PNSB1_ARATH
ID PNSB1_ARATH Reviewed; 461 AA.
AC Q9S9N6;
DT 04-FEB-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=Photosynthetic NDH subunit of subcomplex B 1, chloroplastic {ECO:0000303|PubMed:21785130};
DE Short=Protein PnsB1 {ECO:0000303|PubMed:21785130};
DE AltName: Full=NAD(P)H DEHYDROGENASE SUBUNIT 48 {ECO:0000303|PubMed:18974055};
DE AltName: Full=NDH-DEPENDENT CYCLIC ELECTRON FLOW 1 {ECO:0000303|PubMed:18785996};
DE Flags: Precursor;
GN Name=PNSB1 {ECO:0000303|PubMed:21785130};
GN Synonyms=GT9 {ECO:0000303|PubMed:24905498},
GN NDF1 {ECO:0000303|PubMed:18785996}, NDH48 {ECO:0000303|PubMed:18974055};
GN OrderedLocusNames=At1g15980 {ECO:0000312|Araport:AT1G15980};
GN ORFNames=T24D18.8 {ECO:0000312|EMBL:AAF18495.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=24905498; DOI=10.1111/tpj.12577;
RA Lao J., Oikawa A., Bromley J.R., McInerney P., Suttangkakul A.,
RA Smith-Moritz A.M., Plahar H., Chiu T.-Y., Gonzalez Fernandez-Nino S.M.G.,
RA Ebert B., Yang F., Christiansen K.M., Hansen S.F., Stonebloom S.,
RA Adams P.D., Ronald P.C., Hillson N.J., Hadi M.Z., Vega-Sanchez M.E.,
RA Loque D., Scheller H.V., Heazlewood J.L.;
RT "The plant glycosyltransferase clone collection for functional genomics.";
RL Plant J. 79:517-529(2014).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP COMPONENT OF THE NDH COMPLEX, SUBCELLULAR LOCATION, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=18974055; DOI=10.1074/jbc.m805404200;
RA Sirpio S., Allahverdiyeva Y., Holmstrom M., Khrouchtchova A., Haldrup A.,
RA Battchikova N., Aro E.M.;
RT "Novel nuclear-encoded subunits of the chloroplast NAD(P)H dehydrogenase
RT complex.";
RL J. Biol. Chem. 284:905-912(2009).
RN [7]
RP REVIEW.
RX PubMed=19995722; DOI=10.1093/mp/ssp052;
RA Suorsa M., Sirpioe S., Aro E.M.;
RT "Towards characterization of the chloroplast NAD(P)H dehydrogenase
RT complex.";
RL Mol. Plant 2:1127-1140(2009).
RN [8]
RP COMPONENT OF THE NDH COMPLEX, SUBCELLULAR LOCATION, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=18785996; DOI=10.1111/j.1365-313x.2008.03680.x;
RA Takabayashi A., Ishikawa N., Obayashi T., Ishida S., Obokata J., Endo T.,
RA Sato F.;
RT "Three novel subunits of Arabidopsis chloroplastic NAD(P)H dehydrogenase
RT identified by bioinformatic and reverse genetic approaches.";
RL Plant J. 57:207-219(2009).
RN [9]
RP REVIEW.
RX PubMed=21029720; DOI=10.1016/j.bbabio.2010.10.015;
RA Peng L., Yamamoto H., Shikanai T.;
RT "Structure and biogenesis of the chloroplast NAD(P)H dehydrogenase
RT complex.";
RL Biochim. Biophys. Acta 1807:945-953(2011).
RN [10]
RP NOMENCLATURE, AND COMPONENT OF THE NDH COMPLEX.
RX PubMed=21785130; DOI=10.1093/pcp/pcr098;
RA Ifuku K., Endo T., Shikanai T., Aro E.M.;
RT "Structure of the chloroplast NADH dehydrogenase-like complex: nomenclature
RT for nuclear-encoded subunits.";
RL Plant Cell Physiol. 52:1560-1568(2011).
CC -!- FUNCTION: NDH shuttles electrons from NAD(P)H:plastoquinone, via FMN
CC and iron-sulfur (Fe-S) centers, to quinones in the photosynthetic chain
CC and possibly in a chloroplast respiratory chain. The immediate electron
CC acceptor for the enzyme in this species is believed to be
CC plastoquinone. Couples the redox reaction to proton translocation, and
CC thus conserves the redox energy in a proton gradient. {ECO:0000305}.
