ID   PO6F1_RAT               Reviewed;         301 AA.
AC   P56223;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   15-JUL-1998, sequence version 1.
DT   03-AUG-2022, entry version 157.
DE   RecName: Full=POU domain, class 6, transcription factor 1;
DE   AltName: Full=Brain-specific homeobox/POU domain protein 5;
DE            Short=Brain-5;
DE            Short=Brn-5;
GN   Name=Pou6f1; Synonyms=Brn5;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=Fischer; TISSUE=Pituitary anterior lobe;
RX   PubMed=7901208; DOI=10.1016/s0021-9258(19)49475-1;
RA   Andersen B., Schonemann M.D., Pearse R.V. II, Jenne K., Sugarman J.,
RA   Rosenfeld M.G.;
RT   "Brn-5 is a divergent POU domain factor highly expressed in layer IV of the
RT   neocortex.";
RL   J. Biol. Chem. 268:23390-23398(1993).
CC   -!- FUNCTION: Transcription factor that binds preferentially to a variant
CC       of the octamer motif (5'-ATGATAAT-3').
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   -!- TISSUE SPECIFICITY: In the embryo, widely expressed, with highest
CC       levels in the developing brain and spinal cord. In the adult, mostly
CC       found in the brain, where it is diffusely expressed with the exception
CC       of an enrichment in layer IV of the neocortex. Also found in kidney,
CC       lung, heart, adrenal, skin, and placenta. Low levels in spleen, muscle,
CC       liver, anterior pituitary, testis and ovary.
CC   -!- DOMAIN: Contains two direct repeats of 7 amino acids in the N-terminus.
CC   -!- SIMILARITY: Belongs to the POU transcription factor family. Class-6
CC       subfamily. {ECO:0000305}.
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DR   EMBL; L23204; -; NOT_ANNOTATED_CDS; mRNA.
DR   PIR; A48880; A48880.
DR   RefSeq; NP_001099216.1; NM_001105746.1.
DR   RefSeq; XP_017450058.1; XM_017594569.1.
DR   RefSeq; XP_017450059.1; XM_017594570.1.
DR   RefSeq; XP_017450060.1; XM_017594571.1.
DR   AlphaFoldDB; P56223; -.
DR   SMR; P56223; -.
DR   STRING; 10116.ENSRNOP00000006095; -.
DR   PaxDb; P56223; -.
DR   Ensembl; ENSRNOT00000006095; ENSRNOP00000006095; ENSRNOG00000004595.
DR   GeneID; 116545; -.
DR   KEGG; rno:116545; -.
DR   UCSC; RGD:620615; rat.
DR   CTD; 5463; -.
DR   RGD; 620615; Pou6f1.
DR   eggNOG; KOG3802; Eukaryota.
DR   GeneTree; ENSGT00940000160106; -.
DR   HOGENOM; CLU_013065_6_0_1; -.
DR   InParanoid; P56223; -.
DR   OMA; SEMQPIQ; -.
DR   OrthoDB; 474850at2759; -.
DR   PhylomeDB; P56223; -.
DR   PRO; PR:P56223; -.
DR   Proteomes; UP000002494; Chromosome 7.
DR   Bgee; ENSRNOG00000004595; Expressed in frontal cortex and 18 other tissues.
DR   Genevisible; P56223; RN.
DR   GO; GO:0005634; C:nucleus; ISO:RGD.
DR   GO; GO:0003677; F:DNA binding; IDA:RGD.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR   GO; GO:0001227; F:DNA-binding transcription repressor activity, RNA polymerase II-specific; ISO:RGD.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; ISO:RGD.
DR   GO; GO:0043565; F:sequence-specific DNA binding; ISO:RGD.
DR   GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:RGD.
DR   GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; ISO:RGD.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   CDD; cd00086; homeodomain; 1.
DR   Gene3D; 1.10.260.40; -; 1.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR001356; Homeobox_dom.
DR   InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR   InterPro; IPR013847; POU.
DR   InterPro; IPR000327; POU_dom.
DR   Pfam; PF00046; Homeodomain; 1.
DR   Pfam; PF00157; Pou; 1.
DR   PRINTS; PR00028; POUDOMAIN.
DR   SMART; SM00389; HOX; 1.
DR   SMART; SM00352; POU; 1.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   SUPFAM; SSF47413; SSF47413; 1.
DR   PROSITE; PS50071; HOMEOBOX_2; 1.
DR   PROSITE; PS00035; POU_1; 1.
DR   PROSITE; PS00465; POU_2; 1.
DR   PROSITE; PS51179; POU_3; 1.
PE   2: Evidence at transcript level;
KW   DNA-binding; Homeobox; Nucleus; Reference proteome; Repeat; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..301
FT                   /note="POU domain, class 6, transcription factor 1"
FT                   /id="PRO_0000100761"
FT   REPEAT          11..17
FT                   /note="1"
FT   REPEAT          50..56
FT                   /note="2"
FT   DOMAIN          139..213
FT                   /note="POU-specific"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00530"
FT   DNA_BIND        234..293
FT                   /note="Homeobox"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT   REGION          11..56
FT                   /note="2 X 7 AA repeats of N-A-Q-G-Q-V-I"
FT   REGION          66..88
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   301 AA;  32702 MW;  44A50F627FEB9F28 CRC64;
     MPGISSPILT NAQGQVIGAL PWVVNSASVA TPAPAQSLQV QAVTPQLLLN AQGQVIATLA
     SSPLPQPVAV RKPSTPESPA KSEVQPIQPT QAVPPPAVIL TSPAPALKPS ASAPIPITCS
     ETPTVSQLVS KPHTPSLDED GINLEEIREF AKNFKIRRLS LGLTQTQVGQ ALTATEGPAY
     SQSAICRFEK LDITPKSAQK LKPVLEKWLN EAELRNQEGQ QNLMEFVGGE PSKKRKRRTS
     FTPQAIEALN AYFEKNPLPT GQEITEIAKE LNYDREVVRV WFCNRRQTLK NTSKLNVFQI
     P