PO5F2_MOUSE
ID PO5F2_MOUSE Reviewed; 329 AA.
AC Q9DAC9; Q8BVS2;
DT 01-FEB-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 25-MAY-2022, entry version 130.
DE RecName: Full=POU domain, class 5, transcription factor 2;
DE AltName: Full=Sperm 1 POU domain transcription factor;
DE Short=SPRM-1;
GN Name=Pou5f2; Synonyms=Sprm1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=7908264; DOI=10.1111/j.1432-1033.1994.tb18676.x;
RA Wey E., Lyons G.E., Schaefer B.W.;
RT "A human POU domain gene, mPOU, is expressed in developing brain and
RT specific adult tissues.";
RL Eur. J. Biochem. 220:753-762(1994).
CC -!- FUNCTION: Transcription factor that binds preferentially to the octamer
CC motif (5'-ATGTTAAT-3'). May exert a regulatory function in meiotic
CC events that are required for terminal differentiation of male germ cell
CC (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108,
CC ECO:0000255|PROSITE-ProRule:PRU00530}.
CC -!- TISSUE SPECIFICITY: In adult brain, expressed in the olfactory bulb,
CC becoming specifically concentrated in the mitral cell layer. Also found
CC in the pyramidal cell layer of the hippocampus, in the granule cell
CC layer of the cerebellum and in the cortex.
CC {ECO:0000269|PubMed:7908264}.
CC -!- DEVELOPMENTAL STAGE: Low expression levels observed at 12 dpc in neural
CC tube and brain. On days 15.5 and 16.5, expression levels increased and
CC became localized specifically to the forebrain, with highest levels
CC found in the laminar region of the forebrain cortex. Also expressed in
CC the mitral region of the olfactory bulb. {ECO:0000269|PubMed:7908264}.
CC -!- SIMILARITY: Belongs to the POU transcription factor family. Class-5
CC subfamily. {ECO:0000305}.
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DR EMBL; AK005948; BAB24332.1; -; mRNA.
DR EMBL; AK076753; BAC36466.1; -; mRNA.
DR CCDS; CCDS56895.1; -.
DR RefSeq; NP_083591.1; NM_029315.1.
DR AlphaFoldDB; Q9DAC9; -.
DR SMR; Q9DAC9; -.
DR STRING; 10090.ENSMUSP00000135597; -.
DR PhosphoSitePlus; Q9DAC9; -.
DR MaxQB; Q9DAC9; -.
DR PaxDb; Q9DAC9; -.
DR PRIDE; Q9DAC9; -.
DR ProteomicsDB; 289650; -.
DR GeneID; 75507; -.
DR KEGG; mmu:75507; -.
DR UCSC; uc011zcd.1; mouse.
DR CTD; 134187; -.
DR MGI; MGI:1922757; Pou5f2.
DR eggNOG; KOG3802; Eukaryota.
DR InParanoid; Q9DAC9; -.
DR OrthoDB; 841019at2759; -.
DR PhylomeDB; Q9DAC9; -.
DR TreeFam; TF316413; -.
DR BioGRID-ORCS; 75507; 0 hits in 70 CRISPR screens.
DR PRO; PR:Q9DAC9; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; Q9DAC9; protein.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:MGI.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd00086; homeodomain; 1.
DR Gene3D; 1.10.260.40; -; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR InterPro; IPR013847; POU.
DR InterPro; IPR000327; POU_dom.
DR InterPro; IPR015585; POU_dom_5.
DR PANTHER; PTHR11636:SF14; PTHR11636:SF14; 1.
DR Pfam; PF00046; Homeodomain; 1.
DR Pfam; PF00157; Pou; 1.
DR PRINTS; PR00028; POUDOMAIN.
DR SMART; SM00389; HOX; 1.
DR SMART; SM00352; POU; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR SUPFAM; SSF47413; SSF47413; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
DR PROSITE; PS00035; POU_1; 1.
DR PROSITE; PS00465; POU_2; 1.
DR PROSITE; PS51179; POU_3; 1.
PE 2: Evidence at transcript level;
KW DNA-binding; Homeobox; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..329
FT /note="POU domain, class 5, transcription factor 2"
FT /id="PRO_0000100757"
FT DOMAIN 107..181
FT /note="POU-specific"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00530"
FT DNA_BIND 199..258
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 1..24
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 292
FT /note="T -> P (in Ref. 1; BAC36466)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 329 AA; 36411 MW; A882639979987F31 CRC64;
MAGRRSSNVF PLSGNSGGGL EVDTPTWLSS QAATSRLMVR PSMGPGICPG PEVWGVPLGS
SPYEFRGGIA PYRACEARAW SQSSSEDTCP GPYIALRYMP NLALPEDVSA IQKEMEQLAK
ELRQKRMTLG YSQADVGFAV GAMFGKVLSQ TTICRFEAQQ LSLANMWKLR PLLKMWLEEV
DEKNLLGICR MEMILEQARK RRRASRERRI GSNLEKLFLQ CPEPTPQQIS YIAGRLRLQK
DLVQVWFSNR SQMGSWPTND TSRREDVGAT GSPFPGPPVC FPMAPGLHFD FTHYEGSCLT
PLYSSTPFPV RGALLSAPTT TLGLPRLSS
