ID   PNSB4_ARATH             Reviewed;         175 AA.
AC   Q8RXS1; Q9M9U4;
DT   04-FEB-2015, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   03-AUG-2022, entry version 110.
DE   RecName: Full=Photosynthetic NDH subunit of subcomplex B 4, chloroplastic {ECO:0000303|PubMed:21785130};
DE            Short=Protein PnsB4 {ECO:0000303|PubMed:21785130};
DE   AltName: Full=NDH DEPENDENT FLOW 6 {ECO:0000303|PubMed:18535009};
DE   AltName: Full=NDH-DEPENDENT CYCLIC ELECTRON FLOW 6 {ECO:0000305};
DE   Flags: Precursor;
GN   Name=PNSB4 {ECO:0000303|PubMed:21785130};
GN   Synonyms=NDF6 {ECO:0000303|PubMed:18535009};
GN   OrderedLocusNames=At1g18730 {ECO:0000312|Araport:AT1G18730};
GN   ORFNames=F6A14.16 {ECO:0000312|EMBL:AAF27106.1};
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130712; DOI=10.1038/35048500;
RA   Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA   Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA   Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA   Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA   Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA   Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA   Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA   Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA   Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA   Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA   Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA   Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA   Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA   Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA   Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT   "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL   Nature 408:816-820(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA   Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA   Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA   Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA   Shinozaki K.;
RT   "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL   Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   COMPONENT OF THE NDH COMPLEX, SUBCELLULAR LOCATION, AND DISRUPTION
RP   PHENOTYPE.
RX   PubMed=18535009; DOI=10.1093/pcp/pcn083;
RA   Ishikawa N., Takabayashi A., Ishida S., Hano Y., Endo T., Sato F.;
RT   "NDF6: a thylakoid protein specific to terrestrial plants is essential for
RT   activity of chloroplastic NAD(P)H dehydrogenase in Arabidopsis.";
RL   Plant Cell Physiol. 49:1066-1073(2008).
RN   [6]
RP   REVIEW.
RX   PubMed=19995722; DOI=10.1093/mp/ssp052;
RA   Suorsa M., Sirpioe S., Aro E.M.;
RT   "Towards characterization of the chloroplast NAD(P)H dehydrogenase
RT   complex.";
RL   Mol. Plant 2:1127-1140(2009).
RN   [7]
RP   REVIEW.
RX   PubMed=21029720; DOI=10.1016/j.bbabio.2010.10.015;
RA   Peng L., Yamamoto H., Shikanai T.;
RT   "Structure and biogenesis of the chloroplast NAD(P)H dehydrogenase
RT   complex.";
RL   Biochim. Biophys. Acta 1807:945-953(2011).
RN   [8]
RP   NOMENCLATURE, AND COMPONENT OF THE NDH COMPLEX.
RX   PubMed=21785130; DOI=10.1093/pcp/pcr098;
RA   Ifuku K., Endo T., Shikanai T., Aro E.M.;
RT   "Structure of the chloroplast NADH dehydrogenase-like complex: nomenclature
RT   for nuclear-encoded subunits.";
RL   Plant Cell Physiol. 52:1560-1568(2011).
CC   -!- FUNCTION: NDH shuttles electrons from NAD(P)H:plastoquinone, via FMN
CC       and iron-sulfur (Fe-S) centers, to quinones in the photosynthetic chain
CC       and possibly in a chloroplast respiratory chain. The immediate electron
CC       acceptor for the enzyme in this species is believed to be
CC       plastoquinone. Couples the redox reaction to proton translocation, and
CC       thus conserves the redox energy in a proton gradient. {ECO:0000305}.
CC   -!- SUBUNIT: Part of the chloroplast NDH complex, composed of a mixture of
CC       chloroplast and nucleus encoded subunits. Component of the NDH
CC       subcomplex B, at least composed of PnsB1, PnsB2, PnsB3, PnsB4 and
CC       PnsB5. {ECO:0000269|PubMed:18535009, ECO:0000269|PubMed:21785130}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC       {ECO:0000269|PubMed:18535009}; Single-pass membrane protein
CC       {ECO:0000255}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=1;
CC         Comment=A number of isoforms are produced. According to EST
CC         sequences.;
CC       Name=1;
CC         IsoId=Q8RXS1-1; Sequence=Displayed;
CC   -!- DISRUPTION PHENOTYPE: Malfunction of the NDH complex.
CC       {ECO:0000269|PubMed:18535009}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAF27106.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AC011809; AAF27106.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002684; AEE29753.1; -; Genomic_DNA.
DR   EMBL; AY117165; AAM51240.1; -; mRNA.
DR   EMBL; AK226496; BAE98638.1; -; mRNA.
DR   EMBL; AY080701; AAL85020.1; -; mRNA.
DR   PIR; B86321; B86321.
DR   RefSeq; NP_173308.2; NM_101731.5. [Q8RXS1-1]
DR   AlphaFoldDB; Q8RXS1; -.
DR   SMR; Q8RXS1; -.
DR   IntAct; Q8RXS1; 15.
DR   STRING; 3702.AT1G18730.1; -.
DR   PaxDb; Q8RXS1; -.
DR   PRIDE; Q8RXS1; -.
DR   EnsemblPlants; AT1G18730.1; AT1G18730.1; AT1G18730. [Q8RXS1-1]
DR   GeneID; 838455; -.
DR   Gramene; AT1G18730.1; AT1G18730.1; AT1G18730. [Q8RXS1-1]
DR   KEGG; ath:AT1G18730; -.
DR   Araport; AT1G18730; -.
DR   TAIR; locus:2034980; AT1G18730.
DR   eggNOG; ENOG502RXY2; Eukaryota.
DR   InParanoid; Q8RXS1; -.
DR   OrthoDB; 1435497at2759; -.
DR   PhylomeDB; Q8RXS1; -.
DR   PRO; PR:Q8RXS1; -.
DR   Proteomes; UP000006548; Chromosome 1.
DR   ExpressionAtlas; Q8RXS1; baseline and differential.
DR   Genevisible; Q8RXS1; AT.
DR   GO; GO:0009535; C:chloroplast thylakoid membrane; IDA:UniProtKB.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0010598; C:NAD(P)H dehydrogenase complex (plastoquinone); IMP:UniProtKB.
DR   GO; GO:0009773; P:photosynthetic electron transport in photosystem I; TAS:TAIR.
DR   InterPro; IPR034570; PNSB4.
DR   PANTHER; PTHR36315; PTHR36315; 1.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Chloroplast; Membrane; Plastid; Reference proteome;
KW   Thylakoid; Transit peptide; Transmembrane; Transmembrane helix; Transport.
FT   TRANSIT         1..24
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000255"
FT   CHAIN           25..175
FT                   /note="Photosynthetic NDH subunit of subcomplex B 4,
FT                   chloroplastic"
FT                   /id="PRO_0000431822"
FT   TRANSMEM        95..111
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   175 AA;  20396 MW;  8D2215B297A1383E CRC64;
     MAEAFTSFTF TNLHIPSSYN HSPKQNSGPN HGYWLSKNVN EKRERNLMRG SLCVRKALPH
     DLPLMAVMVQ QIEGMRDIIT EKHVWHLSDK AIKNVYMFYI MFTCWGCLYF GSAKDPFYDS
     EEYRGDGGDG TGYWVYETQE DIEEKARAEL WREELIEEIE QKVGGLRELE EAVTK