PO5F1_DANRE
ID PO5F1_DANRE Reviewed; 472 AA.
AC Q90270; Q90483;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 152.
DE RecName: Full=POU domain, class 5, transcription factor 1;
DE AltName: Full=POU domain protein 2;
GN Name=pou5f1; Synonyms=gp-9, pou-2, pou2;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND ALTERNATIVE SPLICING.
RC STRAIN=AB;
RX PubMed=8288127; DOI=10.1101/gad.8.1.45;
RA Takeda H., Matsuzaki T., Oki T., Miyagawa T., Amanuma H.;
RT "A novel POU domain gene, zebrafish pou2: expression and roles of two
RT alternatively spliced twin products in early development.";
RL Genes Dev. 8:45-59(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Neurula;
RX PubMed=7669688; DOI=10.1016/0925-4773(95)00360-d;
RA Hauptmann G., Gerster T.;
RT "Pou-2 -- a zebrafish gene active during cleavage stages and in the early
RT hindbrain.";
RL Mech. Dev. 51:127-138(1995).
CC -!- FUNCTION: Involved in early development of embryos, especially in the
CC process of gastrulation. May play an important role in establishing and
CC specifying rhombomeric segments. Seems to be required to maintain the
CC cells in a highly undifferentiated state. In contrast to POU2, T-POU2
CC lacks DNA-binding activity because of its incomplete pou domain
CC structure. Overexpression of POU2 does not have any effect on
CC development, whereas overexpression of t-POU2 causes developmental
CC retardation or arrest before gastrulation.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=POU2;
CC IsoId=Q90270-1; Sequence=Displayed;
CC Name=T-POU2;
CC IsoId=Q90270-2; Sequence=VSP_002337, VSP_002338;
CC -!- DEVELOPMENTAL STAGE: Maternally expressed. Present from the one-cell
CC stage to the gastrula stage. Present in all blastomeres until the
CC midblastula stage. The expression is restricted to the epiblast during
CC gastrulation, and to the neural plate after gastrulation. In the adult,
CC expression is limited to the ovary.
CC -!- SIMILARITY: Belongs to the POU transcription factor family. Class-7
CC subfamily. {ECO:0000305}.
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DR EMBL; D28548; BAA05901.1; -; mRNA.
DR EMBL; X84224; CAA59006.1; -; mRNA.
DR PIR; A49836; A49836.
DR PIR; B49836; B49836.
DR RefSeq; NP_571187.1; NM_131112.1.
DR AlphaFoldDB; Q90270; -.
DR SMR; Q90270; -.
DR STRING; 7955.ENSDARP00000065816; -.
DR PaxDb; Q90270; -.
DR GeneID; 30333; -.
DR KEGG; dre:30333; -.
DR CTD; 30333; -.
DR ZFIN; ZDB-GENE-980526-485; pou5f3.
DR eggNOG; KOG3802; Eukaryota.
DR InParanoid; Q90270; -.
DR OrthoDB; 668779at2759; -.
DR TreeFam; TF316413; -.
DR PRO; PR:Q90270; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Unplaced.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003682; F:chromatin binding; IDA:ZFIN.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IGI:ZFIN.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046982; F:protein heterodimerization activity; IPI:ZFIN.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:ZFIN.
DR GO; GO:0009653; P:anatomical structure morphogenesis; IMP:ZFIN.
DR GO; GO:0007420; P:brain development; IMP:ZFIN.
DR GO; GO:0035284; P:brain segmentation; IMP:ZFIN.
DR GO; GO:0043697; P:cell dedifferentiation; IMP:ZFIN.
DR GO; GO:0009953; P:dorsal/ventral pattern formation; IMP:ZFIN.
DR GO; GO:0007398; P:ectoderm development; IMP:ZFIN.
DR GO; GO:0009880; P:embryonic pattern specification; IMP:ZFIN.
DR GO; GO:0001706; P:endoderm formation; IMP:ZFIN.
DR GO; GO:0090504; P:epiboly; IMP:ZFIN.
