ID   PO2F1_XENTR             Reviewed;         757 AA.
AC   Q28BL7;
DT   15-DEC-2009, integrated into UniProtKB/Swiss-Prot.
DT   04-APR-2006, sequence version 1.
DT   03-AUG-2022, entry version 106.
DE   RecName: Full=POU domain, class 2, transcription factor 1 {ECO:0000312|EMBL:CAJ82126.1};
DE   AltName: Full=Octamer-binding protein 1;
DE            Short=Oct-1 {ECO:0000250|UniProtKB:P16143};
DE   AltName: Full=Octamer-binding transcription factor 1 {ECO:0000250|UniProtKB:P16143};
DE            Short=OTF-1;
GN   Name=pou2f1 {ECO:0000312|EMBL:CAJ82126.1};
GN   Synonyms=oct-1 {ECO:0000250|UniProtKB:P16143},
GN   oct1 {ECO:0000250|UniProtKB:P16143}; ORFNames=TEgg001p15.1;
OS   Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX   NCBI_TaxID=8364;
RN   [1] {ECO:0000312|EMBL:CAJ82126.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Egg {ECO:0000312|EMBL:CAJ82126.1};
RG   Sanger Xenopus tropicalis EST/cDNA project;
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Transcription factor that binds to the octamer motif (5'-
CC       ATTTGCAT-3') and activates the promoters of the genes for some small
CC       nuclear RNAs (snRNA) and histone H2B. Acts downstream of Notch
CC       signaling during radial glia formation. Regulates apoptosis, possibly
CC       via an FGF-signaling pathway (By similarity).
CC       {ECO:0000250|UniProtKB:P16143}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:P16143}. Nucleus
CC       {ECO:0000250|UniProtKB:P16143, ECO:0000255|PROSITE-ProRule:PRU00108,
CC       ECO:0000255|PROSITE-ProRule:PRU00530}. Note=Retained in the cytoplasm
CC       during early development, then gradually translocates to the nucleus
CC       around the mid-blastula transition (MBT).
CC       {ECO:0000250|UniProtKB:P16143}.
CC   -!- DOMAIN: The POU domain controls nuclear translocation.
CC       {ECO:0000250|UniProtKB:P16143}.
CC   -!- SIMILARITY: Belongs to the POU transcription factor family. Class-2
CC       subfamily. {ECO:0000255}.
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DR   EMBL; CR942771; CAJ82126.1; -; mRNA.
DR   RefSeq; NP_001016337.2; NM_001016337.3.
DR   RefSeq; XP_012812409.1; XM_012956955.2.
DR   AlphaFoldDB; Q28BL7; -.
DR   SMR; Q28BL7; -.
DR   STRING; 8364.ENSXETP00000033002; -.
DR   PaxDb; Q28BL7; -.
DR   GeneID; 549091; -.
DR   KEGG; xtr:549091; -.
DR   CTD; 5451; -.
DR   Xenbase; XB-GENE-853992; pou2f1.
DR   eggNOG; KOG3802; Eukaryota.
DR   HOGENOM; CLU_013065_4_0_1; -.
DR   InParanoid; Q28BL7; -.
DR   OrthoDB; 873012at2759; -.
DR   Reactome; R-XTR-6807505; RNA polymerase II transcribes snRNA genes.
DR   Proteomes; UP000008143; Chromosome 2.
DR   Proteomes; UP000790000; Unplaced.
DR   Bgee; ENSXETG00000006231; Expressed in 4-cell stage embryo and 14 other tissues.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0005667; C:transcription regulator complex; ISS:UniProtKB.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0043565; F:sequence-specific DNA binding; ISS:UniProtKB.
DR   GO; GO:0007219; P:Notch signaling pathway; ISS:UniProtKB.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR   GO; GO:0045893; P:positive regulation of transcription, DNA-templated; ISS:UniProtKB.
