PO3F3_MOUSE
ID PO3F3_MOUSE Reviewed; 497 AA.
AC P31361;
DT 01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 2.
DT 03-AUG-2022, entry version 168.
DE RecName: Full=POU domain, class 3, transcription factor 3;
DE AltName: Full=Brain-specific homeobox/POU domain protein 1;
DE Short=Brain-1;
DE Short=Brn-1;
DE AltName: Full=Octamer-binding protein 8;
DE Short=Oct-8;
DE AltName: Full=Octamer-binding transcription factor 8;
DE Short=OTF-8;
GN Name=Pou3f3; Synonyms=Brn-1, Brn1, Otf8;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1565620; DOI=10.1073/pnas.89.8.3280;
RA Hara Y., Rovescalli C., Kim Y., Nirenberg M.;
RT "Structure and evolution of four POU domain genes expressed in mouse
RT brain.";
RL Proc. Natl. Acad. Sci. U.S.A. 89:3280-3284(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=10473120; DOI=10.1016/s0925-4773(99)00067-2;
RA Mihailescu D., Kuery P., Monard D.;
RT "An octamer-binding site is crucial for the activity of an enhancer active
RT at the embryonic met-/mesencephalic junction.";
RL Mech. Dev. 84:55-67(1999).
RN [4]
RP FUNCTION, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=12130536; DOI=10.1101/gad.978002;
RA Sugitani Y., Nakai S., Minowa O., Nishi M., Jishage K., Kawano H., Mori K.,
RA Ogawa M., Noda T.;
RT "Brn-1 and Brn-2 share crucial roles in the production and positioning of
RT mouse neocortical neurons.";
RL Genes Dev. 16:1760-1765(2002).
RN [5]
RP DISRUPTION PHENOTYPE.
RX PubMed=11859196; DOI=10.1126/science.1067132;
RA McEvilly R.J., de Diaz M.O., Schonemann M.D., Hooshmand F., Rosenfeld M.G.;
RT "Transcriptional regulation of cortical neuron migration by POU domain
RT factors.";
RL Science 295:1528-1532(2002).
RN [6]
RP FUNCTION.
RX PubMed=19527706; DOI=10.1016/j.ydbio.2009.06.011;
RA Wolf M., Lommes P., Sock E., Reiprich S., Friedrich R.P., Kriesch J.,
RA Stolt C.C., Bermingham J.R. Jr., Wegner M.;
RT "Replacement of related POU transcription factors leads to severe defects
RT in mouse forebrain development.";
RL Dev. Biol. 332:418-428(2009).
CC -!- FUNCTION: Transcription factor that acts synergistically with SOX11 and
CC SOX4 (By similarity). Plays a role in neuronal development. Is
CC implicated in an enhancer activity at the embryonic met-mesencephalic
CC junction; the enhancer element contains the octamer motif (5'-ATTTGCAT-
CC 3'). {ECO:0000250|UniProtKB:Q63262, ECO:0000269|PubMed:10473120,
CC ECO:0000269|PubMed:12130536, ECO:0000269|PubMed:19527706}.
CC -!- SUBUNIT: Homodimer. {ECO:0000250|UniProtKB:P20264}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108,
CC ECO:0000255|PROSITE-ProRule:PRU00530, ECO:0000269|PubMed:10473120}.
CC -!- TISSUE SPECIFICITY: Brain.
CC -!- DEVELOPMENTAL STAGE: Expressed in the developing neocortex, both in the
CC late precursor cells and in the migrating neurons.
CC {ECO:0000269|PubMed:12130536}.
CC -!- DISRUPTION PHENOTYPE: Neonatal death at least 36 hours postpartum. Loss
CC of both Pou3f3 and Pou3f2 leads to abnormal formation of the neocortex
CC with dramatically reduced production of layer IV-II neurons and
CC defective migration of neurons. {ECO:0000269|PubMed:11859196,
CC ECO:0000269|PubMed:12130536}.
CC -!- MISCELLANEOUS: Can fully replace Pou3f1 in Schwann cells of embryonic
CC mice and rescue peripheral nervous system development in these animals,
CC although severe defects in forebrain development are still observed.
CC -!- SIMILARITY: Belongs to the POU transcription factor family. Class-3
CC subfamily. {ECO:0000305}.
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DR EMBL; M88299; AAA39960.1; -; Genomic_DNA.
DR EMBL; AC122898; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS14917.1; -.
DR PIR; S31223; S31223.
DR RefSeq; NP_032926.2; NM_008900.2.
DR AlphaFoldDB; P31361; -.
DR SMR; P31361; -.
DR BioGRID; 202307; 6.
DR STRING; 10090.ENSMUSP00000052088; -.
DR iPTMnet; P31361; -.
DR PhosphoSitePlus; P31361; -.
DR MaxQB; P31361; -.
DR PaxDb; P31361; -.
DR PeptideAtlas; P31361; -.
DR PRIDE; P31361; -.
DR ProteomicsDB; 289355; -.
DR Antibodypedia; 32979; 161 antibodies from 33 providers.
DR DNASU; 18993; -.
DR Ensembl; ENSMUST00000054883; ENSMUSP00000052088; ENSMUSG00000045515.
DR Ensembl; ENSMUST00000239323; ENSMUSP00000159226; ENSMUSG00000045515.
DR GeneID; 18993; -.
