PNT_DROME
ID PNT_DROME Reviewed; 718 AA.
AC P51023; A0A0B4KH96; B9EQV8; H8F4R1; P19420; P51022; Q29R53; Q8IG92; Q8IMZ1;
AC Q9VCN2;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 16-NOV-2001, sequence version 2.
DT 03-AUG-2022, entry version 201.
DE RecName: Full=ETS-like protein pointed {ECO:0000305};
GN Name=pnt {ECO:0000312|FlyBase:FBgn0003118};
GN Synonyms=DMPOINT1A {ECO:0000312|FlyBase:FBgn0003118},
GN ETS2 {ECO:0000312|FlyBase:FBgn0003118},
GN Ets58AB {ECO:0000303|PubMed:2834248},
GN pointed {ECO:0000312|FlyBase:FBgn0003118}; ORFNames=CG17077;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS P1 AND P2), FUNCTION, DEVELOPMENTAL
RP STAGE, DISRUPTION PHENOTYPE, AND DOMAIN ETS.
RX PubMed=8223245; DOI=10.1242/dev.117.1.163;
RA Klaembt C.;
RT "The Drosophila gene pointed encodes two ETS-like proteins which are
RT involved in the development of the midline glial cells.";
RL Development 117:163-176(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS D AND P2).
RC STRAIN=Berkeley {ECO:0000312|EMBL:ABC86249.1};
RC TISSUE=Embryo {ECO:0000312|EMBL:ACM16754.1};
RA Stapleton M., Booth B., Kapadia B., Carlson J., Chavez C., Frise E.,
RA George R., Pacleb J., Park S., Wan K., Yu C., Celniker S.;
RL Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-323 (ISOFORM P2).
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAN71682.1}; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 540-698, AND DEVELOPMENTAL STAGE.
RX PubMed=2834248; DOI=10.1016/0012-1606(88)90187-x;
RA Pribyl L.J., Watson D.K., McWilliams M.J., Ascione R., Papas T.S.;
RT "The Drosophila ets-2 gene: molecular structure, chromosomal localization,
RT and developmental expression.";
RL Dev. Biol. 127:45-53(1988).
RN [7]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 551-708 (ISOFORM P2), DEVELOPMENTAL STAGE,
RP AND DOMAIN ETS.
RC STRAIN=Canton-S {ECO:0000312|EMBL:AAC34200.1};
RC TISSUE=Larva {ECO:0000312|EMBL:AAC34200.1};
RX PubMed=1577186; DOI=10.1016/0012-1606(92)90225-6;
RA Chen T., Bunting M., Karim F.D., Thummel C.S.;
RT "Isolation and characterization of five Drosophila genes that encode an
RT ets-related DNA binding domain.";
RL Dev. Biol. 151:176-191(1992).
RN [8]
RP FUNCTION, DEVELOPMENTAL STAGE, PHOSPHORYLATION AT THR-151, DISRUPTION
RP PHENOTYPE, AND MUTAGENESIS OF THR-151.
RX PubMed=8033205; DOI=10.1016/0092-8674(94)90580-0;
RA O'Neill E.M., Rebay I., Tjian R., Rubin G.M.;
RT "The activities of two Ets-related transcription factors required for
RT Drosophila eye development are modulated by the Ras/MAPK pathway.";
RL Cell 78:137-147(1994).
RN [9]
RP FUNCTION, SUBCELLULAR LOCATION, AND DEVELOPMENTAL STAGE.
RX PubMed=22143802; DOI=10.1073/pnas.1118595109;
RA Zhu S., Barshow S., Wildonger J., Jan L.Y., Jan Y.N.;
RT "Ets transcription factor Pointed promotes the generation of intermediate
RT neural progenitors in Drosophila larval brains.";
RL Proc. Natl. Acad. Sci. U.S.A. 108:20615-20620(2011).