DR   GO; GO:0060019; P:radial glial cell differentiation; ISS:UniProtKB.
DR   GO; GO:0042981; P:regulation of apoptotic process; ISS:UniProtKB.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   CDD; cd00086; homeodomain; 1.
DR   Gene3D; 1.10.260.40; -; 1.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR017970; Homeobox_CS.
DR   InterPro; IPR001356; Homeobox_dom.
DR   InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR   InterPro; IPR013847; POU.
DR   InterPro; IPR045703; POU2F1_C.
DR   InterPro; IPR000327; POU_dom.
DR   InterPro; IPR000972; TF_octamer.
DR   Pfam; PF00046; Homeodomain; 1.
DR   Pfam; PF00157; Pou; 1.
DR   Pfam; PF19536; POU2F1_C; 1.
DR   PRINTS; PR00029; OCTAMER.
DR   PRINTS; PR00028; POUDOMAIN.
DR   SMART; SM00389; HOX; 1.
DR   SMART; SM00352; POU; 1.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   SUPFAM; SSF47413; SSF47413; 1.
DR   PROSITE; PS00027; HOMEOBOX_1; 1.
DR   PROSITE; PS50071; HOMEOBOX_2; 1.
DR   PROSITE; PS00035; POU_1; 1.
DR   PROSITE; PS00465; POU_2; 1.
DR   PROSITE; PS51179; POU_3; 1.
PE   2: Evidence at transcript level;
KW   Activator; Cytoplasm; Developmental protein; DNA-binding; Homeobox;
KW   Nucleus; Reference proteome; Transcription; Transcription regulation.
FT   CHAIN           1..757
FT                   /note="POU domain, class 2, transcription factor 1"
FT                   /id="PRO_0000389618"
FT   DOMAIN          294..368
FT                   /note="POU-specific"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00530"
FT   DNA_BIND        395..454
FT                   /note="Homeobox"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT   REGION          1..43
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          271..295
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          375..398
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          532..574
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        271..290
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   757 AA;  78444 MW;  65AD8A5AF51B02E2 CRC64;
     MKLHSSSKIP NHAWLSDARM NNPSETSKSS ESGDGNTGTQ TNGLDFQKQA VPIGAITSAQ
     AQALLGHLHQ VQLAGTSLQA AAQSLNVQTK FKEEPGEPTQ AVQPSQQPSL QAAIPQTQLM
     VAGGPITGLT LTPAQQQLLL QQAQAQLLAA AVQHSASQQH SAAGATISAS AATPMTQIPL
     SQPIQIAQDL QQLQQLQQQN LNLQQFVLVH PTTNLPPAQF IISQTPQGQQ GLLQAQNLLT
     QLPQQSQANL LQSQPSITLT SQPATPTRTI AATPVQQLPQ SQTTPKRIDT PSLEEPSDLE
     ELEQFAKTFK QRRIKLGFTQ GDVGLAMGKL YGNDFSQTTI SRFEALNLSF KNMCKLKPLL
     EKWLNDAENI TSDSSLSNQS VLNSPGHGME GLNRRRKKRT SIETNIRVAL EKSFLENQKP
     TSEEITMIAD QLNMEKEVIR VWFCNRRQKE KRINPPSSGG SSSSPIKSLF SSANPLVATT
     PSLVTSSTAT TLTVNPVLPL TSAAAITSFP VPGTTGTSSA NTATVISTAP PVSSVLTSPS
     LSPSPSATAA TSEASTASGT STTHTTSTPL TSPLSTGQVM VTASGIHTAA TALQGAAQLP
     TSASLAAMAA AAGLNPGLMA PSQFAAGGAL FSLNPGALGS ALSPALMSNS TLATIQALAS
     SGSLPITSLD ATGNLVFANA GGTPNIVTAP LFLNPQNLSL LTSNPVSLVS AASTGVTGPI
     TSLHATTSSV DSVQNTLFTV ASASGAASTT TSASKAQ