PO2F2_PIG
ID PO2F2_PIG Reviewed; 478 AA.
AC Q29013; Q29089;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 25-MAY-2022, entry version 130.
DE RecName: Full=POU domain, class 2, transcription factor 2;
DE AltName: Full=Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2;
DE AltName: Full=Octamer-binding protein 2;
DE Short=Oct-2;
DE AltName: Full=Octamer-binding transcription factor 2;
DE Short=OTF-2;
GN Name=POU2F2; Synonyms=OCT2, OTF2;
OS Sus scrofa (Pig).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Suina; Suidae; Sus.
OX NCBI_TaxID=9823;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Spleen;
RX PubMed=7943947; DOI=10.1111/j.1365-2052.1994.tb00102.x;
RA Tuggle C.K., Helm J., Rothschild M.F.;
RT "Cloning, sequencing and restriction fragment length polymorphism analysis
RT of a porcine cDNA for OCT2.";
RL Anim. Genet. 25:141-145(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 210-350.
RC STRAIN=Duroc;
RX PubMed=8270542; DOI=10.2527/1993.71113172x;
RA Tuggle C.K.;
RT "Cloning and sequence analysis of the swine Oct-2 POU-domain genomic
RT region.";
RL J. Anim. Sci. 71:3172-3172(1993).
CC -!- FUNCTION: Transcription factor that specifically binds to the octamer
CC motif (5'-ATTTGCAT-3'). Regulates IL6 expression in B cells with
CC POU2AF1. Regulates transcription in a number of tissues in addition to
CC activating immunoglobulin gene expression. Modulates transcription
CC transactivation by NR3C1, AR and PGR. {ECO:0000250|UniProtKB:P09086,
CC ECO:0000250|UniProtKB:Q00196}.
CC -!- ACTIVITY REGULATION: Transactivation activity is enhanced by
CC transcriptional coactivator POU2AF1. {ECO:0000250|UniProtKB:Q00196}.
CC -!- SUBUNIT: Interacts with NR3C1, AR and PGR. Interacts with POU2AF1; the
CC interaction increases POU2F2 transactivation activity.
CC {ECO:0000250|UniProtKB:P09086}.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- TISSUE SPECIFICITY: Predominantly expressed in B-cells.
CC -!- SIMILARITY: Belongs to the POU transcription factor family. Class-2
CC subfamily. {ECO:0000305}.
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DR EMBL; U00794; AAA80148.1; -; mRNA.
DR EMBL; L03842; AAA74657.1; -; Genomic_DNA.
DR PIR; I47154; I47154.
DR RefSeq; NP_999205.1; NM_214040.1.
DR AlphaFoldDB; Q29013; -.
DR SMR; Q29013; -.
DR PRIDE; Q29013; -.
DR GeneID; 397105; -.
DR KEGG; ssc:397105; -.
DR CTD; 5452; -.
DR InParanoid; Q29013; -.
DR ChiTaRS; SLC22A2; pig.
DR Proteomes; UP000008227; Unplaced.
DR Proteomes; UP000314985; Unplaced.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:UniProtKB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; ISS:UniProtKB.
DR GO; GO:0098586; P:cellular response to virus; ISS:UniProtKB.
DR GO; GO:0032755; P:positive regulation of interleukin-6 production; ISS:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd00086; homeodomain; 1.
DR Gene3D; 1.10.260.40; -; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR InterPro; IPR013847; POU.
DR InterPro; IPR000327; POU_dom.
DR InterPro; IPR000972; TF_octamer.
DR Pfam; PF00046; Homeodomain; 1.
DR Pfam; PF00157; Pou; 1.
DR PRINTS; PR00029; OCTAMER.
DR PRINTS; PR00028; POUDOMAIN.
DR SMART; SM00389; HOX; 1.
DR SMART; SM00352; POU; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR SUPFAM; SSF47413; SSF47413; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
DR PROSITE; PS00035; POU_1; 1.
DR PROSITE; PS00465; POU_2; 1.
DR PROSITE; PS51179; POU_3; 1.
PE 2: Evidence at transcript level;
KW Activator; DNA-binding; Homeobox; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..478
FT /note="POU domain, class 2, transcription factor 2"
FT /id="PRO_0000100716"
FT DOMAIN 195..269
FT /note="POU-specific"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00530"
FT DNA_BIND 297..356
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 1..82
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 167..199
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 275..298
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 357..391
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 389..410
FT /note="Leucine-zipper"
FT REGION 409..478
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 14..37
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 38..62
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 431..445
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 446..472
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 212
FT /note="Q -> V (in Ref. 2; AAA74657)"
FT /evidence="ECO:0000305"
FT CONFLICT 215
FT /note="I -> M (in Ref. 2; AAA74657)"
FT /evidence="ECO:0000305"
FT CONFLICT 222
FT /note="G -> V (in Ref. 2; AAA74657)"
FT /evidence="ECO:0000305"
FT CONFLICT 233
FT /note="G -> A (in Ref. 2; AAA74657)"
FT /evidence="ECO:0000305"
FT CONFLICT 238
FT /note="Q -> K (in Ref. 2; AAA74657)"
FT /evidence="ECO:0000305"
FT CONFLICT 285
FT /note="R -> S (in Ref. 2; AAA74657)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 478 AA; 51099 MW; FD196758B603B718 CRC64;
MVHSSMGAPE IRMSKPLEAE KQGLDSPSEH TDTERNGPDT NHQNPQNKTS PFSVSPTGPS
TKIKAEDPSG DSAPAGPPPP QAVQAHLSQV QLMLTGRQLA GDIQQILQLQ QLVLVPGHHL
QPPAQFLLPQ AQQSQPGLLP TPNLFQLPQQ TQGALLTSQP RAGLPTQAVT RPTLSDPHLS
HPQPPKCLEP PSHPEEASDL EELEQFARTF KQRRIKLGFT QGDVGLAMGK LYGNDFSQTT
ISRFEALNLS FKNMCKLKPL LEKWLNDAET MSVDSSLPSP NQLSRPSLGF DGLPGRRRKK
RTSIETNVRF ALEKSFLANQ KPTSEEILLI AEQLHMEKEV IRVWFCNRRQ KEKRINPCSA
APMLPSPGKP ASYSPHLVTP QGGAGTLPLS QASSSLSTTV TTLSSAVGTL HPSRTAGGGA
AGGGAAPPLN SIPSVTPPPP ATTNSTNPSP QGSHSAIGLS GLNPSTGPGL WNPAPYQP