RN [10]
RP FUNCTION, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=23757412; DOI=10.1242/dev.093138;
RA Shwartz A., Yogev S., Schejter E.D., Shilo B.Z.;
RT "Sequential activation of ETS proteins provides a sustained transcriptional
RT response to EGFR signaling.";
RL Development 140:2746-2754(2013).
RN [11]
RP FUNCTION, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=27151950; DOI=10.1242/dev.136184;
RA Li X., Xie Y., Zhu S.;
RT "Notch maintains Drosophila type II neuroblasts by suppressing expression
RT of the Fez transcription factor Earmuff.";
RL Development 143:2511-2521(2016).
RN [12]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=27510969; DOI=10.1242/dev.137281;
RA Xie Y., Li X., Deng X., Hou Y., O'Hara K., Urso A., Peng Y., Chen L.,
RA Zhu S.;
RT "The Ets protein Pointed prevents both premature differentiation and
RT dedifferentiation of Drosophila intermediate neural progenitors.";
RL Development 143:3109-3118(2016).
RN [13]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=28899667; DOI=10.1016/j.ydbio.2017.09.011;
RA Li X., Chen R., Zhu S.;
RT "bHLH-O proteins balance the self-renewal and differentiation of Drosophila
RT neural stem cells by regulating Earmuff expression.";
RL Dev. Biol. 431:239-251(2017).
RN [14]
RP FUNCTION, DEVELOPMENTAL STAGE, AND DOMAIN ETS.
RX PubMed=28245922; DOI=10.1016/j.devcel.2017.01.014;
RA Janssens D.H., Hamm D.C., Anhezini L., Xiao Q., Siller K.H., Siegrist S.E.,
RA Harrison M.M., Lee C.Y.;
RT "An Hdac1/Rpd3-poised circuit balances continual self-renewal and rapid
RT restriction of developmental potential during asymmetric stem cell
RT division.";
RL Dev. Cell 40:367-380(2017).
CC -!- FUNCTION: ETS transcription factor with a prominent role during
CC development of the eye and the nervous system (PubMed:8223245,
CC PubMed:8033205, PubMed:23757412, PubMed:28245922). Required for glial-
CC neuronal cell interactions at the ventral midline which are necessary
CC for the proper elaboration of commissures in the embryonic CNS
CC (PubMed:8223245). {ECO:0000269|PubMed:23757412,
CC ECO:0000269|PubMed:28245922, ECO:0000269|PubMed:8033205,
CC ECO:0000269|PubMed:8223245}.
CC -!- FUNCTION: [Isoform P2]: Required for normal EGFR-induced photoreceptor
CC development probably as a downstream effector of Ras85D
CC (PubMed:8033205, PubMed:23757412). In larval eye imaginal disks,
CC activated by MAPK phosphorylation following EGFR activation, induces
CC transcription of isoform P1 which in turn activates transcription of
CC target genes essential for photoreceptor development (PubMed:23757412).
