AT1_TOXGM
ID AT1_TOXGM Reviewed; 534 AA.
AC S8EZA7;
DT 23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT 16-OCT-2013, sequence version 1.
DT 03-AUG-2022, entry version 37.
DE RecName: Full=Arginine transporter 1 {ECO:0000305};
DE AltName: Full=Amino acid transporter 1 {ECO:0000303|PubMed:30742695};
DE AltName: Full=Apicomplexan amino acid transporter 1 {ECO:0000303|PubMed:30742695};
DE Short=TgApiAT1 {ECO:0000303|PubMed:30742695};
DE AltName: Full=Major facilitator superfamily domain-containing protein {ECO:0000305|PubMed:28205520};
DE AltName: Full=Novel putative transporter 1 {ECO:0000303|PubMed:28205520};
DE Short=TgNPT1 {ECO:0000303|PubMed:28205520};
GN Name=ApiAT1 {ECO:0000303|PubMed:30742695};
GN Synonyms=NPT1 {ECO:0000303|PubMed:28205520};
GN ORFNames=TGME49_215490 {ECO:0000312|EMBL:EPT26443.1};
OS Toxoplasma gondii (strain ATCC 50611 / Me49).
OC Eukaryota; Sar; Alveolata; Apicomplexa; Conoidasida; Coccidia;
OC Eucoccidiorida; Eimeriorina; Sarcocystidae; Toxoplasma.
OX NCBI_TaxID=508771 {ECO:0000312|Proteomes:UP000001529};
RN [1] {ECO:0000312|Proteomes:UP000001529}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 50611 / Me49 {ECO:0000312|Proteomes:UP000001529};
RA Sibley D., Venepally P., Karamycheva S., Hadjithomas M., Khan A., Brunk B.,
RA Roos D., Caler E., Lorenzi H.;
RL Submitted (APR-2013) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000305}
RP FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR
RP LOCATION, AND DISRUPTION PHENOTYPE.
RC STRAIN=RH {ECO:0000269|PubMed:28205520};
RX PubMed=28205520; DOI=10.1038/ncomms14455;
RA Rajendran E., Hapuarachchi S.V., Miller C.M., Fairweather S.J., Cai Y.,
RA Smith N.C., Cockburn I.A., Broeer S., Kirk K., van Dooren G.G.;
RT "Cationic amino acid transporters play key roles in the survival and
RT transmission of apicomplexan parasites.";
RL Nat. Commun. 8:14455-14455(2017).
RN [3] {ECO:0000305}
RP FUNCTION, NOMENCLATURE, AND MUTAGENESIS OF 54-GLY--ALA-534.
RX PubMed=30742695; DOI=10.1371/journal.ppat.1007577;
RA Parker K.E.R., Fairweather S.J., Rajendran E., Blume M., McConville M.J.,
RA Broeer S., Kirk K., van Dooren G.G.;
RT "The tyrosine transporter of Toxoplasma gondii is a member of the newly
RT defined apicomplexan amino acid transporter (ApiAT) family.";
RL PLoS Pathog. 15:e1007577-e1007577(2019).
CC -!- FUNCTION: Selective L-arginine transporter that is essential for
CC parasite survival and virulence (PubMed:28205520, PubMed:30742695).
CC Does not require other inorganic ions such as sodium, chloride,
CC potassium or calcium (PubMed:28205520). {ECO:0000269|PubMed:28205520,
CC ECO:0000269|PubMed:30742695}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-arginine(in) = L-arginine(out); Xref=Rhea:RHEA:32143,
CC ChEBI:CHEBI:32682; Evidence={ECO:0000269|PubMed:28205520};
CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:32145;
CC Evidence={ECO:0000269|PubMed:28205520};
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=88 uM for arginine {ECO:0000269|PubMed:28205520};
CC pH dependence:
CC Optimum pH is 5-8. {ECO:0000269|PubMed:28205520};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:28205520};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- DISRUPTION PHENOTYPE: Impaired parasite growth under conditions of
CC restricted arginine availability (PubMed:28205520). Does not cause
CC toxoplasmosis in mice (PubMed:28205520). {ECO:0000269|PubMed:28205520}.
CC -!- SIMILARITY: Belongs to the SLC43A transporter (TC 2.A.1.44) family.
CC {ECO:0000305}.
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DR EMBL; KE138836; EPT26443.1; -; Genomic_DNA.
DR RefSeq; XP_002370868.1; XM_002370827.2.
DR EnsemblProtists; TGME49_215490-t26_1; TGME49_215490-t26_1; TGME49_215490.
DR GeneID; 7897047; -.
DR KEGG; tgo:TGME49_215490; -.
DR VEuPathDB; ToxoDB:TGME49_215490; -.
DR HOGENOM; CLU_021456_0_0_1; -.
DR PhylomeDB; S8EZA7; -.
DR Proteomes; UP000001529; Chromosome X.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
DR GO; GO:0061459; F:L-arginine transmembrane transporter activity; IMP:UniProtKB.
DR GO; GO:0097638; P:L-arginine import across plasma membrane; IMP:UniProtKB.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR036259; MFS_trans_sf.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
PE 1: Evidence at protein level;
KW Amino-acid transport; Cell membrane; Glycoprotein; Membrane; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..534
FT /note="Arginine transporter 1"
FT /id="PRO_0000454212"
FT TRANSMEM 35..55
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 99..119
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 126..146
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 154..174
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 182..202
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 216..236
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 329..349
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 365..385
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 388..408
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 419..439
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 451..471
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 483..503
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 261..302
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 246
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT MUTAGEN 54..534
FT /note="Missing: Impaired parasite growth under conditions
FT of restricted arginine availability."
FT /evidence="ECO:0000269|PubMed:30742695"
SQ SEQUENCE 534 AA; 58123 MW; D9FF572736398B51 CRC64;
MAGLLSSCCG RVYALLPDSA HPSAQQKTPF GVNRYVLLVI YMIYALLTSS VYFGWRSMSA
MLFKSGQFSW VCTGESADTS PEEGETDYLC ALQDTKVQSL FTIAMACHFT CSAVAGYLLD
TVGPKAVALL GQTFNALAWI LLAFSGPNFR SVYPAFVFMG AGADVSVYPT LLIVNLFPGS
TALIMATLGA CISLSFFVPL VLRTMWESTG ISFEAVCIGY AVAGPILCAV VAFFFIPFKA
FKGVDNFSAC VEAEKLANSP TAQSSPKAVD SPPCDEGASS RGRLAVSHNT ERTAPDDEQE
KDNTERISLS DLACDPRFKK QKVSFSSQAF TFLYFGICLY FTVCGWVMAY YQEAAGRFLC
NDAEYTLEIL TPLSTIPCLL FGVVINRIGI MPVILMLNTI GLLTYVCVVA AESVVAQYFS
VIFFFMYISI FTTQMYVFVE STFDSAHFGK LIGVASLIGG LLSLISNVLY GDVTVGMLNG
DTRPVVIALL AVIILMYPIL LAMRTKRNRK KQMEQEDIRS RVLELKAAHA ADAA
