PO3F1_MOUSE
ID PO3F1_MOUSE Reviewed; 449 AA.
AC P21952;
DT 01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1991, sequence version 1.
DT 03-AUG-2022, entry version 177.
DE RecName: Full=POU domain, class 3, transcription factor 1;
DE AltName: Full=Octamer-binding protein 6;
DE Short=Oct-6;
DE AltName: Full=Octamer-binding transcription factor 6;
DE Short=OTF-6;
DE AltName: Full=POU domain transcription factor SCIP;
GN Name=Pou3f1; Synonyms=Oct6, Otf-6, Otf6, Scip;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, AND
RP DEVELOPMENTAL STAGE.
RC STRAIN=C57BL/6 X CBA; TISSUE=Brain;
RX PubMed=1979677; DOI=10.1093/nar/18.24.7357;
RA Meijer D., Graus A., Kraay R., Langeveld A., Mulder M.P., Grosveld G.;
RT "The octamer binding factor Oct6: cDNA cloning and expression in early
RT embryonic cells.";
RL Nucleic Acids Res. 18:7357-7365(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RC TISSUE=Brain;
RX PubMed=1976514; DOI=10.1002/j.1460-2075.1990.tb07585.x;
RA Suzuki N., Rohdewohld H., Neuman T., Gruss P., Schoeler H.R.;
RT "Oct-6: a POU transcription factor expressed in embryonal stem cells and in
RT the developing brain.";
RL EMBO J. 9:3723-3732(1990).
RN [3]
RP NUCLEOTIDE SEQUENCE.
RX PubMed=1840678; DOI=10.1093/nar/19.4.956;
RA Zimmerman E.C., Jones C.M., Fet V., Hogan B.L.M., Magnuson M.A.;
RT "Nucleotide sequence of mouse SCIP cDNA, a POU-domain transcription
RT factor.";
RL Nucleic Acids Res. 19:956-956(1991).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1565620; DOI=10.1073/pnas.89.8.3280;
RA Hara Y., Rovescalli C., Kim Y., Nirenberg M.;
RT "Structure and evolution of four POU domain genes expressed in mouse
RT brain.";
RL Proc. Natl. Acad. Sci. U.S.A. 89:3280-3284(1992).
RN [5]
RP DEVELOPMENTAL STAGE.
RX PubMed=10068633; DOI=10.1242/dev.126.7.1397;
RA Zorick T.S., Syroid D.E., Brown A., Gridley T., Lemke G.;
RT "Krox-20 controls SCIP expression, cell cycle exit and susceptibility to
RT apoptosis in developing myelinating Schwann cells.";
RL Development 126:1397-1406(1999).
RN [6]
RP FUNCTION.
RX PubMed=18505825; DOI=10.1128/mcb.00338-08;
RA Hoser M., Potzner M.R., Koch J.M., Boesl M.R., Wegner M., Sock E.;
RT "Sox12 deletion in the mouse reveals nonreciprocal redundancy with the
RT related Sox4 and Sox11 transcription factors.";
RL Mol. Cell. Biol. 28:4675-4687(2008).
CC -!- FUNCTION: Transcription factor that binds to the octamer motif (5'-
CC ATTTGCAT-3') (PubMed:1979677, PubMed:1976514). Acts as a
CC transcriptional activator when binding cooperatively with SOX4, SOX11,
CC or SOX12 to gene promoters (PubMed:18505825). Acts as a transcriptional
CC repressor of myelin-specific genes (By similarity).
CC {ECO:0000250|UniProtKB:P20267, ECO:0000269|PubMed:18505825,
CC ECO:0000269|PubMed:1976514, ECO:0000269|PubMed:1979677}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:1979677}.
CC -!- DEVELOPMENTAL STAGE: Expressed in embryonal stem cells and in the
CC developing brain (PubMed:1979677). Down-regulated in embryonic stem
CC cells upon differentiation (PubMed:1979677). Expressed in the sciatic
CC nerves at postnatal days P6 to P12 (PubMed:10068633).
CC {ECO:0000269|PubMed:10068633, ECO:0000269|PubMed:1979677}.
CC -!- SIMILARITY: Belongs to the POU transcription factor family. Class-3
CC subfamily. {ECO:0000305}.
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DR EMBL; X54628; CAA38445.1; -; mRNA.
DR EMBL; X56959; CAA40280.1; -; mRNA.
DR EMBL; X57482; CAA40720.1; -; mRNA.
DR EMBL; M88302; AAA39963.1; -; Genomic_DNA.
DR CCDS; CCDS57296.1; -.
DR PIR; S30205; S30205.
DR RefSeq; NP_035271.1; NM_011141.2.
DR PDB; 2XSD; X-ray; 2.05 A; C=240-402.
DR PDBsum; 2XSD; -.
DR AlphaFoldDB; P21952; -.
DR SMR; P21952; -.
DR BioGRID; 202305; 1.
DR STRING; 10090.ENSMUSP00000137374; -.
DR PhosphoSitePlus; P21952; -.
DR MaxQB; P21952; -.
DR PaxDb; P21952; -.
DR PeptideAtlas; P21952; -.
DR PRIDE; P21952; -.
DR ProteomicsDB; 289354; -.
DR Antibodypedia; 17662; 273 antibodies from 33 providers.
DR DNASU; 18991; -.
DR Ensembl; ENSMUST00000053491; ENSMUSP00000137374; ENSMUSG00000090125.
