ID   PO4F3_DANRE             Reviewed;         331 AA.
AC   Q90435; Q52NV5;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   24-NOV-2009, sequence version 2.
DT   03-AUG-2022, entry version 147.
DE   RecName: Full=POU domain, class 4, transcription factor 3;
DE   AltName: Full=Brain-specific homeobox/POU domain protein 3.1;
DE            Short=Brain-3.1;
DE            Short=zfBrn-3.1;
GN   Name=pou4f3; Synonyms=brn-3.1, brn-3c, brn3.1, brn3c;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RX   PubMed=15930106; DOI=10.1242/dev.01861;
RA   Xiao T., Roeser T., Staub W., Baier H.;
RT   "A GFP-based genetic screen reveals mutations that disrupt the architecture
RT   of the zebrafish retinotectal projection.";
RL   Development 132:2955-2967(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 196-311, TISSUE SPECIFICITY, AND
RP   DEVELOPMENTAL STAGE.
RX   PubMed=8605028; DOI=10.1006/bbrc.1996.0274;
RA   Sampath K., Stuart G.W.;
RT   "Developmental expression of class III and IV POU domain genes in the
RT   zebrafish.";
RL   Biochem. Biophys. Res. Commun. 219:565-571(1996).
RN   [3]
RP   TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX   PubMed=15042710; DOI=10.1002/dvdy.10475;
RA   DeCarvalho A.C., Cappendijk S.L., Fadool J.M.;
RT   "Developmental expression of the POU domain transcription factor Brn-3b
RT   (Pou4f2) in the lateral line and visual system of zebrafish.";
RL   Dev. Dyn. 229:869-876(2004).
CC   -!- FUNCTION: Acts as a transcriptional activator. Acts by binding to
CC       sequences related to the consensus octamer motif 5'-ATGCAAAT-3' in the
CC       regulatory regions of its target genes. May play a role in specifying
CC       terminally differentiated neuronal phenotypes.
CC       {ECO:0000250|UniProtKB:Q63955}.
CC   -!- SUBUNIT: Interaction with ISL1. {ECO:0000250|UniProtKB:Q63955}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q15319}. Cytoplasm
CC       {ECO:0000250|UniProtKB:Q15319}. Note=Preferentially localized in the
CC       nucleus. {ECO:0000250|UniProtKB:Q15319}.
CC   -!- TISSUE SPECIFICITY: Expressed in the nervous system. Expressed in the
CC       otic vesicle during embryogenesis. Expressed in the adult retina in a
CC       subset of retinal ganglion cells (RGCs), and at a lower level in the
CC       adult tectum. Not expressed in the adult olfactory bulb.
CC       {ECO:0000269|PubMed:15042710, ECO:0000269|PubMed:15930106,
CC       ECO:0000269|PubMed:8605028}.
CC   -!- DEVELOPMENTAL STAGE: Weakly expressed during embryogenesis, with
CC       stronger expression in the adult eye. {ECO:0000269|PubMed:15042710,
CC       ECO:0000269|PubMed:8605028}.
CC   -!- SIMILARITY: Belongs to the POU transcription factor family. Class-4
CC       subfamily. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AY995217; AAX86839.1; -; mRNA.
DR   EMBL; U43658; AAB00435.1; -; Genomic_DNA.
DR   RefSeq; NP_571353.1; NM_131278.1.
DR   AlphaFoldDB; Q90435; -.
DR   SMR; Q90435; -.
DR   STRING; 7955.ENSDARP00000124728; -.
DR   PaxDb; Q90435; -.
DR   PRIDE; Q90435; -.
DR   Ensembl; ENSDART00000149175; ENSDARP00000124728; ENSDARG00000006206.
DR   GeneID; 30534; -.
DR   KEGG; dre:30534; -.
DR   CTD; 5459; -.
DR   ZFIN; ZDB-GENE-990415-24; pou4f3.
DR   eggNOG; KOG1168; Eukaryota.
DR   GeneTree; ENSGT00940000160880; -.
DR   HOGENOM; CLU_013065_0_0_1; -.
DR   InParanoid; Q90435; -.
DR   OMA; MKYSAAH; -.
DR   OrthoDB; 929123at2759; -.
DR   PhylomeDB; Q90435; -.
DR   PRO; PR:Q90435; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Chromosome 9.
DR   Bgee; ENSDARG00000006206; Expressed in retina and 7 other tissues.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0043565; F:sequence-specific DNA binding; ISS:UniProtKB.
DR   GO; GO:0007420; P:brain development; IEP:ZFIN.
DR   GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR   GO; GO:0048839; P:inner ear development; IEA:InterPro.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   GO; GO:0007605; P:sensory perception of sound; IEA:InterPro.
DR   CDD; cd00086; homeodomain; 1.
DR   CDD; cd00093; HTH_XRE; 1.
DR   Gene3D; 1.10.260.40; -; 1.
DR   InterPro; IPR001387; Cro/C1-type_HTH.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR017970; Homeobox_CS.
DR   InterPro; IPR001356; Homeobox_dom.
DR   InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR   InterPro; IPR013847; POU.
DR   InterPro; IPR032961; POU4F3.
DR   InterPro; IPR000327; POU_dom.
DR   PANTHER; PTHR11636:SF43; PTHR11636:SF43; 1.
DR   Pfam; PF00046; Homeodomain; 1.
DR   Pfam; PF00157; Pou; 1.
DR   PRINTS; PR00028; POUDOMAIN.
DR   SMART; SM00389; HOX; 1.
DR   SMART; SM00352; POU; 1.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   SUPFAM; SSF47413; SSF47413; 1.
DR   PROSITE; PS00027; HOMEOBOX_1; 1.
DR   PROSITE; PS50071; HOMEOBOX_2; 1.
DR   PROSITE; PS00035; POU_1; 1.
DR   PROSITE; PS00465; POU_2; 1.
DR   PROSITE; PS51179; POU_3; 1.
PE   2: Evidence at transcript level;
KW   Activator; Cytoplasm; Differentiation; DNA-binding; Homeobox; Nucleus;
KW   Reference proteome; Transcription; Transcription regulation.
FT   CHAIN           1..331
FT                   /note="POU domain, class 4, transcription factor 3"
FT                   /id="PRO_0000100745"
FT   DOMAIN          172..249
FT                   /note="POU-specific"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00530"
FT   DNA_BIND        267..326
FT                   /note="Homeobox"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT   REGION          81..108
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        81..95
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        299
FT                   /note="A -> R (in Ref. 2; AAB00435)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   331 AA;  36550 MW;  26E3487CCCC7533A CRC64;
     MMTMNGKQHF SMHPALHPSS EGMRRVCLPA PQLQGNIFSG FDESLLARAE ALAAADIVSH
     GKSHPFKTDV TYHTMSSVPC TSSSSTVPIS HPSSNLPSHH HHHLSHQTLE GDLLDHISSS
     LSVSGMGAPP DPSVMTTQAH QHHLQMGHLH QAMAMGHPHT LSVHNGMACV NDVESDPREL
     EAFAERFKQR RIKLGVTQAD VGSALANLKI PGVGSLSQST ICRFESLTLS HNNMIALKPV
     LQAWLEEAEA AYREKNGKPD LFNGNERKRK RTSIAAPEKR SLEAYFAIQP RPSSEKIAAI
     AEKLDLKKNV VRVWFCNQRQ KQKRMKYSAV H