PO3F1_HUMAN
ID PO3F1_HUMAN Reviewed; 451 AA.
AC Q03052; Q5TAG2;
DT 01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT 16-DEC-2008, sequence version 3.
DT 03-AUG-2022, entry version 191.
DE RecName: Full=POU domain, class 3, transcription factor 1;
DE AltName: Full=Octamer-binding protein 6;
DE Short=Oct-6;
DE AltName: Full=Octamer-binding transcription factor 6;
DE Short=OTF-6;
DE AltName: Full=POU domain transcription factor SCIP;
GN Name=POU3F1; Synonyms=OCT6, OTF6;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=7909356; DOI=10.1128/mcb.14.5.3263-3275.1994;
RA Faus I., Hsu H.J., Fuchs E.;
RT "Oct-6: a regulator of keratinocyte gene expression in stratified squamous
RT epithelia.";
RL Mol. Cell. Biol. 14:3263-3275(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16710414; DOI=10.1038/nature04727;
RA Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A.,
RA Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C.,
RA Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.,
RA Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C.,
RA Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W.,
RA Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J.,
RA Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J.,
RA Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y.,
RA Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J.,
RA Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H.,
RA Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L.,
RA Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J.,
RA Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S.,
RA Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K.,
RA Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R.,
RA Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M.,
RA Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S.,
RA Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J.,
RA Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W.,
RA McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N.,
RA Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V.,
RA Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J.,
RA Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E.,
RA Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S.,
RA Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M.,
RA White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H.,
RA Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E.,
RA Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G.,
RA Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.;
RT "The DNA sequence and biological annotation of human chromosome 1.";
RL Nature 441:315-321(2006).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 53-451.
RX PubMed=8451175; DOI=10.1093/nar/21.4.1043;
RA Tobler A., Schreiber E., Fontana A.;
RT "The human Oct-6 POU transcription factor lacks the first 50 amino acids of
RT its murine counterpart.";
RL Nucleic Acids Res. 21:1043-1043(1993).
CC -!- FUNCTION: Transcription factor that binds to the octamer motif (5'-
CC ATTTGCAT-3') (By similarity). Acts as a transcriptional activator when
CC binding cooperatively with SOX4, SOX11, or SOX12 to gene promoters (By
CC similarity). Acts as a transcriptional repressor of myelin-specific
CC genes (By similarity). {ECO:0000250|UniProtKB:P20267,
CC ECO:0000250|UniProtKB:P21952}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:P21952}.
CC -!- TISSUE SPECIFICITY: Expressed in embryonal stem cells and in the
CC developing brain.
CC -!- SIMILARITY: Belongs to the POU transcription factor family. Class-3
CC subfamily. {ECO:0000305}.
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DR EMBL; L26494; AAA59965.1; -; mRNA.
DR EMBL; AL139158; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471059; EAX07298.1; -; Genomic_DNA.
DR EMBL; Z18284; CAA79158.1; -; mRNA.
DR CCDS; CCDS30679.1; -.
DR PIR; A56018; A56018.
DR RefSeq; NP_002690.3; NM_002699.3.
DR AlphaFoldDB; Q03052; -.
DR SMR; Q03052; -.
DR BioGRID; 111449; 27.
DR DIP; DIP-277N; -.
DR IntAct; Q03052; 3.
DR STRING; 9606.ENSP00000362103; -.
DR iPTMnet; Q03052; -.
DR PhosphoSitePlus; Q03052; -.
DR BioMuta; POU3F1; -.
DR DMDM; 218512119; -.
DR EPD; Q03052; -.
DR jPOST; Q03052; -.
DR MassIVE; Q03052; -.
DR MaxQB; Q03052; -.
DR PaxDb; Q03052; -.
DR PeptideAtlas; Q03052; -.
DR PRIDE; Q03052; -.
DR ProteomicsDB; 58162; -.
DR Antibodypedia; 17662; 273 antibodies from 33 providers.
DR DNASU; 5453; -.
DR Ensembl; ENST00000373012.5; ENSP00000362103.2; ENSG00000185668.8.
DR GeneID; 5453; -.
DR KEGG; hsa:5453; -.
DR MANE-Select; ENST00000373012.5; ENSP00000362103.2; NM_002699.4; NP_002690.3.
DR UCSC; uc001ccp.2; human.
DR CTD; 5453; -.
DR DisGeNET; 5453; -.
DR GeneCards; POU3F1; -.
DR HGNC; HGNC:9214; POU3F1.
DR HPA; ENSG00000185668; Group enriched (brain, skin).
DR MIM; 602479; gene.
DR neXtProt; NX_Q03052; -.
DR OpenTargets; ENSG00000185668; -.
DR PharmGKB; PA33538; -.
DR VEuPathDB; HostDB:ENSG00000185668; -.
