ID   PO21_BRACO              Reviewed;         869 AA.
AC   Q03279;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1994, sequence version 1.
DT   25-MAY-2022, entry version 64.
DE   RecName: Full=Retrovirus-related Pol polyprotein from type-1 retrotransposable element R2;
DE   AltName: Full=Retrovirus-related Pol polyprotein from type I retrotransposable element R2;
DE   Includes:
DE     RecName: Full=Reverse transcriptase;
DE              EC=2.7.7.49;
DE   Includes:
DE     RecName: Full=Endonuclease;
DE   Flags: Fragment;
OS   Bradysia coprophila (Dark-winged fungus gnat) (Sciara coprophila).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Nematocera; Sciaroidea; Sciaridae;
OC   Bradysia.
OX   NCBI_TaxID=38358;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8383793; DOI=10.1093/oxfordjournals.molbev.a039990;
RA   Burke W.D., Eickbush D.G., Xiong Y., Jakubczak J.L., Eickbush T.H.;
RT   "Sequence relationship of retrotransposable elements R1 and R2 within and
RT   between divergent insect species.";
RL   Mol. Biol. Evol. 10:163-185(1993).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.49; Evidence={ECO:0000255|PROSITE-
CC         ProRule:PRU00405};
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DR   EMBL; L00951; AAA29814.1; -; Genomic_DNA.
DR   PIR; G44490; G44490.
DR   AlphaFoldDB; Q03279; -.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0003964; F:RNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR000477; RT_dom.
DR   Pfam; PF00078; RVT_1; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS50878; RT_POL; 1.
PE   4: Predicted;
KW   Endonuclease; Hydrolase; Nuclease; Nucleotidyltransferase;
KW   RNA-directed DNA polymerase; Transferase; Transposable element.
FT   CHAIN           <1..869
FT                   /note="Retrovirus-related Pol polyprotein from type-1
FT                   retrotransposable element R2"
FT                   /id="PRO_0000058499"
FT   DOMAIN          199..475
FT                   /note="Reverse transcriptase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00405"
FT   REGION          601..869
FT                   /note="Nucleic acid-binding endonuclease"
FT   NON_TER         1
SQ   SEQUENCE   869 AA;  97399 MW;  9584756BFA7AF818 CRC64;
     VSVPPTSYRD RIMNALEESM IDVDAIRGSS ARELVEIGKV ALLNQPMDEG RIMSWLSDRF
     PDTQKPKGPI YSKTTIYHGT NKQKRKQRYA LVQSLYKKDI SAAARVVLDE NDKIATKIPP
     VRHMFDYWKD VFATGGGSAA TNINRAPPAP HMETLWDPVS LIEIKSARAS NEKGAGPDGV
     TPRSWNALDD RYKRLLYNIF VFYGRVPSPI KGSRTVFTPK IEGGPDPGVF RPLSICSVIL
     REFNKILARR FVSCYTYDER QTAYLPIDGV CINVSMLTAI IAEAKRLRKE LHIAILDLVK
     AFNSVYHSAL IDAITEAGCP PGVVDYIADM YNNVITEMQF EGKCELASIL AGVYQGDPLS
     GPLFTLAYEK ALRALNNEGR FDIADVRVNA SAYSDDGLLL AMTVIGLQHN LDKFGETLAK
     IGLRINSRKS KTVSLVPSGR EKKMKIVSNR RLLLKASELK PLTISDLWKY LGVVYTTSGP
     EVAKVSMDDD LSKLTKGPLK PQQRIHLLKT FVIPKHLNRL VLSRTTATGL CKMDLLIRKY
     VRRWLRLPGD VPVAFLYAPV KAGGKGIPCL KQWIPLMRFL RLNKAKRTGG DRIAAVLNCQ
     LYASISHSCK TGPVSVGLWR STNTGGLSAY WRRILIGMVD GKDLKSAQNH SSATSFNSIR
     MNDISGEDYI HYNQLRTNSI PTRKRTARGR PNKPTACRAG CDKLKRLQHD IQGCIRSQGG
     LVQRHDRVVD LLFDECETKG YAAEKVVHLR TSEELWKPDL VLKKNGRVVV VDAQVVQCGR
     LESDHRVKVS KYRDDPELAD VIREKYAVQE VTFEACTLSY KGIWSKNSVE GLQKLGISNY
     CLFKIVTSVL RGSWLNWVRF NNVTTVVHW