CC -!- FUNCTION: [Isoform P1]: Required for normal EGFR-induced photoreceptor
CC development (PubMed:23757412). Following transcriptional activation by
CC isoform P2, acts as a constitutive activator of transcription, leading
CC to induction of target genes essential for photoreceptor development
CC (PubMed:8033205, PubMed:23757412). In larval brains, involved in the
CC maintenance of type II neuroblast self-renewal and identity together
CC with brat, btd and pros; prevents intermediate neuronal progenitor
CC (INP) dedifferentiation by regulating the expression of erm probably
CC via Notch signaling; suppresses Ase expression in type II neuroblasts
CC and promotes the generation of intermediate neuronal progenitors
CC (PubMed:22143802, PubMed:27151950, PubMed:27510969, PubMed:28899667,
CC PubMed:28245922). {ECO:0000269|PubMed:22143802,
CC ECO:0000269|PubMed:23757412, ECO:0000269|PubMed:27151950,
CC ECO:0000269|PubMed:27510969, ECO:0000269|PubMed:28245922,
CC ECO:0000269|PubMed:28899667, ECO:0000269|PubMed:8033205}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:22143802}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=P2 {ECO:0000303|PubMed:8223245}; Synonyms=B
CC {ECO:0000312|FlyBase:FBgn0003118};
CC IsoId=P51023-1; Sequence=Displayed;
CC Name=D {ECO:0000312|FlyBase:FBgn0003118};
CC IsoId=P51023-2; Sequence=VSP_059597;
CC Name=P1 {ECO:0000303|PubMed:8223245}; Synonyms=C
CC {ECO:0000312|FlyBase:FBgn0003118};
CC IsoId=P51023-3, P51022-1;
CC Sequence=VSP_059596;
CC Name=E {ECO:0000312|FlyBase:FBgn0003118};
CC IsoId=P51023-4; Sequence=VSP_059595;
CC -!- DEVELOPMENTAL STAGE: Expressed in type II neuroblasts in larval brain
CC (at protein level) (PubMed:27151950, PubMed:28245922). Expressed in a
CC complex dynamic pattern in early embryos, including the midline and
CC midline glial cells (PubMed:8223245, PubMed:1577186, PubMed:2834248).
CC Expressed throughout development with lower levels during larval
CC development (PubMed:1577186). Expressed in the eye imaginal disk in and
CC posterior to the morphogenetic furrow (PubMed:8033205). Isoform P2:
CC Detected in the precellular blastoderm stage localized at the anterior
CC tip of the embryo (PubMed:8223245). Detected during gastrulation in the
CC mesoderm (PubMed:8223245, PubMed:23757412). As the germband retracts
CC (stage 12) detected in a few cells located at the dorsal roof at the
CC midline of the CNS (PubMed:8223245). These cells correspond to the
CC midline glial cells, in which expression continues until the end of
CC embryogenesis (PubMed:8223245). Major isoform expressed in the eye
CC imaginal disk in and posterior to the morphogenetic furrow
CC (PubMed:8033205). Isoform P1: Expressed within the differentiated
CC region of the disk including and posterior to the morphogenetic furrow
CC in the proneural photoreceptor clusters (at protein level)
CC (PubMed:23757412). Detected during the cellular blastoderm stage in two
CC broad stripes in the lateral neurogenic region (PubMed:8223245). At the
CC beginning of gastrulation detected in the dorsal edge of the neurogenic
CC region (PubMed:8223245). During segmentation (stage 11), detected in
CC the ventral ectoderm and in a group of cells surrounding the future
CC tracheal pits, in the head region and the lateral body wall
CC (PubMed:8223245). In the CNS, detected during germband retraction, in
CC the glial cells and in the midline (PubMed:8223245). In larval brains,
CC major neuroblast-specific isoform (PubMed:22143802).
CC {ECO:0000269|PubMed:1577186, ECO:0000269|PubMed:22143802,
CC ECO:0000269|PubMed:23757412, ECO:0000269|PubMed:27151950,
CC ECO:0000269|PubMed:28245922, ECO:0000269|PubMed:2834248,
CC ECO:0000269|PubMed:8033205, ECO:0000269|PubMed:8223245}.
CC -!- PTM: [Isoform P2]: Phosphorylated at Thr-151 by rl.
CC {ECO:0000269|PubMed:8033205}.
CC -!- DISRUPTION PHENOTYPE: Embryonic lethal showing loss of photoreceptors
CC (PubMed:8033205, PubMed:8223245, PubMed:23757412). In larvae, RNAi-
CC mediated knockdown results in a transformation of type II neuroblasts
CC into type I neuroblasts, elimination of intermediary neuronal
CC progenitors (INP) and generation of extra type II neuroblasts; in the
CC ventral nerve cord, results in increased numbers of type I neuroblasts
CC (PubMed:27510969, PubMed:28899667, PubMed:27151950). Simultaneous RNAi-
CC mediated knockdown of the zinc-finger transcriptional repressor erm,
CC the transcriptional repressor E(spl)mdelta-HLH or the transcriptional
CC repressor dpn restore normal neuroblast numbers (PubMed:28899667).
