PNRC1_HUMAN
ID PNRC1_HUMAN Reviewed; 327 AA.
AC Q12796; B2R6Q0; E1P515; Q5T7J6; Q7Z5N0;
DT 06-JUN-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=Proline-rich nuclear receptor coactivator 1;
DE AltName: Full=Proline-rich protein 2;
DE AltName: Full=Protein B4-2;
GN Name=PNRC1; Synonyms=PROL2;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RX PubMed=7578250; DOI=10.1016/0167-4781(95)00159-e;
RA Chen J., Liu L., Pohajdak B.;
RT "Cloning a cDNA from human NK/T cells which codes for a protein with high
RT proline content.";
RL Biochim. Biophys. Acta 1264:19-22(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RA Zhou G., Ke R., Li H., Cao L., Shen C., Zheng G., Yu R., Zhong G.,
RA Huang F., Lin L., Yang S.;
RL Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=14574404; DOI=10.1038/nature02055;
RA Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L.,
RA Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R.,
RA Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D.,
RA Andrews T.D., Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J.,
RA Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H.,
RA Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J.,
RA Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P.,
RA Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V.,
RA Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J.,
RA Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E.,
RA Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J.,
RA French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J.,
RA Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C.,
RA Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A.,
RA Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R.,
RA Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M.,
RA Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K.,
RA Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R.,
RA Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M.,
RA Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A.,
RA Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L.,
RA Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I.,
RA Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y.,
RA Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E.,
RA Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A.,
RA Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W.,
RA Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M.,
RA West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J.,
RA Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M.,
RA Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I.,
RA Rogers J., Beck S.;
RT "The DNA sequence and analysis of human chromosome 6.";
RL Nature 425:805-811(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Skin;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [7]
RP FUNCTION, INTERACTION WITH NUCLEAR RECEPTORS, MUTAGENESIS OF PRO-287 AND
RP PRO-290, NUCLEAR LOCALIZATION SIGNAL, AND SUBCELLULAR LOCATION.
RX PubMed=10894149; DOI=10.1210/mend.14.7.0480;
RA Zhou D., Quach K.M., Yang C., Lee S.Y., Pohajdak B., Chen S.;
RT "PNRC: a proline-rich nuclear receptor coregulatory protein that modulates
RT transcriptional activation of multiple nuclear receptors including orphan
RT receptors SF1 (steroidogenic factor 1) and ERRalpha1 (estrogen related
RT receptor alpha-1).";
RL Mol. Endocrinol. 14:986-998(2000).
RN [8]
RP TISSUE SPECIFICITY, AND INTERACTION WITH GRB2.
RX PubMed=11574675; DOI=10.1093/nar/29.19.3939;
RA Zhou D., Chen S.;
RT "PNRC2 is a 16 kDa coactivator that interacts with nuclear receptors
RT through an SH3-binding motif.";
RL Nucleic Acids Res. 29:3939-3948(2001).
CC -!- FUNCTION: Nuclear receptor coactivator. May play a role in signal
CC transduction. {ECO:0000269|PubMed:10894149}.
CC -!- SUBUNIT: Interacts with many nuclear receptors including AR, ESR1,
CC ESRRA, ESRRG, NR3C1/GR, NR5A1, PGR, TR, RAR and RXR. Interacts with
CC GRB2. {ECO:0000269|PubMed:10894149, ECO:0000269|PubMed:11574675}.
CC -!- INTERACTION:
CC Q12796; Q9H1D9: POLR3F; NbExp=3; IntAct=EBI-2827376, EBI-710067;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q12796-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q12796-2; Sequence=VSP_055501;
CC -!- TISSUE SPECIFICITY: Expressed in liver, lung, fat and NK/T cells.
CC {ECO:0000269|PubMed:11574675}.
CC -!- DOMAIN: The interaction between PNRC1 and nuclear receptors is
CC dependent on the SH3 binding motif.
CC -!- SIMILARITY: Belongs to the PNRC family. PNRC1 subfamily. {ECO:0000305}.
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DR EMBL; U03105; AAA85576.1; -; mRNA.
DR EMBL; AY303779; AAP76184.1; -; mRNA.
DR EMBL; AK312665; BAG35547.1; -; mRNA.
DR EMBL; AL353135; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471051; EAW48563.1; -; Genomic_DNA.
DR EMBL; CH471051; EAW48564.1; -; Genomic_DNA.
DR EMBL; BC018112; AAH18112.1; -; mRNA.
DR CCDS; CCDS5018.1; -. [Q12796-1]
DR PIR; S59960; S59960.
DR RefSeq; NP_006804.1; NM_006813.2. [Q12796-1]
DR AlphaFoldDB; Q12796; -.
DR SMR; Q12796; -.
DR BioGRID; 116157; 19.
DR IntAct; Q12796; 11.