CC -!- SUBUNIT: Part of the chloroplast NDH complex, composed of a mixture of
CC chloroplast and nucleus encoded subunits. Component of the NDH
CC subcomplex B, at least composed of PnsB1, PnsB2, PnsB3, PnsB4 and
CC PnsB5. {ECO:0000269|PubMed:18785996, ECO:0000269|PubMed:18974055,
CC ECO:0000269|PubMed:21785130}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC {ECO:0000269|PubMed:18785996, ECO:0000269|PubMed:18974055}; Peripheral
CC membrane protein {ECO:0000269|PubMed:18974055}; Stromal side
CC {ECO:0000269|PubMed:18974055}.
CC -!- DISRUPTION PHENOTYPE: Malfunction of the NDH complex.
CC {ECO:0000269|PubMed:18785996, ECO:0000269|PubMed:18974055}.
CC -!- CAUTION: Was erroneously thought to be a glycosyltransferase.
CC {ECO:0000303|PubMed:24905498}.
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DR EMBL; KJ139005; AHL38945.1; -; mRNA.
DR EMBL; AC010924; AAF18495.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE29392.1; -; Genomic_DNA.
DR EMBL; AY057703; AAL15333.1; -; mRNA.
DR EMBL; AY124875; AAM70584.1; -; mRNA.
DR EMBL; AY084966; AAM61527.1; -; mRNA.
DR PIR; D86294; D86294.
DR RefSeq; NP_563986.1; NM_101466.3.
DR PDB; 7WFF; EM; 3.59 A; a=1-461.
DR PDB; 7WG5; EM; 3.89 A; a=1-461.
DR PDBsum; 7WFF; -.
DR PDBsum; 7WG5; -.
DR AlphaFoldDB; Q9S9N6; -.
DR SMR; Q9S9N6; -.
DR STRING; 3702.AT1G15980.1; -.
DR TCDB; 3.D.1.8.1; the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.
DR PaxDb; Q9S9N6; -.
DR PRIDE; Q9S9N6; -.
DR ProteomicsDB; 234692; -.
DR EnsemblPlants; AT1G15980.1; AT1G15980.1; AT1G15980.
DR GeneID; 838168; -.
DR Gramene; AT1G15980.1; AT1G15980.1; AT1G15980.
DR KEGG; ath:AT1G15980; -.
DR Araport; AT1G15980; -.
DR TAIR; locus:2200452; AT1G15980.
DR eggNOG; ENOG502QQIV; Eukaryota.
DR HOGENOM; CLU_585830_0_0_1; -.
DR InParanoid; Q9S9N6; -.
DR OMA; PLEHWAE; -.
DR OrthoDB; 628504at2759; -.
DR PhylomeDB; Q9S9N6; -.
DR PRO; PR:Q9S9N6; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9S9N6; baseline and differential.
DR Genevisible; Q9S9N6; AT.
DR GO; GO:0009507; C:chloroplast; HDA:TAIR.
DR GO; GO:0009534; C:chloroplast thylakoid; HDA:TAIR.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; IDA:UniProtKB.
DR GO; GO:0010598; C:NAD(P)H dehydrogenase complex (plastoquinone); IDA:TAIR.
DR GO; GO:0005634; C:nucleus; HDA:TAIR.
DR GO; GO:0009773; P:photosynthetic electron transport in photosystem I; IMP:TAIR.
DR InterPro; IPR044983; PNSB1.
DR PANTHER; PTHR37698; PTHR37698; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Chloroplast; Membrane; Plastid; Reference proteome;
KW Thylakoid; Transit peptide; Transport.
FT TRANSIT 1..44
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 45..461
FT /note="Photosynthetic NDH subunit of subcomplex B 1,
FT chloroplastic"
FT /id="PRO_0000431819"
FT REGION 66..90
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 461 AA; 51022 MW; 886C7007C64C88E3 CRC64;
MASSLPLLPK PISPFFKTPP FSTSKPLVFL NFQTRLTSRS SDVSVNLKKK NNPWLDPFDS
GEDPDNEYGS LFADGKQDED PRPPDNPDNP YGFLKFPKGY TVELASLPLK IRGDVRRCCC
VISGGVYENL LFFPTIQLIK DRYPGVQVDI LTTERGKQTY ELNKNVRWAN VYDPDDHWPE
PAEYTDMIGL LKGRYYDMVL STKLAGLGHA AFLFMTTARD RVSYIYPNVN SAGAGLMLSE
TFTAENTNLS ELGYSMYTQM EDWLGRPFRS VPRTPLLPLR VSISRKVKEV VAAKYRNAGA
VTGKFIVIHG IESDSKASMQ SKGDADSLLS LEKWAKIIKG VRGFKPVFVI PHEKERENVE
DFVGDDTSIV FITTPGQLAA LINDSAGVIA TNTAAIQLAN ARDKPCIGLF SSEEKGKLFV
PYAEEKSNCV IIASKTGKLA DIDIGTVKNA MQVFEGSLAL V
