AT1B3_RAT
ID AT1B3_RAT Reviewed; 279 AA.
AC Q63377;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=Sodium/potassium-transporting ATPase subunit beta-3;
DE AltName: Full=Sodium/potassium-dependent ATPase subunit beta-3;
DE Short=ATPB-3;
DE AltName: CD_antigen=CD298;
GN Name=Atp1b3;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Brain;
RA Watanabe T., Sato M., Yoshida T., Suzuki Y.;
RL Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Prostate;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP SUBCELLULAR LOCATION, INTERACTION WITH ATP12A, TISSUE SPECIFICITY, AND
RP INDUCTION BY DIET.
RX PubMed=9950762; DOI=10.1152/ajpcell.1999.276.2.c350;
RA Sangan P., Kolla S.S., Rajendran V.M., Kashgarian M., Binder H.J.;
RT "Colonic H-K-ATPase beta-subunit: identification in apical membranes and
RT regulation by dietary K depletion.";
RL Am. J. Physiol. 276:C350-C360(1999).
RN [4]
RP TISSUE SPECIFICITY, AND SUBCELLULAR LOCATION.
RX PubMed=14749213; DOI=10.1152/ajpcell.00393.2003;
RA Pestov N.B., Korneenko T.V., Radkov R., Zhao H., Shakhparonov M.I.,
RA Modyanov N.N.;
RT "Identification of the beta-subunit for nongastric H-K-ATPase in rat
RT anterior prostate.";
RL Am. J. Physiol. 286:C1229-C1237(2004).
CC -!- FUNCTION: This is the non-catalytic component of the active enzyme,
CC which catalyzes the hydrolysis of ATP coupled with the exchange of
CC Na(+) and K(+) ions across the plasma membrane. The exact function of
CC the beta-3 subunit is not known.
CC -!- SUBUNIT: The sodium/potassium-transporting ATPase is composed of a
CC catalytic alpha subunit, an auxiliary non-catalytic beta subunit and an
CC additional regulatory subunit. Interacts with catalytic alpha subunit
CC ATP12A. {ECO:0000269|PubMed:9950762}.
CC -!- SUBCELLULAR LOCATION: Apical cell membrane
CC {ECO:0000269|PubMed:9950762}; Single-pass type II membrane protein
CC {ECO:0000255}. Basolateral cell membrane {ECO:0000269|PubMed:14749213,
CC ECO:0000269|PubMed:9950762}; Single-pass type II membrane protein
CC {ECO:0000255}. Melanosome {ECO:0000250|UniProtKB:P54709}.
CC Note=Identified by mass spectrometry in melanosome fractions from stage
CC I to stage IV. {ECO:0000250|UniProtKB:P54709}.
CC -!- TISSUE SPECIFICITY: Expressed in distal colon, proximal colon, ileum,
CC jejunum, stomach, liver, lung, kidney, spleen, and heart
CC (PubMed:9950762). Expressed in the brain, testes and anterior prostate
CC (at protein level) (PubMed:14749213). {ECO:0000269|PubMed:14749213,
CC ECO:0000269|PubMed:9950762}.
CC -!- INDUCTION: Up-regulated in distal colon in response to K(+) free diet.
CC {ECO:0000269|PubMed:9950762}.
CC -!- DOMAIN: The C-terminal lobe folds into an immunoglobulin-like domain
CC and may mediate cell adhesion properties. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the X(+)/potassium ATPases subunit beta family.
CC {ECO:0000305}.
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DR EMBL; D84450; BAA12668.1; -; mRNA.
DR EMBL; BC061719; AAH61719.1; -; mRNA.
DR RefSeq; NP_037045.1; NM_012913.1.
DR AlphaFoldDB; Q63377; -.
DR SMR; Q63377; -.
DR BioGRID; 247428; 1.
DR IntAct; Q63377; 2.
DR STRING; 10116.ENSRNOP00000015475; -.
DR BindingDB; Q63377; -.
DR ChEMBL; CHEMBL4106166; -.
DR GlyGen; Q63377; 2 sites.
DR SwissPalm; Q63377; -.
DR jPOST; Q63377; -.
DR PaxDb; Q63377; -.
DR PRIDE; Q63377; -.
DR Ensembl; ENSRNOT00000015476; ENSRNOP00000015475; ENSRNOG00000011501.
DR GeneID; 25390; -.
DR KEGG; rno:25390; -.
DR UCSC; RGD:2172; rat.
DR CTD; 483; -.
DR RGD; 2172; Atp1b3.
DR eggNOG; KOG3927; Eukaryota.
DR GeneTree; ENSGT01030000234579; -.
DR HOGENOM; CLU_057702_1_1_1; -.
DR InParanoid; Q63377; -.
DR OMA; PKLGKWE; -.
DR OrthoDB; 998086at2759; -.
DR PhylomeDB; Q63377; -.
DR TreeFam; TF314618; -.
