PNUC_SALTY
ID PNUC_SALTY Reviewed; 239 AA.
AC P24520;
DT 01-MAR-1992, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2002, sequence version 2.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=Nicotinamide riboside transporter PnuC;
GN Name=pnuC; OrderedLocusNames=STM0757;
OS Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Salmonella.
OX NCBI_TaxID=99287;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2198247; DOI=10.1128/jb.172.8.4187-4196.1990;
RA Foster J.W., Park Y.K., Penfound T., Fenger T., Spector M.P.;
RT "Regulation of NAD metabolism in Salmonella typhimurium: molecular sequence
RT analysis of the bifunctional nadR regulator and the nadA-pnuC operon.";
RL J. Bacteriol. 172:4187-4196(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX PubMed=11677609; DOI=10.1038/35101614;
RA McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA Wilson R.K.;
RT "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL Nature 413:852-856(2001).
CC -!- FUNCTION: Required for nicotinamide riboside transport across the inner
CC membrane. {ECO:0000250|UniProtKB:Q57425}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000250|UniProtKB:Q57425}; Multi-pass membrane protein
CC {ECO:0000250|UniProtKB:Q57425}.
CC -!- INDUCTION: Repressed by NadR.
CC -!- SIMILARITY: Belongs to the nicotinamide ribonucleoside (NR) uptake
CC permease (TC 4.B.1) family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=M85180; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; M85180; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AE006468; AAL19696.1; -; Genomic_DNA.
DR PIR; D37753; D37753.
DR RefSeq; NP_459737.1; NC_003197.2.
DR RefSeq; WP_000345368.1; NC_003197.2.
DR AlphaFoldDB; P24520; -.
DR SMR; P24520; -.
DR STRING; 99287.STM0757; -.
DR TCDB; 4.B.1.1.1; the nicotinamide ribonucleoside (nr) uptake permease (pnuc) family.
DR PaxDb; P24520; -.
DR PRIDE; P24520; -.
DR EnsemblBacteria; AAL19696; AAL19696; STM0757.
DR GeneID; 1252277; -.
DR KEGG; stm:STM0757; -.
DR PATRIC; fig|99287.12.peg.788; -.
DR HOGENOM; CLU_076589_1_0_6; -.
DR OMA; WPDAYIF; -.
DR PhylomeDB; P24520; -.
DR BioCyc; SENT99287:STM0757-MON; -.
DR Proteomes; UP000001014; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0034257; F:nicotinamide riboside transmembrane transporter activity; IEA:InterPro.
DR InterPro; IPR006419; NMN_transpt_PnuC.
DR PANTHER; PTHR36122; PTHR36122; 1.
DR Pfam; PF04973; NMN_transporter; 1.
DR TIGRFAMs; TIGR01528; NMN_trans_PnuC; 1.
PE 2: Evidence at transcript level;
KW Cell inner membrane; Cell membrane; Membrane; NAD; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..239
FT /note="Nicotinamide riboside transporter PnuC"
FT /id="PRO_0000058490"
FT TOPO_DOM 1..21
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 22..42
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 43..48
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 49..68
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 69..71
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 72..89
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 90..109
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 110..127
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 128..157
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 158..177
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 178..183
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 184..206
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 207..239
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT BINDING 188
FT /ligand="beta-nicotinamide D-riboside"
FT /ligand_id="ChEBI:CHEBI:15927"
FT /evidence="ECO:0000250|UniProtKB:D2ZZC1"
FT BINDING 192
FT /ligand="beta-nicotinamide D-riboside"
FT /ligand_id="ChEBI:CHEBI:15927"
FT /evidence="ECO:0000250|UniProtKB:D2ZZC1"
FT CONFLICT 71
FT /note="L -> R (in Ref. 1)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 239 AA; 27191 MW; D3A35FE6D952AF86 CRC64;
MDFFSTHNIL IHIPIGAGGY DLSWIEAVGT IAGLLCIWLA SLEKISNYFF GLVNVTLFAI
IFFQIQLYAS LLLQLFFFAA NIYGWYAWSR QTKDNQAELK IRWLPLPKAM AWLAICVIAI
GLMTRYIDPV FAVLTRVAVA IMQMLGLQVT MPVLQPDAFP FWDSCMMVLS IVAMILMTRK
YVENWLLWVI INVISVVIFA LQGVYAMSLE YLILTFIAVN GSRLWINSAR ERGSRALSR
