKB.
DR   Gene3D; 3.50.50.60; -; 1.
DR   InterPro; IPR002938; FAD-bd.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   Pfam; PF01494; FAD_binding_3; 1.
DR   SUPFAM; SSF51905; SSF51905; 1.
PE   1: Evidence at protein level;
KW   Aromatic hydrocarbons catabolism; FAD; Flavoprotein; Monooxygenase; NADP;
KW   Oxidoreductase.
FT   CHAIN           1..403
FT                   /note="Para-nitrophenol 4-monooxygenase"
FT                   /id="PRO_0000422666"
FT   BINDING         6..35
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000250"
FT   BINDING         279..289
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   403 AA;  44867 MW;  45F863B5167FC990 CRC64;
     METLDGVVVV GGGPVGLLTA LKLGKAGIKV VVLEAEPGVS PSPRAVAYMP PTAAALDRFG
     LLQDIRKRAV MCPDFAYRHG NGELIAKMDW SVLSQDTQYP YMLLLGQNHV SNVIFQHLRE
     LPNVEIRWNH RVEEVDQDDA YVTIETSSPG GTSRLRARWL AATDGARSTV RQKIGLTFDG
     ITWDERLVAT NVFYDFSLHG YSRANFVHDP VDWAVVVQLD KTGLWRVCYG EDASLSDAEV
     RRRLPERFKR LLPGAPTPDQ YRVDHLNPYR VHQRCAAEFR RGRVVLAGDA AHATNPMGGL
     GLSGGVLDAE HLAEALIAVI KNGASTKTLD EYSIDRRKVF LEFTSPTATA NFTWMKESDP
     AQRIRDDAMF KEAGTDRAVM RQFLLDLEKL NGRRVIEKKL KAA