PNSB5_ARATH
ID PNSB5_ARATH Reviewed; 212 AA.
AC Q9FG89; Q8LE15;
DT 04-FEB-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=Photosynthetic NDH subunit of subcomplex B 5, chloroplastic {ECO:0000303|PubMed:21785130};
DE Short=Protein PnsB5 {ECO:0000303|PubMed:21785130};
DE AltName: Full=NAD(P)H dehydrogenase 18 {ECO:0000312|EMBL:AED95005.1};
DE Flags: Precursor;
GN Name=PNSB5 {ECO:0000303|PubMed:21785130};
GN Synonyms=NDH18 {ECO:0000303|PubMed:19903870};
GN OrderedLocusNames=At5g43750 {ECO:0000312|Araport:AT5G43750};
GN ORFNames=MQD19.9 {ECO:0000312|EMBL:BAB11302.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RA Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. XI.";
RL Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP REVIEW.
RX PubMed=19995722; DOI=10.1093/mp/ssp052;
RA Suorsa M., Sirpioe S., Aro E.M.;
RT "Towards characterization of the chloroplast NAD(P)H dehydrogenase
RT complex.";
RL Mol. Plant 2:1127-1140(2009).
RN [6]
RP COMPONENT OF THE NDH COMPLEX.
RX PubMed=19903870; DOI=10.1105/tpc.109.068791;
RA Peng L., Fukao Y., Fujiwara M., Takami T., Shikanai T.;
RT "Efficient operation of NAD(P)H dehydrogenase requires supercomplex
RT formation with photosystem I via minor LHCI in Arabidopsis.";
RL Plant Cell 21:3623-3640(2009).
RN [7]
RP REVIEW.
RX PubMed=21029720; DOI=10.1016/j.bbabio.2010.10.015;
RA Peng L., Yamamoto H., Shikanai T.;
RT "Structure and biogenesis of the chloroplast NAD(P)H dehydrogenase
RT complex.";
RL Biochim. Biophys. Acta 1807:945-953(2011).
RN [8]
RP NOMENCLATURE, AND COMPONENT OF THE NDH COMPLEX.
RX PubMed=21785130; DOI=10.1093/pcp/pcr098;
RA Ifuku K., Endo T., Shikanai T., Aro E.M.;
RT "Structure of the chloroplast NADH dehydrogenase-like complex: nomenclature
RT for nuclear-encoded subunits.";
RL Plant Cell Physiol. 52:1560-1568(2011).
CC -!- FUNCTION: NDH shuttles electrons from NAD(P)H:plastoquinone, via FMN
CC and iron-sulfur (Fe-S) centers, to quinones in the photosynthetic chain
CC and possibly in a chloroplast respiratory chain. The immediate electron
CC acceptor for the enzyme in this species is believed to be
CC plastoquinone. Couples the redox reaction to proton translocation, and
CC thus conserves the redox energy in a proton gradient. {ECO:0000305}.
CC -!- SUBUNIT: Part of the chloroplast NDH complex, composed of a mixture of
CC chloroplast and nucleus encoded subunits. Component of the NDH
CC subcomplex B, at least composed of PnsB1, PnsB2, PnsB3, PnsB4 and
CC PnsB5. {ECO:0000269|PubMed:19903870, ECO:0000269|PubMed:21785130}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane {ECO:0000255};
CC Multi-pass membrane protein {ECO:0000255}.
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DR EMBL; AB026651; BAB11302.1; -; Genomic_DNA.
DR EMBL; CP002688; AED95005.1; -; Genomic_DNA.
DR EMBL; BT004156; AAO42177.1; -; mRNA.
DR EMBL; BT005490; AAO63910.1; -; mRNA.
DR EMBL; AY085676; AAM62895.1; -; mRNA.
DR RefSeq; NP_568629.1; NM_123742.3.
DR PDB; 7WFF; EM; 3.59 A; e=1-212.
DR PDB; 7WG5; EM; 3.89 A; e=1-212.
DR PDBsum; 7WFF; -.
DR PDBsum; 7WG5; -.
DR AlphaFoldDB; Q9FG89; -.
DR SMR; Q9FG89; -.
DR IntAct; Q9FG89; 12.
DR STRING; 3702.AT5G43750.1; -.
DR TCDB; 3.D.1.8.1; the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.
DR PaxDb; Q9FG89; -.
DR PRIDE; Q9FG89; -.
DR ProteomicsDB; 234695; -.
DR EnsemblPlants; AT5G43750.1; AT5G43750.1; AT5G43750.
DR GeneID; 834396; -.
DR Gramene; AT5G43750.1; AT5G43750.1; AT5G43750.
DR KEGG; ath:AT5G43750; -.
DR Araport; AT5G43750; -.
DR TAIR; locus:2170827; AT5G43750.
DR eggNOG; ENOG502R1NE; Eukaryota.
DR HOGENOM; CLU_077245_1_0_1; -.
DR InParanoid; Q9FG89; -.
DR OMA; QIYKLER; -.
DR OrthoDB; 1151977at2759; -.
DR PhylomeDB; Q9FG89; -.
DR PRO; PR:Q9FG89; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FG89; baseline and differential.
DR Genevisible; Q9FG89; AT.
DR GO; GO:0009507; C:chloroplast; HDA:TAIR.
DR GO; GO:0031969; C:chloroplast membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; HDA:TAIR.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009536; C:plastid; HDA:TAIR.
DR GO; GO:0006979; P:response to oxidative stress; IMP:TAIR.
DR InterPro; IPR034569; PNSB5.
DR PANTHER; PTHR36399; PTHR36399; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Chloroplast; Membrane; Plastid; Reference proteome;
KW Transit peptide; Transmembrane; Transmembrane helix; Transport.
FT TRANSIT 1..48
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 49..212
FT /note="Photosynthetic NDH subunit of subcomplex B 5,
FT chloroplastic"
FT /id="PRO_0000431823"
FT TRANSMEM 115..135
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 136..156
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT CONFLICT 28
FT /note="I -> T (in Ref. 4; AAM62895)"
FT /evidence="ECO:0000305"
FT CONFLICT 35..36
FT /note="GK -> SN (in Ref. 4; AAM62895)"
FT /evidence="ECO:0000305"
FT CONFLICT 42
FT /note="K -> R (in Ref. 4; AAM62895)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 212 AA; 23746 MW; 19031FB50CF3CCCF CRC64;
MATVTILSPK SIPKVTDSKF GARVSDQIVN VVKCGKSGRR LKLAKLVSAA GLSQIEPDIN
EDPIGQFETN SIEMEDFKYG YYDGAHTYYE GEVQKGTFWG AIADDIAAVD QTNGFQGLIS
CMFLPAIALG MYFDAPGEYL FIGAALFTVV FCIIEMDKPD QPHNFEPQIY KLERGARDKL
INDYNTMSIW DFNDKYGDVW DFTIEKDDIA TR