CC Simultaneous RNAi-mediated knockdown of N partially restores normal
CC neuroblast numbers and inhibits ectopic erm expression in the central
CC brain and ventral nerve cord (PubMed:27151950).
CC {ECO:0000269|PubMed:23757412, ECO:0000269|PubMed:27151950,
CC ECO:0000269|PubMed:27510969, ECO:0000269|PubMed:28899667,
CC ECO:0000269|PubMed:8033205, ECO:0000269|PubMed:8223245}.
CC -!- SIMILARITY: Belongs to the ETS family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAN71682.1; Type=Miscellaneous discrepancy; Note=Aberrant splicing.; Evidence={ECO:0000305};
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DR EMBL; X69166; CAA48916.1; -; mRNA.
DR EMBL; X69167; CAA48917.1; -; mRNA.
DR EMBL; M88472; AAC34200.1; -; mRNA.
DR EMBL; M20408; AAA28521.1; -; Genomic_DNA.
DR EMBL; AE014297; AAF56125.1; -; Genomic_DNA.
DR EMBL; AE014297; AAN13942.1; -; Genomic_DNA.
DR EMBL; AE014297; AAN13943.1; -; Genomic_DNA.
DR EMBL; AE014297; AGB96252.1; -; Genomic_DNA.
DR EMBL; BT001893; AAN71682.1; ALT_SEQ; mRNA.
DR EMBL; BT024187; ABC86249.1; -; mRNA.
DR EMBL; BT058033; ACM16754.1; -; mRNA.
DR EMBL; BT133301; AFD10748.1; -; mRNA.
DR PIR; S33167; S33167.
DR PIR; S33168; S33168.
DR RefSeq; NP_001262872.1; NM_001275943.1. [P51023-4]
DR RefSeq; NP_524461.2; NM_079737.3. [P51023-1]
DR RefSeq; NP_732857.1; NM_170651.4. [P51023-2]
DR RefSeq; NP_732858.1; NM_170070.2. [P51023-3]
DR AlphaFoldDB; P51023; -.
DR SMR; P51023; -.
DR BioGRID; 67700; 47.
DR DIP; DIP-19576N; -.
DR IntAct; P51023; 11.
DR MINT; P51023; -.
DR STRING; 7227.FBpp0088658; -.
DR iPTMnet; P51023; -.
DR PaxDb; P51023; -.
DR DNASU; 42757; -.
DR EnsemblMetazoa; FBtr0089715; FBpp0088656; FBgn0003118. [P51023-3]
DR EnsemblMetazoa; FBtr0089716; FBpp0088657; FBgn0003118. [P51023-2]
DR EnsemblMetazoa; FBtr0089717; FBpp0088658; FBgn0003118. [P51023-1]
DR EnsemblMetazoa; FBtr0334554; FBpp0306621; FBgn0003118. [P51023-4]
DR GeneID; 42757; -.
DR KEGG; dme:Dmel_CG17077; -.
DR UCSC; CG17077-RD; d. melanogaster.
DR CTD; 42757; -.
DR FlyBase; FBgn0003118; pnt.
DR VEuPathDB; VectorBase:FBgn0003118; -.
DR eggNOG; KOG3806; Eukaryota.
DR GeneTree; ENSGT00940000166428; -.
DR HOGENOM; CLU_021277_0_0_1; -.
DR InParanoid; P51023; -.
DR OMA; LPQQTVH; -.
DR PhylomeDB; P51023; -.
DR Reactome; R-DME-2559585; Oncogene Induced Senescence.
DR SignaLink; P51023; -.
DR BioGRID-ORCS; 42757; 3 hits in 3 CRISPR screens.
