PO6F1_HUMAN
ID PO6F1_HUMAN Reviewed; 301 AA.
AC Q14863; Q15944; Q6DK47; Q7Z7P6;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 190.
DE RecName: Full=POU domain, class 6, transcription factor 1;
DE AltName: Full=Brain-specific homeobox/POU domain protein 5;
DE Short=Brain-5;
DE Short=Brn-5;
DE AltName: Full=mPOU homeobox protein;
GN Name=POU6F1; Synonyms=BRN5, MPOU, TCFB1;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Skeletal muscle;
RX PubMed=7908264; DOI=10.1111/j.1432-1033.1994.tb18676.x;
RA Wey E., Lyons G.E., Schaefer B.W.;
RT "A human POU domain gene, mPOU, is expressed in developing brain and
RT specific adult tissues.";
RL Eur. J. Biochem. 220:753-762(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=8102789; DOI=10.1128/mcb.13.9.5450-5460.1993;
RA Messier H., Brickner H., Gaikwad J., Fotedar A.;
RT "A novel POU domain protein which binds to the T-cell receptor beta
RT enhancer.";
RL Mol. Cell. Biol. 13:5450-5460(1993).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain, and Salivary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Transcription factor that binds preferentially to a variant
CC of the octamer motif (5'-ATGATAAT-3'). {ECO:0000250}.
CC -!- INTERACTION:
CC Q14863; O00154-4: ACOT7; NbExp=3; IntAct=EBI-18138148, EBI-12007918;
CC Q14863; Q13952-2: NFYC; NbExp=3; IntAct=EBI-18138148, EBI-11956831;
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- TISSUE SPECIFICITY: In the embryo, expressed exclusively in the
CC developing brain, whereas in the adult its expression is restricted to
CC brain, heart, skeletal muscle and lung. In the brain, the highest
CC expression levels are found in specific cell layers of the cortex, the
CC olfactory bulb, the hippocampus and the cerebellum.
CC -!- SIMILARITY: Belongs to the POU transcription factor family. Class-6
CC subfamily. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; Z21966; CAA79977.1; -; mRNA.
DR EMBL; Z21967; CAA79978.1; -; mRNA.
DR EMBL; L14482; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; CH471111; EAW58180.1; -; Genomic_DNA.
DR EMBL; BC051326; AAH51326.2; -; mRNA.
DR EMBL; BC074765; AAH74765.3; -; mRNA.
DR PIR; A54687; A54687.
DR RefSeq; NP_001317351.1; NM_001330422.1.
DR RefSeq; NP_002693.3; NM_002702.3.
DR RefSeq; XP_016875013.1; XM_017019524.1.
DR RefSeq; XP_016875014.1; XM_017019525.1.
DR RefSeq; XP_016875015.1; XM_017019526.1.
DR RefSeq; XP_016875016.1; XM_017019527.1.
DR RefSeq; XP_016875017.1; XM_017019528.1.
DR RefSeq; XP_016875018.1; XM_017019529.1.
DR RefSeq; XP_016875019.1; XM_017019530.1.
DR PDB; 3D1N; X-ray; 2.51 A; I/J/K/L/M/N/O/P=142-292.
DR PDBsum; 3D1N; -.
DR AlphaFoldDB; Q14863; -.
DR SMR; Q14863; -.
DR BioGRID; 111459; 3.
DR IntAct; Q14863; 3.
DR STRING; 9606.ENSP00000373895; -.
DR iPTMnet; Q14863; -.
DR PhosphoSitePlus; Q14863; -.
DR BioMuta; POU6F1; -.
DR DMDM; 2495306; -.
DR MassIVE; Q14863; -.
DR PaxDb; Q14863; -.
DR PeptideAtlas; Q14863; -.
DR PRIDE; Q14863; -.
DR DNASU; 5463; -.
DR GeneID; 5463; -.
DR KEGG; hsa:5463; -.
DR UCSC; uc001rxy.4; human.
DR CTD; 5463; -.
DR DisGeNET; 5463; -.
DR GeneCards; POU6F1; -.
DR HGNC; HGNC:9224; POU6F1.
DR MIM; 618043; gene.
DR neXtProt; NX_Q14863; -.
DR PharmGKB; PA33548; -.
