PNX_DANRE
ID PNX_DANRE Reviewed; 182 AA.
AC F1R2J1; Q8AXR6;
DT 25-OCT-2017, integrated into UniProtKB/Swiss-Prot.
DT 03-MAY-2011, sequence version 1.
DT 03-AUG-2022, entry version 72.
DE RecName: Full=Homeobox protein pnx {ECO:0000303|PubMed:12642490};
DE AltName: Full=Posterior neuron-specific homeobox {ECO:0000303|PubMed:12642490};
GN Name=pnx {ECO:0000303|PubMed:12642490};
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955 {ECO:0000312|Proteomes:UP000000437};
RN [1] {ECO:0000312|EMBL:BAC54031.1}
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH TLE3A, SUBCELLULAR
RP LOCATION, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=12642490; DOI=10.1242/dev.00418;
RA Bae Y.B., Shimizu T., Yabe T., Kim C., Hirata T., Nojima H., Muraoka O.,
RA Hirano T., Hibi M.;
RT "A homeobox gene, pnx, is involved in the formation of posterior neurons in
RT zebrafish.";
RL Development 130:1853-1865(2003).
RN [2] {ECO:0000312|Proteomes:UP000000437}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen {ECO:0000312|Proteomes:UP000000437};
RX PubMed=23594743; DOI=10.1038/nature12111;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [3] {ECO:0000312|EMBL:AAI62069.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Transcriptional repressor. Activity as a repressor is
CC enhanced by binding to the corepressor tle3a.
CC {ECO:0000269|PubMed:12642490}.
CC -!- SUBUNIT: Interacts with tle3a. {ECO:0000269|PubMed:12642490}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255, ECO:0000255|PROSITE-
CC ProRule:PRU00108, ECO:0000255|RuleBase:RU000682,
CC ECO:0000269|PubMed:12642490}.
CC -!- DEVELOPMENTAL STAGE: Detected in the presumptive ectoderm at the late
CC blastula stage. During the gastrula stage, expression refines to the
CC ventrolateral ectoderm and the dorsal-midline ectoderm. Expressed in
CC primary neurons during the segmentation stage, with strongest
CC expression in primary motoneurons and interneurons and lower expression
CC in Rohon-Beard neurons. The expression domain is restricted to regions
CC posterior of rhombomere 4. During the late segmentation and pharyngula
CC stages, expression decreases in an anterior-to-posterior direction and
CC is largely absent by 48 hours post-fertilization.
CC {ECO:0000269|PubMed:12642490}.
CC -!- DISRUPTION PHENOTYPE: Morpholino knockdown of the protein results in
CC impaired neurogenesis in posterior regions. Expression of the neural
CC precursor marker ngn1 is reduced at the one- to three-somite stage. At
CC later stages, there is a significant reduction in islet1-expressing
CC primary motoneurons and defective axon outgrowth from motoneurons.
CC Formation of Rohon-Beard neurons is initially abnormal but later
CC recovers. Development of secondary neurons is not affected.
CC {ECO:0000269|PubMed:12642490}.
CC -!- SIMILARITY: Belongs to the NK-1 homeobox family. {ECO:0000305}.
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DR EMBL; AB067731; BAC54031.1; -; mRNA.
DR EMBL; CT027796; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC162069; AAI62069.1; -; mRNA.
DR EMBL; BC162454; AAI62454.1; -; mRNA.
DR RefSeq; NP_840087.1; NM_178302.2.
DR AlphaFoldDB; F1R2J1; -.
DR SMR; F1R2J1; -.
DR STRING; 7955.ENSDARP00000034835; -.
DR PaxDb; F1R2J1; -.
DR Ensembl; ENSDART00000030709; ENSDARP00000034835; ENSDARG00000025899.
DR GeneID; 352939; -.
DR KEGG; dre:352939; -.
DR CTD; 352939; -.
DR ZFIN; ZDB-GENE-030328-42; pnx.
DR eggNOG; KOG0489; Eukaryota.
DR GeneTree; ENSGT00940000166967; -.
DR HOGENOM; CLU_127172_0_0_1; -.
DR InParanoid; F1R2J1; -.
DR OMA; FERHCIA; -.
DR OrthoDB; 1462635at2759; -.
DR PhylomeDB; F1R2J1; -.
DR TreeFam; TF315720; -.
DR PRO; PR:F1R2J1; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 10.
DR Bgee; ENSDARG00000025899; Expressed in gastrula and 9 other tissues.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0030154; P:cell differentiation; IBA:GO_Central.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IDA:ZFIN.
DR GO; GO:0007399; P:nervous system development; IEA:UniProtKB-KW.
DR GO; GO:0050767; P:regulation of neurogenesis; IMP:ZFIN.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR020479; Homeobox_metazoa.
DR InterPro; IPR000047; HTH_motif.
DR Pfam; PF00046; Homeodomain; 1.
DR PRINTS; PR00024; HOMEOBOX.
DR PRINTS; PR00031; HTHREPRESSR.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 1: Evidence at protein level;
KW Developmental protein; DNA-binding; Homeobox; Neurogenesis; Nucleus;
KW Reference proteome; Repressor; Transcription; Transcription regulation.
FT CHAIN 1..182
FT /note="Homeobox protein pnx"
FT /id="PRO_0000442136"
FT DNA_BIND 67..126
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 1..34
FT /note="Important for interaction with tle3a"
FT /evidence="ECO:0000269|PubMed:12642490"
FT REGION 24..63
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 40..57
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 51
FT /note="D -> E (in Ref. 1; BAC54031 and 3; AAI62069/
FT AAI62454)"
FT /evidence="ECO:0000305"
FT CONFLICT 178
FT /note="T -> M (in Ref. 1; BAC54031 and 3; AAI62069/
FT AAI62454)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 182 AA; 20704 MW; 6BBE5DDC517BBEAA CRC64;
MHEETSNSTL QGKTSFSIAD ILDPAKFNGT RETREISNNR ESPKTTSPTQ DPSAPNIANA
SAAKVKSKRI RTAFTLDQLR ILERSFQSSH YLSVFERHCI ASALGLSETQ VKIWFQNRRT
KWKKELDGHG GEEQSHCAPT ALTQNPIMYA LPGHHANHHV HYYPQQTHYL NTSFHPQTLM
MY
