PO5F2_HUMAN
ID PO5F2_HUMAN Reviewed; 328 AA.
AC Q8N7G0; Q15169; Q6MZL7; Q8N748;
DT 01-FEB-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=POU domain, class 5, transcription factor 2;
DE AltName: Full=Sperm 1 POU domain transcription factor;
DE Short=SPRM-1;
GN Name=POU5F2; Synonyms=SPRM1;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 148-328.
RC TISSUE=Uterus;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 168-254, AND TISSUE SPECIFICITY.
RC TISSUE=Skeletal muscle;
RX PubMed=7908264; DOI=10.1111/j.1432-1033.1994.tb18676.x;
RA Wey E., Lyons G.E., Schaefer B.W.;
RT "A human POU domain gene, mPOU, is expressed in developing brain and
RT specific adult tissues.";
RL Eur. J. Biochem. 220:753-762(1994).
CC -!- FUNCTION: Transcription factor that binds preferentially to the octamer
CC motif (5'-ATGTTAAT-3'). May exert a regulatory function in meiotic
CC events that are required for terminal differentiation of male germ cell
CC (By similarity). {ECO:0000250}.
CC -!- INTERACTION:
CC Q8N7G0; Q3LI66: KRTAP6-2; NbExp=3; IntAct=EBI-12849044, EBI-11962084;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108,
CC ECO:0000255|PROSITE-ProRule:PRU00530}.
CC -!- TISSUE SPECIFICITY: Expressed in skeletal and cardiac muscles, brain,
CC heart and lung. Little or no detectable expression found in pancreas,
CC kidney, liver or placenta. {ECO:0000269|PubMed:7908264}.
CC -!- SIMILARITY: Belongs to the POU transcription factor family. Class-5
CC subfamily. {ECO:0000305}.
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DR EMBL; AK098546; BAC05328.1; -; mRNA.
DR EMBL; BC029532; AAH29532.2; -; mRNA.
DR EMBL; BX641019; CAE46012.1; -; mRNA.
DR EMBL; Z21965; CAA79976.1; -; mRNA.
DR CCDS; CCDS59489.1; -.
DR RefSeq; NP_694948.1; NM_153216.1.
DR AlphaFoldDB; Q8N7G0; -.
DR SMR; Q8N7G0; -.
DR BioGRID; 126388; 1.
DR IntAct; Q8N7G0; 1.
DR STRING; 9606.ENSP00000464890; -.
DR iPTMnet; Q8N7G0; -.
DR PhosphoSitePlus; Q8N7G0; -.
DR BioMuta; POU5F2; -.
DR DMDM; 28201897; -.
DR EPD; Q8N7G0; -.
DR MassIVE; Q8N7G0; -.
DR PaxDb; Q8N7G0; -.
DR PeptideAtlas; Q8N7G0; -.
DR PRIDE; Q8N7G0; -.
DR Antibodypedia; 64781; 130 antibodies from 21 providers.
DR DNASU; 134187; -.
DR Ensembl; ENST00000606183.4; ENSP00000489796.1; ENSG00000248483.7.
DR GeneID; 134187; -.
DR KEGG; hsa:134187; -.
DR MANE-Select; ENST00000606183.4; ENSP00000489796.1; NM_153216.2; NP_694948.1.
DR UCSC; uc003kkl.2; human.
DR CTD; 134187; -.
DR DisGeNET; 134187; -.
DR GeneCards; POU5F2; -.
DR HGNC; HGNC:26367; POU5F2.
DR HPA; ENSG00000248483; Tissue enhanced (brain, testis).
DR neXtProt; NX_Q8N7G0; -.
DR OpenTargets; ENSG00000248483; -.
DR PharmGKB; PA162399970; -.
DR VEuPathDB; HostDB:ENSG00000248483; -.
DR eggNOG; KOG3802; Eukaryota.
DR GeneTree; ENSGT00940000164293; -.
DR HOGENOM; CLU_066243_0_0_1; -.
DR InParanoid; Q8N7G0; -.
DR OMA; GPYIALR; -.
DR OrthoDB; 1036194at2759; -.
DR PhylomeDB; Q8N7G0; -.
DR PathwayCommons; Q8N7G0; -.
DR BioGRID-ORCS; 134187; 15 hits in 1068 CRISPR screens.
DR GenomeRNAi; 134187; -.
DR Pharos; Q8N7G0; Tdark.
DR PRO; PR:Q8N7G0; -.
DR Proteomes; UP000005640; Chromosome 5.
DR RNAct; Q8N7G0; protein.
DR Bgee; ENSG00000248483; Expressed in bone marrow cell and 15 other tissues.
DR Genevisible; Q8N7G0; HS.
DR GO; GO:0000785; C:chromatin; ISA:NTNU_SB.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; ISA:NTNU_SB.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd00086; homeodomain; 1.
DR Gene3D; 1.10.260.40; -; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR InterPro; IPR013847; POU.
DR InterPro; IPR000327; POU_dom.
DR InterPro; IPR015585; POU_dom_5.
DR PANTHER; PTHR11636:SF14; PTHR11636:SF14; 1.
DR Pfam; PF00046; Homeodomain; 1.
DR Pfam; PF00157; Pou; 1.
DR PRINTS; PR00028; POUDOMAIN.
DR SMART; SM00389; HOX; 1.
DR SMART; SM00352; POU; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR SUPFAM; SSF47413; SSF47413; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
DR PROSITE; PS00035; POU_1; 1.
DR PROSITE; PS00465; POU_2; 1.
DR PROSITE; PS51179; POU_3; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Homeobox; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..328
FT /note="POU domain, class 5, transcription factor 2"
FT /id="PRO_0000100756"
FT DOMAIN 118..192
FT /note="POU-specific"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00530"
FT DNA_BIND 210..269
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 1..25
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 2
FT /note="A -> D (in Ref. 2; AAH29532)"
FT /evidence="ECO:0000305"
FT CONFLICT 229
FT /note="F -> L (in Ref. 3; CAE46012)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 328 AA; 36051 MW; DD90D7153E69A3ED CRC64;
MAGHRPSNHF CPLPGSGGGG PRGPMPLRVD TLTWLSTQAA PGRVMVWPAV RPGICPGPDV
WRIPLGPLPH EFRGWIAPCR PRLGASEAGD WLRRPSEGAL PGPYIALRSI PKLPPPEDIS
GILKELQQLA KELRQKRLSL GYSQADVGIA VGALFGKVLS QTTICRFEAQ QLSVANMWKL
RPLLKKWLKE VEAENLLGLC KMEMILQQSG KWRRASRERR IGNSLEKFFQ RCPKPTPQQI
SHIAGCLQLQ KDVVRVWFYN RSKMGSRPTN DASPREIVGT AGPPCPGAPV CFHLGLGLPV
DIPHYTRLYS AGVAHSSAPA TTLGLLRF