DR GO; GO:0055113; P:epiboly involved in gastrulation with mouth forming second; IMP:ZFIN.
DR GO; GO:0031101; P:fin regeneration; IMP:ZFIN.
DR GO; GO:0030902; P:hindbrain development; IMP:ZFIN.
DR GO; GO:0007498; P:mesoderm development; IMP:ZFIN.
DR GO; GO:0030917; P:midbrain-hindbrain boundary development; IMP:ZFIN.
DR GO; GO:0016331; P:morphogenesis of embryonic epithelium; IMP:ZFIN.
DR GO; GO:0034728; P:nucleosome organization; IDA:ZFIN.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IGI:ZFIN.
DR GO; GO:0044030; P:regulation of DNA methylation; IGI:ZFIN.
DR GO; GO:0042663; P:regulation of endodermal cell fate specification; IGI:ZFIN.
DR GO; GO:0010468; P:regulation of gene expression; IMP:ZFIN.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd00086; homeodomain; 1.
DR Gene3D; 1.10.260.40; -; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR InterPro; IPR013847; POU.
DR InterPro; IPR000327; POU_dom.
DR Pfam; PF00046; Homeodomain; 1.
DR Pfam; PF00157; Pou; 1.
DR PRINTS; PR00028; POUDOMAIN.
DR SMART; SM00389; HOX; 1.
DR SMART; SM00352; POU; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR SUPFAM; SSF47413; SSF47413; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
DR PROSITE; PS00035; POU_1; 1.
DR PROSITE; PS00465; POU_2; 1.
DR PROSITE; PS51179; POU_3; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Developmental protein; DNA-binding; Homeobox;
KW Nucleus; Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..472
FT /note="POU domain, class 5, transcription factor 1"
FT /id="PRO_0000100752"
FT DOMAIN 249..323
FT /note="POU-specific"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00530"
FT DNA_BIND 343..402
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 127..154
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 187..255
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 127..145
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 215..248
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 386..399
FT /note="VVRVWFCNRRQKGK -> CVYGSATVDRRESV (in isoform T-
FT POU2)"
FT /evidence="ECO:0000305"
FT /id="VSP_002337"
FT VAR_SEQ 400..472
FT /note="Missing (in isoform T-POU2)"
FT /evidence="ECO:0000305"
FT /id="VSP_002338"
FT CONFLICT 160
FT /note="I -> V (in Ref. 2; CAA59006)"
FT /evidence="ECO:0000305"
FT CONFLICT 245
FT /note="N -> D (in Ref. 2; CAA59006)"
FT /evidence="ECO:0000305"
FT CONFLICT 462
FT /note="F -> L (in Ref. 2; CAA59006)"
FT /evidence="ECO:0000305"
FT CONFLICT 471
FT /note="N -> T (in Ref. 2; CAA59006)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 472 AA; 51505 MW; D11678834FBD031F CRC64;
MTERAQSPTA ADCRPYEVNR AMYPQAAGLD GLGGASLQFA HGMLQDPSLI FNKAHFNGIT
PATAQTFFPF SGDFKTNDLQ GGDFTQPKHW YPFAAPEFTG QVAGATAATQ PANISPPIGE
TREQIKMPSE VKTEKDVEEY GNEENKPPSQ YHLTAGTSSI PTGVNYYTPW NPNFWPGLSQ
ITAQANISQA PPTPSASSPS LSPSPPGNGF GSPGFFSGGT AQNIPSAQAQ SAPRSSGSSS
GGCSNSEEEE TLTTEDLEQF AKELKHKRIT LGFTQADVGL ALGNLYGKMF SQTTICRFEA
LQLSFKNMCK LKPLLQRWLN EAENSENPQD MYKIERVFVD TRKRKRRTSL EGTVRSALES
YFVKCPKPNT LEITHISDDL GLERDVVRVW FCNRRQKGKR LALPFDDECV EAQYYEQSPP
PPPHMGGTVL PGQGYPGPAH PGGAPALYMP SLHRPDVFKN GFHPGLVGHL NS