DR GeneID; 18991; -.
DR KEGG; mmu:18991; -.
DR UCSC; uc029uzh.1; mouse.
DR CTD; 5453; -.
DR MGI; MGI:101896; Pou3f1.
DR VEuPathDB; HostDB:ENSMUSG00000090125; -.
DR eggNOG; KOG3802; Eukaryota.
DR GeneTree; ENSGT00940000163458; -.
DR HOGENOM; CLU_013065_1_0_1; -.
DR InParanoid; P21952; -.
DR OMA; GLAHHQW; -.
DR OrthoDB; 752252at2759; -.
DR PhylomeDB; P21952; -.
DR TreeFam; TF316413; -.
DR BioGRID-ORCS; 18991; 2 hits in 74 CRISPR screens.
DR ChiTaRS; Pou3f1; mouse.
DR PRO; PR:P21952; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; P21952; protein.
DR Bgee; ENSMUSG00000090125; Expressed in CA1 field of hippocampus and 162 other tissues.
DR Genevisible; P21952; MM.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0005667; C:transcription regulator complex; IDA:MGI.
DR GO; GO:0003677; F:DNA binding; IDA:MGI.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISO:MGI.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; ISO:MGI.
DR GO; GO:0001227; F:DNA-binding transcription repressor activity, RNA polymerase II-specific; ISO:MGI.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; ISO:MGI.
DR GO; GO:0043565; F:sequence-specific DNA binding; ISO:MGI.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:MGI.
DR GO; GO:0008544; P:epidermis development; IGI:MGI.
DR GO; GO:0030900; P:forebrain development; IMP:MGI.
DR GO; GO:0010001; P:glial cell differentiation; ISO:MGI.
DR GO; GO:0030216; P:keratinocyte differentiation; IGI:MGI.
DR GO; GO:0042552; P:myelination; IMP:MGI.
DR GO; GO:0022011; P:myelination in peripheral nervous system; IGI:MGI.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; ISO:MGI.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; ISO:MGI.
DR GO; GO:0010628; P:positive regulation of gene expression; IDA:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISO:MGI.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; ISO:MGI.
DR GO; GO:0014044; P:Schwann cell development; IMP:MGI.
DR CDD; cd00086; homeodomain; 1.
DR DisProt; DP02410; -.
DR Gene3D; 1.10.260.40; -; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR InterPro; IPR013847; POU.
DR InterPro; IPR000327; POU_dom.
DR InterPro; IPR016362; TF_POU_3.
DR Pfam; PF00046; Homeodomain; 1.
DR Pfam; PF00157; Pou; 1.
DR PIRSF; PIRSF002629; Transcription_factor_POU; 1.
DR PRINTS; PR00028; POUDOMAIN.
DR SMART; SM00389; HOX; 1.
DR SMART; SM00352; POU; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR SUPFAM; SSF47413; SSF47413; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
DR PROSITE; PS00035; POU_1; 1.
DR PROSITE; PS00465; POU_2; 1.
DR PROSITE; PS51179; POU_3; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Activator; DNA-binding; Homeobox; Nucleus;
KW Reference proteome; Repressor; Transcription; Transcription regulation.
FT CHAIN 1..449
FT /note="POU domain, class 3, transcription factor 1"
FT /id="PRO_0000100721"
FT DOMAIN 245..319
FT /note="POU-specific"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00530"
FT DNA_BIND 337..396
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 1..22
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 76..108
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 132..152
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 184..251
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 393..449
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 435..449
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 35
FT /note="Missing (in Ref. 2; CAA40720)"
FT /evidence="ECO:0000305"
FT HELIX 250..266
FT /evidence="ECO:0007829|PDB:2XSD"
FT HELIX 271..282
FT /evidence="ECO:0007829|PDB:2XSD"
FT HELIX 288..295
FT /evidence="ECO:0007829|PDB:2XSD"
FT HELIX 301..318
FT /evidence="ECO:0007829|PDB:2XSD"
FT HELIX 346..358
FT /evidence="ECO:0007829|PDB:2XSD"
FT HELIX 364..374
FT /evidence="ECO:0007829|PDB:2XSD"
FT HELIX 378..392
FT /evidence="ECO:0007829|PDB:2XSD"
SQ SEQUENCE 449 AA; 45324 MW; 82DD1335A0C4F43C CRC64;
MATTAQYLPR GPGGGAGGTG PLMHPDAAAA AAAAAERLHA GAAYREVQKL MHHEWLGAGA
GHPVGLAHPQ WLPTGGGGGG DWAGGPHLEH GKAGGGGTGR ADDGGGGGGF HARLVHQGAA
HAGAAWAQGG TAHHLGPAMS PSPGAGGGHQ PQPLGLYAQA AYPGGGGGGL AGMLAAGGGG
AGPGLHHALH EDGHEAQLEP SPPPHLGAHG HAHGHAHAGG LHAAAAHLHP GAGGGGSSVG
EHSDEDAPSS DDLEQFAKQF KQRRIKLGFT QADVGLALGT LYGNVFSQTT ICRFEALQLS
FKNMCKLKPL LNKWLEETDS SSGSPTNLDK IAAQGRKRKK RTSIEVGVKG ALESHFLKCP
KPSAHEITGL ADSLQLEKEV VRVWFCNRRQ KEKRMTPAAG AGHPPMDDVY APGELGPGGG
SASPPSAPPP PPPAALHHHH HHTLPGSVQ
