ID   AT1_PAPSO               Reviewed;         471 AA.
AC   I3PLR4;
DT   03-JUL-2019, integrated into UniProtKB/Swiss-Prot.
DT   05-SEP-2012, sequence version 1.
DT   03-AUG-2022, entry version 22.
DE   RecName: Full=(13S,14R)-1,13-dihydroxy-N-methylcanadine 13-O-acetyltransferase AT1 {ECO:0000305};
DE            EC=2.3.1.285 {ECO:0000269|PubMed:25485687, ECO:0000269|PubMed:27378283};
DE   AltName: Full=Acetyltransferase 1 {ECO:0000303|PubMed:22653730};
DE            Short=PsAT1 {ECO:0000303|PubMed:27378283};
GN   Name=AT1 {ECO:0000303|PubMed:22653730};
OS   Papaver somniferum (Opium poppy).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Ranunculales; Papaveraceae; Papaveroideae;
OC   Papaver.
OX   NCBI_TaxID=3469;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=22653730; DOI=10.1126/science.1220757;
RA   Winzer T., Gazda V., He Z., Kaminski F., Kern M., Larson T.R., Li Y.,
RA   Meade F., Teodor R., Vaistij F.E., Walker C., Bowser T.A., Graham I.A.;
RT   "A Papaver somniferum 10-gene cluster for synthesis of the anticancer
RT   alkaloid noscapine.";
RL   Science 336:1704-1708(2012).
RN   [2]
RP   FUNCTION, CATALYTIC ACTIVITY, AND BIOPHYSICOCHEMICAL PROPERTIES.
RX   PubMed=25485687; DOI=10.1038/nchembio.1717;
RA   Dang T.T., Chen X., Facchini P.J.;
RT   "Acetylation serves as a protective group in noscapine biosynthesis in
RT   opium poppy.";
RL   Nat. Chem. Biol. 11:104-106(2015).
RN   [3]
RP   FUNCTION, AND CATALYTIC ACTIVITY.
RX   PubMed=27378283; DOI=10.1038/ncomms12137;
RA   Li Y., Smolke C.D.;
RT   "Engineering biosynthesis of the anticancer alkaloid noscapine in yeast.";
RL   Nat. Commun. 7:12137-12137(2016).
RN   [4]
RP   FUNCTION.
RX   PubMed=29610307; DOI=10.1073/pnas.1721469115;
RA   Li Y., Li S., Thodey K., Trenchard I., Cravens A., Smolke C.D.;
RT   "Complete biosynthesis of noscapine and halogenated alkaloids in yeast.";
RL   Proc. Natl. Acad. Sci. U.S.A. 115:E3922-E3931(2018).
CC   -!- FUNCTION: Acetyltransferase involved in the biosynthesis of the
CC       benzylisoquinoline alkaloid noscapine (PubMed:25485687,
CC       PubMed:27378283, PubMed:29610307). Converts (13S,14R)-1,13-dihydroxy-N-
CC       methylcanadine to (13S,14R)-13-O-acetyl-1-hydroxy-N-methylcanadine
CC       (PubMed:25485687, PubMed:27378283). {ECO:0000269|PubMed:25485687,
CC       ECO:0000269|PubMed:27378283, ECO:0000269|PubMed:29610307}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(13S,14R)-1,13-dihydroxy-N-methylcanadine + acetyl-CoA =
CC         (13S,14R)-13-O-acetyl-1-hydroxy-N-methylcanadine + CoA;
CC         Xref=Rhea:RHEA:57384, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288,
CC         ChEBI:CHEBI:141639, ChEBI:CHEBI:141640; EC=2.3.1.285;
CC         Evidence={ECO:0000269|PubMed:25485687, ECO:0000269|PubMed:27378283};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:57385;
CC         Evidence={ECO:0000305|PubMed:25485687};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=16.5 uM for (13S,14R)-1,13-dihydroxy-N-methylcanadine
CC         {ECO:0000269|PubMed:25485687};
CC         KM=35.3 uM for acetyl-CoA {ECO:0000269|PubMed:25485687};
CC         Vmax=152 nmol/min/mg enzyme with (13S,14R)-1,13-dihydroxy-N-
CC         methylcanadine as substrate {ECO:0000269|PubMed:25485687};
CC         Vmax=324 nmol/min/mg enzyme with acetyl-CoA as substrate
CC         {ECO:0000269|PubMed:25485687};
CC   -!- PATHWAY: Alkaloid biosynthesis. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the plant acyltransferase family. {ECO:0000305}.
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DR   EMBL; JQ659008; AFB74620.1; -; Genomic_DNA.
DR   AlphaFoldDB; I3PLR4; -.
DR   SMR; I3PLR4; -.
DR   EnsemblPlants; RZC84731; RZC84731; C5167_047517.
DR   Gramene; RZC84731; RZC84731; C5167_047517.
DR   KEGG; ag:AFB74620; -.
DR   BioCyc; MetaCyc:MON-20624; -.
DR   BRENDA; 2.3.1.285; 4515.
DR   GO; GO:0016746; F:acyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0009820; P:alkaloid metabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.559.10; -; 2.
DR   InterPro; IPR023213; CAT-like_dom_sf.
PE   1: Evidence at protein level;
KW   Acyltransferase; Alkaloid metabolism; Transferase.
FT   CHAIN           1..471
FT                   /note="(13S,14R)-1,13-dihydroxy-N-methylcanadine 13-O-
FT                   acetyltransferase AT1"
FT                   /id="PRO_0000447600"
SQ   SEQUENCE   471 AA;  52494 MW;  24FD2F411E5EECFC CRC64;
     MATMSSAAVE VISKETIKPR NPTPYQLRNY NMSLLDQYSS LVYVPIILFY PAASDANSTG
     SKHHDDLHLL KRSLSETLVH FYPMAGRMKD NMTVDCNDEG IDFFEVRIKG RMCDFMMKSD
     AHLSLLLPSE VASTNFVKEA QVIVQVNMFD CGGTAICFCI SNKIADACTM ITFIRSLAGT
     TNIARRGSSI AAPTTNQNLV PSFDSTSLFP PSEQLASQVS YPTQDSTSVD KLVSKRFVFD
     AAKITSAREK LQSLMHDKYK CHRPTRVEVV SALIWKSAVK SAPPGSISTV THAMNFRKKM
     DPPLQDASFG NLCVVVTAVL PATTATTTNP ATKKVSSTSN EEQVALDELS DFVALLRREI
     DKVKGDKGCM EKIIQKFIYG HDASVAKDSD VEDKVTALFM TSWCKFGFYE ADFGWGTPVW
     VTTVPLIEPK YKNMVFMNDM KCGEGIEVWV NFLEDDMTKF EHHLREILQL F