ID   PNS1_CRYNJ              Reviewed;         551 AA.
AC   P0CM92; Q561D1; Q5KQ32;
DT   28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT   28-JUN-2011, sequence version 1.
DT   25-MAY-2022, entry version 33.
DE   RecName: Full=Protein PNS1;
GN   Name=PNS1; OrderedLocusNames=CNA00640;
OS   Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC
OS   MYA-565) (Filobasidiella neoformans).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes;
OC   Tremellales; Cryptococcaceae; Cryptococcus;
OC   Cryptococcus neoformans species complex.
OX   NCBI_TaxID=214684;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JEC21 / ATCC MYA-565;
RX   PubMed=15653466; DOI=10.1126/science.1103773;
RA   Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D.,
RA   Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E.,
RA   Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A.,
RA   Fox D.S., Grinberg V., Fu J., Fukushima M., Haas B.J., Huang J.C.,
RA   Janbon G., Jones S.J.M., Koo H.L., Krzywinski M.I., Kwon-Chung K.J.,
RA   Lengeler K.B., Maiti R., Marra M.A., Marra R.E., Mathewson C.A.,
RA   Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L., Schein J.E.,
RA   Shvartsbeyn A., Shin H., Shumway M., Specht C.A., Suh B.B., Tenney A.,
RA   Utterback T.R., Wickes B.L., Wortman J.R., Wye N.H., Kronstad J.W.,
RA   Lodge J.K., Heitman J., Davis R.W., Fraser C.M., Hyman R.W.;
RT   "The genome of the basidiomycetous yeast and human pathogen Cryptococcus
RT   neoformans.";
RL   Science 307:1321-1324(2005).
CC   -!- FUNCTION: Probably involved in transport through the plasma membrane.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC       protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the CTL (choline transporter-like) family.
CC       {ECO:0000305}.
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DR   EMBL; AE017341; AAW41263.1; -; Genomic_DNA.
DR   RefSeq; XP_567082.1; XM_567082.1.
DR   AlphaFoldDB; P0CM92; -.
DR   STRING; 5207.AAW41263; -.
DR   PaxDb; P0CM92; -.
DR   EnsemblFungi; AAW41263; AAW41263; CNA00640.
DR   GeneID; 3253742; -.
DR   KEGG; cne:CNA00640; -.
DR   VEuPathDB; FungiDB:CNA00640; -.
DR   eggNOG; KOG1362; Eukaryota.
DR   HOGENOM; CLU_026724_0_0_1; -.
DR   InParanoid; P0CM92; -.
DR   OMA; FTKQFIW; -.
DR   OrthoDB; 1276753at2759; -.
DR   Proteomes; UP000002149; Chromosome 1.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0016020; C:membrane; IBA:GO_Central.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR   GO; GO:0055085; P:transmembrane transport; IBA:GO_Central.
DR   InterPro; IPR007603; Choline_transptr-like.
DR   PANTHER; PTHR12385; PTHR12385; 1.
DR   Pfam; PF04515; Choline_transpo; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..551
FT                   /note="Protein PNS1"
FT                   /id="PRO_0000191733"
FT   TOPO_DOM        1..94
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        95..115
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        116..147
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        148..168
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        169..177
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        178..198
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        199..200
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        201..221
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        222..244
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        245..265
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        266..307
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        308..328
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        329..356
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        357..377
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        378..386
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        387..407
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        408..452
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        453..473
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        474..488
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        489..509
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        510..551
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   REGION          1..72
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..19
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        49..72
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   551 AA;  60043 MW;  34160E1E434F968F CRC64;
     MSAQEFYQGG NQRGYQQQQF PPPPGGPPQD QNGGKQEYVP PQGQPPNYNM KPSQPYASTN
     PETGGQPVYQ DTAPFSQANE KTGERMNPRK RVNDIIPLIL FIAAVVGFAV VSGIAIHGFV
     QVNGLGGGMG DSSIGRTGSS ITLDYHTVYL LLVVVALGLV IASLYLAALR AFTKIILEVT
     LALTVILNIG ICIYYFIIQY WSGAIIFLII ALVSVFFYWG MRKRIPLAKL LLQTTIDVTK
     HHPSVYVVVF IGLIIQAAVS VWYTFTCIAI YVKWTPGSAA CSDGGCSSSK VAGLVFYATF
     SYLWLSQVIG NVILCTLAGG VFGGWYYYGP RTPGGGVPKR ASLLAFVRAS TLSLGSIAFG
     SLLVTILELL RLILQLFRQY EAGQGDMIGS ILICIAQCCI GCIQWMVEYF NKYAYIEIAL
     YGKSYIPAAK DTWRLLKDRG IDALVNDSLV GTALMWGAYI NGFLCAVLGY FYLRFTHPAY
     NSDGQYSAPV ILFSFLIGLN ESFTVGSAID AGVSTIFVGL GEDPMVLAER SPGLFEMIRQ
     VYPRVVQGVP H