DR eggNOG; KOG3802; Eukaryota.
DR GeneTree; ENSGT00940000163458; -.
DR HOGENOM; CLU_013065_1_0_1; -.
DR InParanoid; Q03052; -.
DR OMA; GLAHHQW; -.
DR OrthoDB; 752252at2759; -.
DR PhylomeDB; Q03052; -.
DR TreeFam; TF316413; -.
DR PathwayCommons; Q03052; -.
DR Reactome; R-HSA-9619665; EGR2 and SOX10-mediated initiation of Schwann cell myelination.
DR SignaLink; Q03052; -.
DR SIGNOR; Q03052; -.
DR BioGRID-ORCS; 5453; 15 hits in 1090 CRISPR screens.
DR GeneWiki; POU3F1; -.
DR GenomeRNAi; 5453; -.
DR Pharos; Q03052; Tbio.
DR PRO; PR:Q03052; -.
DR Proteomes; UP000005640; Chromosome 1.
DR RNAct; Q03052; protein.
DR Bgee; ENSG00000185668; Expressed in skin of abdomen and 104 other tissues.
DR Genevisible; Q03052; HS.
DR GO; GO:0000785; C:chromatin; ISA:NTNU_SB.
DR GO; GO:0005654; C:nucleoplasm; IDA:HPA.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0005667; C:transcription regulator complex; IEA:Ensembl.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; TAS:ProtInc.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; ISA:NTNU_SB.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:MGI.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; IDA:ARUK-UCL.
DR GO; GO:0008366; P:axon ensheathment; TAS:ProtInc.
DR GO; GO:0030900; P:forebrain development; IEA:Ensembl.
DR GO; GO:0030216; P:keratinocyte differentiation; IEA:Ensembl.
DR GO; GO:0022011; P:myelination in peripheral nervous system; IEA:Ensembl.
DR GO; GO:0010628; P:positive regulation of gene expression; ISS:UniProtKB.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; ISS:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd00086; homeodomain; 1.
DR Gene3D; 1.10.260.40; -; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR InterPro; IPR013847; POU.
DR InterPro; IPR000327; POU_dom.
DR InterPro; IPR016362; TF_POU_3.
DR Pfam; PF00046; Homeodomain; 1.
DR Pfam; PF00157; Pou; 1.
DR PIRSF; PIRSF002629; Transcription_factor_POU; 1.
DR PRINTS; PR00028; POUDOMAIN.
DR SMART; SM00389; HOX; 1.
DR SMART; SM00352; POU; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR SUPFAM; SSF47413; SSF47413; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
DR PROSITE; PS00035; POU_1; 1.
DR PROSITE; PS00465; POU_2; 1.
DR PROSITE; PS51179; POU_3; 1.
PE 2: Evidence at transcript level;
KW Activator; DNA-binding; Homeobox; Nucleus; Reference proteome; Repressor;
KW Transcription; Transcription regulation.
FT CHAIN 1..451
FT /note="POU domain, class 3, transcription factor 1"
FT /id="PRO_0000100720"
FT DOMAIN 247..321
FT /note="POU-specific"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00530"
FT DNA_BIND 339..398
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 1..21
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 69..114
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 127..154
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 186..253
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 395..451
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 437..451
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 27..28
FT /note="Missing (in Ref. 1; AAA59965)"
FT /evidence="ECO:0000305"
FT CONFLICT 87..88
FT /note="GP -> A (in Ref. 1; AAA59965 and 4; CAA79158)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 451 AA; 45496 MW; 563217BD6A3D00CD CRC64;
MATTAQYLPR GPGGGAGGTG PLMHPDAAAA AAAAAAAERL HAGAAYREVQ KLMHHEWLGA
GAGHPVGLAH PQWLPTGGGG GGDWAGGPHL EHGKAGGGGT GRADDGGGGG GFHARLVHQG
AAHAGAAWAQ GSTAHHLGPA MSPSPGASGG HQPQPLGLYA QAAYPGGGGG GLAGMLAAGG
GGAGPGLHHA LHEDGHEAQL EPSPPPHLGA HGHAHGHAHA GGLHAAAAHL HPGAGGGGSS
VGEHSDEDAP SSDDLEQFAK QFKQRRIKLG FTQADVGLAL GTLYGNVFSQ TTICRFEALQ
LSFKNMCKLK PLLNKWLEET DSSSGSPTNL DKIAAQGRKR KKRTSIEVGV KGALESHFLK
CPKPSAHEIT GLADSLQLEK EVVRVWFCNR RQKEKRMTPA AGAGHPPMDD VYAPGELGPG
GGGASPPSAP PPPPPAALHH HHHHTLPGSV Q