DR eggNOG; KOG3802; Eukaryota.
DR HOGENOM; CLU_013065_6_0_1; -.
DR InParanoid; Q14863; -.
DR OrthoDB; 474850at2759; -.
DR PhylomeDB; Q14863; -.
DR TreeFam; TF350705; -.
DR PathwayCommons; Q14863; -.
DR SignaLink; Q14863; -.
DR BioGRID-ORCS; 5463; 10 hits in 1095 CRISPR screens.
DR ChiTaRS; POU6F1; human.
DR GenomeRNAi; 5463; -.
DR Pharos; Q14863; Tbio.
DR PRO; PR:Q14863; -.
DR Proteomes; UP000005640; Unplaced.
DR RNAct; Q14863; protein.
DR Genevisible; Q14863; HS.
DR GO; GO:0000785; C:chromatin; ISA:NTNU_SB.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; TAS:ProtInc.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; ISA:NTNU_SB.
DR GO; GO:0001227; F:DNA-binding transcription repressor activity, RNA polymerase II-specific; IDA:NTNU_SB.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:NTNU_SB.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; IDA:ARUK-UCL.
DR GO; GO:0007420; P:brain development; TAS:ProtInc.
DR GO; GO:0007507; P:heart development; TAS:ProtInc.
DR GO; GO:0007517; P:muscle organ development; TAS:ProtInc.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IDA:NTNU_SB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd00086; homeodomain; 1.
DR Gene3D; 1.10.260.40; -; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR InterPro; IPR013847; POU.
DR InterPro; IPR000327; POU_dom.
DR Pfam; PF00046; Homeodomain; 1.
DR Pfam; PF00157; Pou; 1.
DR PRINTS; PR00028; POUDOMAIN.
DR SMART; SM00389; HOX; 1.
DR SMART; SM00352; POU; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR SUPFAM; SSF47413; SSF47413; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
DR PROSITE; PS00035; POU_1; 1.
DR PROSITE; PS00465; POU_2; 1.
DR PROSITE; PS51179; POU_3; 1.
PE 1: Evidence at protein level;
KW 3D-structure; DNA-binding; Homeobox; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..301
FT /note="POU domain, class 6, transcription factor 1"
FT /id="PRO_0000100759"
FT DOMAIN 139..213
FT /note="POU-specific"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00530"
FT DNA_BIND 234..293
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 62..93
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 210
FT /note="N -> M (in Ref. 1; CAA79978)"
FT /evidence="ECO:0000305"
FT HELIX 144..159
FT /evidence="ECO:0007829|PDB:3D1N"
FT TURN 160..162
FT /evidence="ECO:0007829|PDB:3D1N"
FT HELIX 165..172
FT /evidence="ECO:0007829|PDB:3D1N"
FT STRAND 175..177
FT /evidence="ECO:0007829|PDB:3D1N"
FT HELIX 182..189
FT /evidence="ECO:0007829|PDB:3D1N"
FT HELIX 195..218
FT /evidence="ECO:0007829|PDB:3D1N"
FT HELIX 223..227
FT /evidence="ECO:0007829|PDB:3D1N"
FT HELIX 243..255
FT /evidence="ECO:0007829|PDB:3D1N"
FT HELIX 261..271
FT /evidence="ECO:0007829|PDB:3D1N"
FT HELIX 275..289
FT /evidence="ECO:0007829|PDB:3D1N"
SQ SEQUENCE 301 AA; 32645 MW; 9C552BA84ABAB17A CRC64;
MPGISSQILT NAQGQVIGTL PWVVNSASVA APAPAQSLQV QAVTPQLLLN AQGQVIATLA
SSPLPPPVAV RKPSTPESPA KSEVQPIQPT PTVPQPAVVI ASPAPAAKPS ASAPIPITCS
ETPTVSQLVS KPHTPSLDED GINLEEIREF AKNFKIRRLS LGLTQTQVGQ ALTATEGPAY
SQSAICRFEK LDITPKSAQK LKPVLEKWLN EAELRNQEGQ QNLMEFVGGE PSKKRKRRTS
FTPQAIEALN AYFEKNPLPT GQEITEIAKE LNYDREVVRV WFCNRRQTLK NTSKLNVFQI
P
