ID   POBA_PSEOL              Reviewed;         409 AA.
AC   Q52185;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 76.
DE   RecName: Full=Phenoxybenzoate dioxygenase subunit alpha;
DE            EC=1.14.12.-;
DE   AltName: Full=4-carboxydiphenyl ether;phenoxybenzoate dioxygenase;
GN   Name=pobA;
OS   Pseudomonas oleovorans.
OG   Plasmid pPOB.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas;
OC   Pseudomonas oleovorans/pseudoalcaligenes group.
OX   NCBI_TaxID=301;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=POB310;
RX   PubMed=7710319; DOI=10.1007/bf00262201;
RA   Dehmel U., Engesser K.-H., Timmis K.N., Dwyer D.F.;
RT   "Cloning, nucleotide sequence, and expression of the gene encoding a novel
RT   dioxygenase involved in metabolism of carboxydiphenyl ethers in Pseudomonas
RT   pseudoalcaligenes POB310.";
RL   Arch. Microbiol. 163:35-41(1995).
CC   -!- FUNCTION: Degrades exclusively diarylether compounds having carboxyl
CC       groups in the 3- or 4-position. Yields a hemiacetal that spontaneously
CC       hydrolyzes to phenol and protocatechuate.
CC   -!- COFACTOR:
CC       Name=[2Fe-2S] cluster; Xref=ChEBI:CHEBI:190135; Evidence={ECO:0000305};
CC       Note=Binds 1 [2Fe-2S] cluster. {ECO:0000305};
CC   -!- COFACTOR:
CC       Name=Fe cation; Xref=ChEBI:CHEBI:24875; Evidence={ECO:0000305};
CC       Note=Binds 1 Fe cation. {ECO:0000305};
CC   -!- PATHWAY: Aromatic compound metabolism; carboxydiphenyl ether
CC       degradation.
CC   -!- SUBUNIT: This dioxygenase system consists of two proteins: the alpha
CC       subunit (PobA) and a subunit (PobB) that acts as a ferredoxin and a
CC       ferredoxin reductase.
CC   -!- INDUCTION: By 3- or 4-carboxydiphenyl ether and by phenol.
CC   -!- SIMILARITY: Belongs to the bacterial ring-hydroxylating dioxygenase
CC       alpha subunit family. {ECO:0000305}.
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DR   EMBL; X78823; CAA55400.1; -; Genomic_DNA.
DR   PIR; S44171; S44171.
DR   AlphaFoldDB; Q52185; -.
DR   SMR; Q52185; -.
DR   UniPathway; UPA00730; -.
DR   GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0051213; F:dioxygenase activity; IEA:UniProtKB-KW.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0019439; P:aromatic compound catabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 2.102.10.10; -; 1.
DR   InterPro; IPR017941; Rieske_2Fe-2S.
DR   InterPro; IPR036922; Rieske_2Fe-2S_sf.
DR   InterPro; IPR015881; Ring-hydroxy_dOase_2Fe2S_BS.
DR   Pfam; PF00355; Rieske; 1.
DR   SUPFAM; SSF50022; SSF50022; 1.
DR   PROSITE; PS51296; RIESKE; 1.
DR   PROSITE; PS00570; RING_HYDROXYL_ALPHA; 1.
PE   2: Evidence at transcript level;
KW   2Fe-2S; Aromatic hydrocarbons catabolism; Dioxygenase; Iron; Iron-sulfur;
KW   Metal-binding; NAD; Oxidoreductase; Plasmid.
FT   CHAIN           1..409
FT                   /note="Phenoxybenzoate dioxygenase subunit alpha"
FT                   /id="PRO_0000085057"
FT   DOMAIN          45..149
FT                   /note="Rieske"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00628"
FT   BINDING         85
FT                   /ligand="[2Fe-2S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:190135"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00628"
FT   BINDING         87
FT                   /ligand="[2Fe-2S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:190135"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00628"
FT   BINDING         104
FT                   /ligand="[2Fe-2S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:190135"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00628"
FT   BINDING         107
FT                   /ligand="[2Fe-2S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:190135"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00628"
FT   BINDING         210
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /evidence="ECO:0000250"
FT   BINDING         215
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   409 AA;  46259 MW;  721B634A8FA7FD2F CRC64;
     MSKTIPIVDA QHAGSAYQHV PGHPDPQLSA VAKGTPTGEY LRRYWQPVAL SADVTDRPQM
     VRILGEDLVL FRDKAGRPGL LYPRCMHRGT SLYYGHVEEA GIRCCYHGWL FAVDGTCLNQ
     PCEPEGGLRR EAARQPWYPV EERYGLVFAY MGPPEKKPVL PRYDILEDLE EGEFIEVISG
     GFVSYADHVE DPNVPYHWLQ NWENIMDPYH VYILHSTFSG IQFAENFKIL PRVDFEAVDG
     GVIYHAWRDL EDGRQLERIN SALFPNISAI PMIDLSPGQG RWIGWHVAVD DQHYRGFFAA
     RTRQPGNFAP IKMHNGKSWT ELSEQEKQDF PGDFEAQFGQ GRVTLHGEEH LATSDHGIAL
     LRRQMKQQIA IVQQGGDPAG VHFNEADALV RIRSGNFYTT SDKTETAAD