DR ChiTaRS; pnt; fly.
DR GenomeRNAi; 42757; -.
DR PRO; PR:P51023; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0003118; Expressed in germ layer and 85 other tissues.
DR ExpressionAtlas; P51023; baseline and differential.
DR Genevisible; P51023; DM.
DR GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IDA:FlyBase.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:UniProtKB.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IDA:UniProtKB.
DR GO; GO:0043697; P:cell dedifferentiation; IMP:UniProtKB.
DR GO; GO:0030154; P:cell differentiation; IBA:GO_Central.
DR GO; GO:0001751; P:compound eye photoreceptor cell differentiation; IMP:FlyBase.
DR GO; GO:0008340; P:determination of adult lifespan; IGI:FlyBase.
DR GO; GO:0035225; P:determination of genital disc primordium; IMP:FlyBase.
DR GO; GO:0007173; P:epidermal growth factor receptor signaling pathway; IMP:FlyBase.
DR GO; GO:0007427; P:epithelial cell migration, open tracheal system; IMP:FlyBase.
DR GO; GO:0061331; P:epithelial cell proliferation involved in Malpighian tubule morphogenesis; IMP:FlyBase.
DR GO; GO:0007455; P:eye-antennal disc morphogenesis; IMP:FlyBase.
DR GO; GO:0008543; P:fibroblast growth factor receptor signaling pathway; IMP:FlyBase.
DR GO; GO:0007476; P:imaginal disc-derived wing morphogenesis; IMP:FlyBase.
DR GO; GO:0007479; P:leg disc proximal/distal pattern formation; IEP:FlyBase.
DR GO; GO:0035170; P:lymph gland crystal cell differentiation; IMP:FlyBase.
DR GO; GO:0035169; P:lymph gland plasmatocyte differentiation; IMP:FlyBase.
DR GO; GO:0055001; P:muscle cell development; IMP:FlyBase.
DR GO; GO:2001234; P:negative regulation of apoptotic signaling pathway; IGI:FlyBase.
DR GO; GO:0009997; P:negative regulation of cardioblast cell fate specification; IMP:FlyBase.
DR GO; GO:0043433; P:negative regulation of DNA-binding transcription factor activity; IDA:FlyBase.
DR GO; GO:0001742; P:oenocyte differentiation; IMP:FlyBase.
DR GO; GO:0016318; P:ommatidial rotation; IMP:FlyBase.
DR GO; GO:0007424; P:open tracheal system development; IMP:FlyBase.
DR GO; GO:0061320; P:pericardial nephrocyte differentiation; IMP:FlyBase.
DR GO; GO:0042461; P:photoreceptor cell development; IMP:UniProtKB.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; IMP:FlyBase.
DR GO; GO:0010628; P:positive regulation of gene expression; IDA:FlyBase.
DR GO; GO:0045666; P:positive regulation of neuron differentiation; IMP:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:FlyBase.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IMP:UniProtKB.
DR GO; GO:0007428; P:primary branching, open tracheal system; IMP:FlyBase.
DR GO; GO:0007464; P:R3/R4 cell fate commitment; IMP:FlyBase.
DR GO; GO:0007465; P:R7 cell fate commitment; IMP:FlyBase.
DR GO; GO:0090175; P:regulation of establishment of planar polarity; IMP:FlyBase.
DR GO; GO:1902692; P:regulation of neuroblast proliferation; IMP:UniProtKB.
DR GO; GO:0050767; P:regulation of neurogenesis; IMP:FlyBase.
DR GO; GO:0045664; P:regulation of neuron differentiation; IGI:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0007429; P:secondary branching, open tracheal system; IMP:FlyBase.
DR GO; GO:0045500; P:sevenless signaling pathway; IDA:FlyBase.
DR GO; GO:0035154; P:terminal cell fate specification, open tracheal system; HMP:FlyBase.