DR MINT; Q12796; -.
DR STRING; 9606.ENSP00000336931; -.
DR iPTMnet; Q12796; -.
DR PhosphoSitePlus; Q12796; -.
DR BioMuta; PNRC1; -.
DR DMDM; 21362754; -.
DR EPD; Q12796; -.
DR jPOST; Q12796; -.
DR MassIVE; Q12796; -.
DR PaxDb; Q12796; -.
DR PeptideAtlas; Q12796; -.
DR PRIDE; Q12796; -.
DR ProteomicsDB; 58943; -. [Q12796-1]
DR Antibodypedia; 31849; 45 antibodies from 19 providers.
DR DNASU; 10957; -.
DR Ensembl; ENST00000336032.4; ENSP00000336931.3; ENSG00000146278.11. [Q12796-1]
DR GeneID; 10957; -.
DR KEGG; hsa:10957; -.
DR MANE-Select; ENST00000336032.4; ENSP00000336931.3; NM_006813.3; NP_006804.1.
DR UCSC; uc003pmv.4; human. [Q12796-1]
DR CTD; 10957; -.
DR DisGeNET; 10957; -.
DR GeneCards; PNRC1; -.
DR HGNC; HGNC:17278; PNRC1.
DR HPA; ENSG00000146278; Low tissue specificity.
DR MIM; 606714; gene.
DR neXtProt; NX_Q12796; -.
DR OpenTargets; ENSG00000146278; -.
DR PharmGKB; PA33804; -.
DR VEuPathDB; HostDB:ENSG00000146278; -.
DR eggNOG; ENOG502RYBG; Eukaryota.
DR GeneTree; ENSGT00530000063881; -.
DR HOGENOM; CLU_086541_0_0_1; -.
DR InParanoid; Q12796; -.
DR OMA; PSHWMGT; -.
DR OrthoDB; 1030659at2759; -.
DR PhylomeDB; Q12796; -.
DR TreeFam; TF333211; -.
DR PathwayCommons; Q12796; -.
DR SignaLink; Q12796; -.
DR BioGRID-ORCS; 10957; 16 hits in 1088 CRISPR screens.
DR ChiTaRS; PNRC1; human.
DR GeneWiki; PNRC1; -.
DR GenomeRNAi; 10957; -.
DR Pharos; Q12796; Tbio.
DR PRO; PR:Q12796; -.
DR Proteomes; UP000005640; Chromosome 6.
DR RNAct; Q12796; protein.
DR Bgee; ENSG00000146278; Expressed in calcaneal tendon and 201 other tissues.
DR ExpressionAtlas; Q12796; baseline and differential.
DR Genevisible; Q12796; HS.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR InterPro; IPR026780; PNRC1/2.
DR PANTHER; PTHR15405; PTHR15405; 1.
PE 1: Evidence at protein level;
KW Activator; Alternative splicing; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..327
FT /note="Proline-rich nuclear receptor coactivator 1"
FT /id="PRO_0000058482"
FT REGION 66..175
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 280..300
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 94..101
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000269|PubMed:10894149"
FT MOTIF 285..291
FT /note="SH3-binding"
FT COMPBIAS 111..125
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 102..180
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.2"
FT /id="VSP_055501"
FT VARIANT 252
FT /note="P -> L (in dbSNP:rs2231277)"
FT /id="VAR_051284"
FT MUTAGEN 287
FT /note="P->A: Abolishes the interaction with the nuclear
FT receptors; when associated with A-290."
FT /evidence="ECO:0000269|PubMed:10894149"
FT MUTAGEN 290
FT /note="P->A: Abolishes the interaction with the nuclear
FT receptors; when associated with A-287."
FT /evidence="ECO:0000269|PubMed:10894149"
FT CONFLICT 218
FT /note="Y -> N (in Ref. 2; AAP76184)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 327 AA; 35225 MW; 97E0D65C2E690763 CRC64;
MTVVSVPQRE PLVLGGRLAP LGFSSRGYFG ALPMVTTAPP PLPRIPDPRA LPPTLFLPHF
LGGDGPCLTP QPRAPAALPN RSLAVAGGTP RAAPKKRRKK KVRASPAGQL PSRFHQYQQH
RPSLEGGRSP ATGPSGAQEV PGPAAALAPS PAAAAGTEGA SPDLAPLRPA APGQTPLRKE
VLKSKMGKSE KIALPHGQLV HGIHLYEQPK INRQKSKYNL PLTKITSAKR NENNFWQDSV
SSDRIQKQEK KPFKNTENIK NSHLKKSAFL TEVSQKENYA GAKFSDPPSP SVLPKPPSHW
MGSTVENSNQ NRELMAVHLK TLLKVQT