DR KEGG; mmu:18993; -.
DR UCSC; uc007auw.1; mouse.
DR CTD; 5455; -.
DR MGI; MGI:102564; Pou3f3.
DR VEuPathDB; HostDB:ENSMUSG00000045515; -.
DR eggNOG; KOG3802; Eukaryota.
DR GeneTree; ENSGT00940000163665; -.
DR HOGENOM; CLU_013065_1_2_1; -.
DR InParanoid; P31361; -.
DR OMA; CKRMFSE; -.
DR OrthoDB; 752252at2759; -.
DR PhylomeDB; P31361; -.
DR TreeFam; TF316413; -.
DR BioGRID-ORCS; 18993; 3 hits in 72 CRISPR screens.
DR ChiTaRS; Pou3f3; mouse.
DR PRO; PR:P31361; -.
DR Proteomes; UP000000589; Chromosome 1.
DR RNAct; P31361; protein.
DR Bgee; ENSMUSG00000045515; Expressed in medial ganglionic eminence and 198 other tissues.
DR Genevisible; P31361; MM.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; IDA:MGI.
DR GO; GO:0003677; F:DNA binding; IDA:MGI.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISO:MGI.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; ISO:MGI.
DR GO; GO:0071837; F:HMG box domain binding; IPI:UniProtKB.
DR GO; GO:0042803; F:protein homodimerization activity; ISS:UniProtKB.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:UniProtKB.
DR GO; GO:0021799; P:cerebral cortex radially oriented cell migration; IGI:MGI.
DR GO; GO:0048878; P:chemical homeostasis; IMP:MGI.
DR GO; GO:0021869; P:forebrain ventricular zone progenitor cell division; IGI:MGI.
DR GO; GO:0001822; P:kidney development; IMP:MGI.
DR GO; GO:0072218; P:metanephric ascending thin limb development; IMP:UniProtKB.
DR GO; GO:0072240; P:metanephric DCT cell differentiation; IMP:UniProtKB.
DR GO; GO:0072236; P:metanephric loop of Henle development; IMP:UniProtKB.
DR GO; GO:0072227; P:metanephric macula densa development; IMP:UniProtKB.
DR GO; GO:0072233; P:metanephric thick ascending limb development; IMP:UniProtKB.
DR GO; GO:0043066; P:negative regulation of apoptotic process; IMP:UniProtKB.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; ISO:MGI.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IDA:UniProtKB.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; IMP:UniProtKB.
DR GO; GO:0010628; P:positive regulation of gene expression; ISO:MGI.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IGI:MGI.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IMP:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0071918; P:urea transmembrane transport; IMP:MGI.
DR CDD; cd00086; homeodomain; 1.
DR Gene3D; 1.10.260.40; -; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR InterPro; IPR013847; POU.
DR InterPro; IPR000327; POU_dom.
DR InterPro; IPR016362; TF_POU_3.
DR Pfam; PF00046; Homeodomain; 1.
DR Pfam; PF00157; Pou; 1.
DR PIRSF; PIRSF002629; Transcription_factor_POU; 1.
DR PRINTS; PR00028; POUDOMAIN.
DR SMART; SM00389; HOX; 1.
DR SMART; SM00352; POU; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR SUPFAM; SSF47413; SSF47413; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
DR PROSITE; PS00035; POU_1; 1.
DR PROSITE; PS00465; POU_2; 1.
DR PROSITE; PS51179; POU_3; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; DNA-binding; Homeobox; Neurogenesis; Nucleus;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..497
FT /note="POU domain, class 3, transcription factor 3"
FT /id="PRO_0000100728"
FT DOMAIN 311..385
FT /note="POU-specific"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00530"
FT DNA_BIND 403..462
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 32..62
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 121..189
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 230..316
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 458..497
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 132..147
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 168..182
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 270..287
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 462..497
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 10
FT /note="L -> Q (in Ref. 1; AAA39960)"
FT /evidence="ECO:0000305"
FT CONFLICT 81..82
FT /note="Missing (in Ref. 1; AAA39960)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 497 AA; 50200 MW; 4B1860BEED53F8B0 CRC64;
MATAASNPYL PGNSLLTAGS IVHSDAAGAG GGGGGGGGGG GGAGGGGGGM QPGSAAVTSG
AYRGDPSSVK MVQSDFMQGA MAASNGGHML SHAHQWVTAL PHAAAAAAAA AAAAVEASSP
WSGSAVGMAG SPQQPPQPPP PPPQGPDVKG GAGREDLHAG TALHHRGPPH LGPPPPPPHQ
GHPGGWGAAA AAAAAAAAAA AAAHLPSMAG GQQPPPQSLL YSQPGGFTVN GMLSAPPGPG
GGGGGAGGGA QSLVHPGLVR GDTPELAEHH HHHHHHAHPH PPHPHHAQGP PHHGGGGAGP
GLNSHDPHSD EDTPTSDDLE QFAKQFKQRR IKLGFTQADV GLALGTLYGN VFSQTTICRF
EALQLSFKNM CKLKPLLNKW LEEADSSTGS PTSIDKIAAQ GRKRKKRTSI EVSVKGALES
HFLKCPKPSA QEITNLADSL QLEKEVVRVW FCNRRQKEKR MTPPGIQQQT PDDVYSQVGT
VSADTPPPHH GLQTSVQ