DR GO; GO:0060438; P:trachea development; IMP:FlyBase.
DR GO; GO:0048190; P:wing disc dorsal/ventral pattern formation; IGI:FlyBase.
DR DisProt; DP01749; -.
DR Gene3D; 1.10.10.10; -; 1.
DR Gene3D; 1.10.150.50; -; 1.
DR InterPro; IPR000418; Ets_dom.
DR InterPro; IPR046328; ETS_fam.
DR InterPro; IPR003118; Pointed_dom.
DR InterPro; IPR013761; SAM/pointed_sf.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR11849; PTHR11849; 2.
DR Pfam; PF00178; Ets; 1.
DR Pfam; PF02198; SAM_PNT; 1.
DR PRINTS; PR00454; ETSDOMAIN.
DR SMART; SM00413; ETS; 1.
DR SMART; SM00251; SAM_PNT; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR SUPFAM; SSF47769; SSF47769; 1.
DR PROSITE; PS00345; ETS_DOMAIN_1; 1.
DR PROSITE; PS00346; ETS_DOMAIN_2; 1.
DR PROSITE; PS50061; ETS_DOMAIN_3; 1.
DR PROSITE; PS51433; PNT; 1.
PE 1: Evidence at protein level;
KW Activator; Alternative splicing; Developmental protein; DNA-binding;
KW Nucleus; Phosphoprotein; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..718
FT /note="ETS-like protein pointed"
FT /id="PRO_0000204130"
FT DOMAIN 164..250
FT /note="PNT"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00762"
FT DNA_BIND 610..690
FT /note="ETS"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00237,
FT ECO:0000269|PubMed:1577186, ECO:0000269|PubMed:28245922,
FT ECO:0000269|PubMed:8223245"
FT REGION 303..412
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 430..521
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 545..571
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 438..455
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 485..521
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 151
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:8033205"
FT VAR_SEQ 1..323
FT /note="MELAICKTDLSATKFMLPPALPSSAAIGSSSAVASTASHFLDKAAHELFQLN
FT AINGHLFKSPASSHLNSVGSPSILSQLNGIGNSGNHSGQVSTMRLKKNRKVTFLSSLVE
FT SKNIFIKEEPIHGCKDLCSLSDISDHEASLEVPTALPPLTPGTNRKVNEVLKASFASWE
FT KEVQKCNITKDPREWTEEHVIYWLNWAKNEFSLVSMNLDPFYKMKGRAMVDLGKEKFLA
FT ITPPFTGDILWEHLDILQKDCEKPNEDIVHGNSFESATTASVCGSDHQVANYPNETHAN
FT SNNSSINSRLSMDYVTSAGNSDNKNFHRATPHSHN -> MPPSAFLVNASIISAANALD
FT SDKEQQLQEHLNQSHFTSSSSSSNSSNNNNCHTAPNYNMVLQSYENYPSYHLAQHHHHH
FT HHHHSHPHHQLAQQTGLHSHHTMQQQLQQQQQSLLLQHPQQQQQQHSHQSQQQQQHGYG
FT SSAQLPHHRLSGGSTGSTTTTSSGSSSSGSSSSASSQGFANGSATANNLVGALSSSTAA
