PO6F1_MOUSE
ID PO6F1_MOUSE Reviewed; 301 AA.
AC Q07916; Q91XK3;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 183.
DE RecName: Full=POU domain, class 6, transcription factor 1;
DE AltName: Full=Octamer-binding transcription factor EMB;
DE AltName: Full=Transcription regulatory protein MCP-1;
GN Name=Pou6f1; Synonyms=Emb;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=BALB/cJ; TISSUE=Brain, and Testis;
RX PubMed=8463278; DOI=10.1016/s0021-9258(18)53195-1;
RA Okamoto K., Wakamiya M., Noji S., Koyama E., Taniguchi S., Takemura R.,
RA Copeland N.G., Gilbert D.J., Jenkins N.A., Muramatsu M., Hamada H.;
RT "A novel class of murine POU gene predominantly expressed in central
RT nervous system.";
RL J. Biol. Chem. 268:7449-7457(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=CD-1; TISSUE=Brain;
RX PubMed=7907365; DOI=10.1523/jneurosci.14-03-01584.1994;
RA Bulleit R.F., Cui H., Wang J., Lin X.;
RT "NMDA receptor activation in differentiating cerebellar cell cultures
RT regulates the expression of a new POU gene, Cns-1.";
RL J. Neurosci. 14:1584-1595(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM C7).
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM C2).
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Transcription factor that binds preferentially to a variant
CC of the octamer motif (5'-ATGATAAT-3'). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=C2;
CC IsoId=Q07916-1; Sequence=Displayed;
CC Name=C1;
CC IsoId=Q07916-2; Sequence=VSP_002334;
CC Name=C7;
CC IsoId=Q07916-3; Sequence=VSP_002335;
CC -!- TISSUE SPECIFICITY: Isoform C1 and isoform C2 are found in the brain,
CC while isoform C7 is found in the testis.
CC -!- DEVELOPMENTAL STAGE: Expressed in the embryo throughout post-
CC implantation stages with the most prominent expression in the
CC developing CNS. In the adult, it is highly expressed in brain whereas
CC weaker expression can be detected in other organs.
CC -!- SIMILARITY: Belongs to the POU transcription factor family. Class-6
CC subfamily. {ECO:0000305}.
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DR EMBL; D13801; BAA02959.1; -; mRNA.
DR EMBL; L13763; AAA53044.1; -; mRNA.
DR EMBL; AK006732; BAB24718.1; -; mRNA.
DR EMBL; BC059830; AAH59830.1; -; mRNA.
DR PIR; B46078; B46078.
DR PIR; I56543; I56543.
DR RefSeq; NP_034257.1; NM_010127.3.
DR RefSeq; XP_011243814.1; XM_011245512.2.
DR RefSeq; XP_011243815.1; XM_011245513.2.
DR AlphaFoldDB; Q07916; -.
DR SMR; Q07916; -.
DR BioGRID; 202314; 4.
DR STRING; 10090.ENSMUSP00000073504; -.
DR PhosphoSitePlus; Q07916; -.
DR PaxDb; Q07916; -.
DR PRIDE; Q07916; -.
DR ProteomicsDB; 289713; -. [Q07916-1]
DR ProteomicsDB; 289714; -. [Q07916-2]
DR ProteomicsDB; 289715; -. [Q07916-3]
DR Antibodypedia; 14350; 253 antibodies from 28 providers.
DR Ensembl; ENSMUST00000176271; ENSMUSP00000145707; ENSMUSG00000009739. [Q07916-1]
DR UCSC; uc007xro.1; mouse. [Q07916-3]
DR UCSC; uc007xrp.1; mouse. [Q07916-1]
DR MGI; MGI:102935; Pou6f1.
DR VEuPathDB; HostDB:ENSMUSG00000009739; -.
DR eggNOG; KOG3802; Eukaryota.
DR GeneTree; ENSGT00940000160106; -.
DR HOGENOM; CLU_013065_6_0_1; -.
DR InParanoid; Q07916; -.
DR OrthoDB; 474850at2759; -.
DR PhylomeDB; Q07916; -.
DR BioGRID-ORCS; 19009; 0 hits in 75 CRISPR screens.
DR ChiTaRS; Pou6f1; mouse.
DR PRO; PR:Q07916; -.
DR Proteomes; UP000000589; Chromosome 15.
DR RNAct; Q07916; protein.
DR Bgee; ENSMUSG00000009739; Expressed in retinal neural layer and 238 other tissues.
DR ExpressionAtlas; Q07916; baseline and differential.
DR Genevisible; Q07916; MM.
DR GO; GO:0005634; C:nucleus; IDA:MGI.
DR GO; GO:0003677; F:DNA binding; IDA:MGI.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0001227; F:DNA-binding transcription repressor activity, RNA polymerase II-specific; ISO:MGI.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:MGI.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:MGI.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; ISO:MGI.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd00086; homeodomain; 1.
DR Gene3D; 1.10.260.40; -; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR InterPro; IPR013847; POU.
DR InterPro; IPR000327; POU_dom.
DR Pfam; PF00046; Homeodomain; 1.
DR Pfam; PF00157; Pou; 1.
DR PRINTS; PR00028; POUDOMAIN.
DR SMART; SM00389; HOX; 1.
DR SMART; SM00352; POU; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR SUPFAM; SSF47413; SSF47413; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
DR PROSITE; PS00035; POU_1; 1.
DR PROSITE; PS00465; POU_2; 1.
DR PROSITE; PS51179; POU_3; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; DNA-binding; Homeobox; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..301
FT /note="POU domain, class 6, transcription factor 1"
FT /id="PRO_0000100760"
FT DOMAIN 139..213
FT /note="POU-specific"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00530"
FT DNA_BIND 234..293
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 65..88
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..131
FT /note="MPGISSQILTNAQGQVIGALPWVVNSASVATPAPAQSLQVQAVTPQLLLNAQ
FT GQVIATLASSPLPQPVAVRKPNTPESPAKSEVQPIQPTQAVPQPAVILTSPTPALKPSA
FT ATPIPITCSETPTVSQLVSK -> MCQHTLIFMHVSLVPPSGLE (in isoform
FT C7)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_002335"
FT VAR_SEQ 131
FT /note="K -> SLE (in isoform C1)"
FT /evidence="ECO:0000305"
FT /id="VSP_002334"
SQ SEQUENCE 301 AA; 32852 MW; 9FC71EE845982AD4 CRC64;
MPGISSQILT NAQGQVIGAL PWVVNSASVA TPAPAQSLQV QAVTPQLLLN AQGQVIATLA
SSPLPQPVAV RKPNTPESPA KSEVQPIQPT QAVPQPAVIL TSPTPALKPS AATPIPITCS
ETPTVSQLVS KPHTPSLDED GINLEEIREF AKNFKIRRLS LGLTQTQVGQ ALTATEGPAY
SQSAICRFEK LDITPKSAQK LKPVLEKWLM EAELRNQEGQ QNLMEFVGGE PSKKRKRRTS
FTPQAIEALN AYFEKNPLPT GQEITEIAKE LNYDREVVRV WFCNRRQTLK NTSKLNVFQI
P