DR Reactome; R-RNO-210991; Basigin interactions.
DR Reactome; R-RNO-5578775; Ion homeostasis.
DR Reactome; R-RNO-936837; Ion transport by P-type ATPases.
DR SABIO-RK; Q63377; -.
DR PRO; PR:Q63377; -.
DR Proteomes; UP000002494; Chromosome 8.
DR Bgee; ENSRNOG00000011501; Expressed in lung and 20 other tissues.
DR Genevisible; Q63377; RN.
DR GO; GO:0016324; C:apical plasma membrane; IDA:UniProtKB.
DR GO; GO:0016323; C:basolateral plasma membrane; IDA:UniProtKB.
DR GO; GO:0005901; C:caveola; IDA:RGD.
DR GO; GO:0005737; C:cytoplasm; ISO:RGD.
DR GO; GO:0042470; C:melanosome; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; IDA:RGD.
DR GO; GO:0005890; C:sodium:potassium-exchanging ATPase complex; IDA:RGD.
DR GO; GO:0036126; C:sperm flagellum; ISO:RGD.
DR GO; GO:0001671; F:ATPase activator activity; ISO:RGD.
DR GO; GO:0051117; F:ATPase binding; ISO:RGD.
DR GO; GO:0005391; F:P-type sodium:potassium-exchanging transporter activity; IDA:RGD.
DR GO; GO:0030674; F:protein-macromolecule adaptor activity; ISO:RGD.
DR GO; GO:0030007; P:cellular potassium ion homeostasis; ISO:RGD.
DR GO; GO:0006883; P:cellular sodium ion homeostasis; ISO:RGD.
DR GO; GO:0086009; P:membrane repolarization; ISO:RGD.
DR GO; GO:0030001; P:metal ion transport; IDA:RGD.
DR GO; GO:0032781; P:positive regulation of ATP-dependent activity; ISO:RGD.
DR GO; GO:1903288; P:positive regulation of potassium ion import across plasma membrane; ISO:RGD.
DR GO; GO:1901018; P:positive regulation of potassium ion transmembrane transporter activity; ISO:RGD.
DR GO; GO:1903278; P:positive regulation of sodium ion export across plasma membrane; ISO:RGD.
DR GO; GO:1990573; P:potassium ion import across plasma membrane; ISO:RGD.
DR GO; GO:0071805; P:potassium ion transmembrane transport; ISO:RGD.
DR GO; GO:0006813; P:potassium ion transport; IDA:RGD.
DR GO; GO:0072659; P:protein localization to plasma membrane; ISO:RGD.
DR GO; GO:0050821; P:protein stabilization; ISO:RGD.
DR GO; GO:0036376; P:sodium ion export across plasma membrane; ISO:RGD.
DR GO; GO:0035725; P:sodium ion transmembrane transport; ISO:RGD.
DR GO; GO:0006814; P:sodium ion transport; IDA:RGD.
DR Gene3D; 2.60.40.1660; -; 1.
DR InterPro; IPR000402; Na/K_ATPase_sub_beta.
DR InterPro; IPR038702; Na/K_ATPase_sub_beta_sf.
DR PANTHER; PTHR11523; PTHR11523; 1.
DR Pfam; PF00287; Na_K-ATPase; 1.
DR TIGRFAMs; TIGR01107; Na_K_ATPase_bet; 1.
DR PROSITE; PS00390; ATPASE_NA_K_BETA_1; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Disulfide bond; Glycoprotein; Ion transport; Membrane;
KW Potassium; Potassium transport; Reference proteome; Signal-anchor; Sodium;
KW Sodium transport; Sodium/potassium transport; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..279
FT /note="Sodium/potassium-transporting ATPase subunit beta-3"
FT /id="PRO_0000219110"
FT TOPO_DOM 1..35
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 36..56
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 57..279
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT REGION 186..279
FT /note="immunoglobulin-like"
FT /evidence="ECO:0000250"
FT CARBOHYD 124
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 158
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 128..144
FT /evidence="ECO:0000250"
FT DISULFID 154..170
FT /evidence="ECO:0000250"
FT DISULFID 191..250
FT /evidence="ECO:0000250"
SQ SEQUENCE 279 AA; 31830 MW; 45DD461CFE2A6E1E CRC64;
MTKTEKKSFH QSLAEWKLFI YNPTSGEFLG RTSKSWGLIL LFYLVFYGFL AALFTFTMWV
MLQTLNDEVP KYRDQIPSPG LMVFPKPPTA LDYTYSMSDP HTYKKFVEDL KNFLKPYSVE
EQKNLTDCPG GALFHQEGPD YSACQFPVSL LQECSGVNDS NFGYSKGQPC VLVKMNRIIE
LVPDGAPYIT CITKEENIAN IVTYPDDGLI DLKYFPYYGK KRHVGYRQPL VAVQVIFGAD
ATKKEVTIEC QIDGTRNLKN KNERDKFLGR VSFKVIAHA