FT SLGLGYFNDMAPFVGDANAYYTDSDVNFFSSGT (in isoform E)"
FT /evidence="ECO:0000305"
FT /id="VSP_059595"
FT VAR_SEQ 1..323
FT /note="MELAICKTDLSATKFMLPPALPSSAAIGSSSAVASTASHFLDKAAHELFQLN
FT AINGHLFKSPASSHLNSVGSPSILSQLNGIGNSGNHSGQVSTMRLKKNRKVTFLSSLVE
FT SKNIFIKEEPIHGCKDLCSLSDISDHEASLEVPTALPPLTPGTNRKVNEVLKASFASWE
FT KEVQKCNITKDPREWTEEHVIYWLNWAKNEFSLVSMNLDPFYKMKGRAMVDLGKEKFLA
FT ITPPFTGDILWEHLDILQKDCEKPNEDIVHGNSFESATTASVCGSDHQVANYPNETHAN
FT SNNSSINSRLSMDYVTSAGNSDNKNFHRATPHSHN -> MPPSAFLVNASIISAANALD
FT SDKEQQLQEHLNQSHFTSSSSSSNSSNNNNCHTAPNYNMVLQSYENYPSYHLAQHHHHH
FT HHHHSHPHHQLAQQTGLHSHHTMQQQLQQQQQSLLLQHPQQQQQQHSHQSQQQQQHGYG
FT SSAQLPHHRLSGGSTGSTTTTSSGSSSSGSSSSASSQGFANGSATANNLVGALSSSTAA
FT SLGLGYFNDMAPFVGDANAYYTDSDVNFFSS (in isoform P1)"
FT /evidence="ECO:0000305"
FT /id="VSP_059596"
FT VAR_SEQ 1..141
FT /note="MELAICKTDLSATKFMLPPALPSSAAIGSSSAVASTASHFLDKAAHELFQLN
FT AINGHLFKSPASSHLNSVGSPSILSQLNGIGNSGNHSGQVSTMRLKKNRKVTFLSSLVE
FT SKNIFIKEEPIHGCKDLCSLSDISDHEASL -> MTNEWIDWNDSRMLPPLRSANYNYH
FT PQTFLPNNYQCFTGKFHLKGQKLQQLTTNHSKLK (in isoform D)"
FT /evidence="ECO:0000305"
FT /id="VSP_059597"
FT MUTAGEN 151
FT /note="T->A: Reduced activity and lack of response to
FT Ras85D or rl stimulation."
FT /evidence="ECO:0000269|PubMed:8033205"
FT CONFLICT 133..135
FT /note="DIS -> VYP (in Ref. 1; CAA48917)"
FT /evidence="ECO:0000305"
FT CONFLICT 420
FT /note="A -> AM (in Ref. 1; CAA48917)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 718 AA; 77683 MW; FD6AFD0F4BCD69C5 CRC64;
MELAICKTDL SATKFMLPPA LPSSAAIGSS SAVASTASHF LDKAAHELFQ LNAINGHLFK
SPASSHLNSV GSPSILSQLN GIGNSGNHSG QVSTMRLKKN RKVTFLSSLV ESKNIFIKEE
PIHGCKDLCS LSDISDHEAS LEVPTALPPL TPGTNRKVNE VLKASFASWE KEVQKCNITK
DPREWTEEHV IYWLNWAKNE FSLVSMNLDP FYKMKGRAMV DLGKEKFLAI TPPFTGDILW
EHLDILQKDC EKPNEDIVHG NSFESATTAS VCGSDHQVAN YPNETHANSN NSSINSRLSM
DYVTSAGNSD NKNFHRATPH SHNGYNTSNT HDRINNSTPP QQQQSQQPTV NGSGSASSNN
NNSMLPPAVQ QSNNENNNTS SSNTNNSSNN NNNSGGSNNS NAGSNNNNNN NNNINFMAAA
AIFQHHLKEE PGTQNGNIGG YGGGSNSQND PTDLSSYGLP AHLAAYGGGS GSGPTGGRSS
GGGGDESDYH STISAQDHQS QQSSGGNGSG GASGGSTGNS NGYLDSSSEF YGSYAGRNRF
HDGYPPEFTP YDAQSFQSMG PQPTAMDQWG AAHAHQHPAA YMSTLGLDKG LLGGYTTQGG
VPCFTGSGPI QLWQFLLELL LDKTCQSFIS WTGDGWEFKL TDPDEVARRW GIRKNKPKMN
YEKLSRGLRY YYDKNIIHKT AGKRYVYRFV CDLQNLVGHT PEELVAKYDL KIEKKDVD
