POC1_YEAST
ID POC1_YEAST Reviewed; 276 AA.
AC Q05778; D6VYK1;
DT 20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 20-DEC-2005, sequence version 2.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=Proteasome chaperone 1;
DE AltName: Full=Proteasome biogenesis-associated protein 1;
GN Name=PBA1; Synonyms=POC1; OrderedLocusNames=YLR199C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1] {ECO:0000312|EMBL:AAB67437.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169871;
RA Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W.,
RA Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A.,
RA Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K.,
RA Heuss-Neitzel D., Hilbert H., Hilger F., Kleine K., Koetter P., Louis E.J.,
RA Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S.,
RA Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D.,
RA Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M.,
RA Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P.,
RA Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M.,
RA Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K.,
RA Zollner A., Hani J., Hoheisel J.D.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.";
RL Nature 387:87-90(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP PROTEIN SEQUENCE OF 5-23; 35-75; 92-108 AND 110-174, GENE NAME, INTERACTION
RP WITH ADD66, SUBUNIT, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=17431397; DOI=10.1038/sj.emboj.7601681;
RA Li X., Kusmierczyk A.R., Wong P., Emili A., Hochstrasser M.;
RT "Beta-subunit appendages promote 20S proteasome assembly by overcoming an
RT Ump1-dependent checkpoint.";
RL EMBO J. 26:2339-2349(2007).
RN [4] {ECO:0000305}
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [5] {ECO:0000305}
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [6] {ECO:0000305}
RP REVISION OF GENE MODEL.
RX PubMed=12775844; DOI=10.1126/science.1084337;
RA Cliften P.F., Sudarsanam P., Desikan A., Fulton L., Fulton B., Majors J.,
RA Waterston R., Cohen B.A., Johnston M.;
RT "Finding functional features in Saccharomyces genomes by phylogenetic
RT footprinting.";
RL Science 301:71-76(2003).
RN [7]
RP GENE NAME, FUNCTION, INTERACTION WITH ADD66, AND SUBUNIT.
RX PubMed=17707236; DOI=10.1016/j.molcel.2007.06.025;
RA Le Tallec B., Barrault M.-B., Courbeyrette R., Guerois R.,
RA Marsolier-Kergoat M.-C., Peyroche A.;
RT "20S proteasome assembly is orchestrated by two distinct pairs of
RT chaperones in yeast and in mammals.";
RL Mol. Cell 27:660-674(2007).
CC -!- FUNCTION: Involved in 20S proteasome assembly.
CC {ECO:0000269|PubMed:17707236}.
CC -!- SUBUNIT: Component of the 20S proteasome chaperone. Forms a heterodimer
CC with ADD66 that binds to proteasome precursors.
CC {ECO:0000269|PubMed:17431397, ECO:0000269|PubMed:17707236}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:14562095}.
CC -!- MISCELLANEOUS: Present with 1350 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the PSMG1 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB67437.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; U14913; AAB67437.1; ALT_SEQ; Genomic_DNA.
DR EMBL; BK006945; DAA09517.1; -; Genomic_DNA.
DR PIR; S48551; S48551.
DR RefSeq; NP_013300.2; NM_001182086.1.
DR PDB; 4G4S; X-ray; 2.49 A; O=1-276.
DR PDB; 7LS6; EM; 3.17 A; O=1-276.
DR PDB; 7LSX; EM; 3.61 A; O=1-276.
DR PDBsum; 4G4S; -.
DR PDBsum; 7LS6; -.
DR PDBsum; 7LSX; -.
DR AlphaFoldDB; Q05778; -.
DR SMR; Q05778; -.
DR BioGRID; 31468; 101.
DR DIP; DIP-4796N; -.
DR IntAct; Q05778; 3.
DR MINT; Q05778; -.
DR STRING; 4932.YLR199C; -.
DR iPTMnet; Q05778; -.
DR MaxQB; Q05778; -.
DR PaxDb; Q05778; -.
DR PRIDE; Q05778; -.
DR EnsemblFungi; YLR199C_mRNA; YLR199C; YLR199C.
DR GeneID; 850896; -.
DR KEGG; sce:YLR199C; -.
DR SGD; S000004189; PBA1.
DR VEuPathDB; FungiDB:YLR199C; -.
DR eggNOG; ENOG502RY9B; Eukaryota.
DR HOGENOM; CLU_095376_0_0_1; -.
DR InParanoid; Q05778; -.
DR OMA; MLFKQWN; -.
DR BioCyc; YEAST:G3O-32319-MON; -.
DR PRO; PR:Q05778; -.
DR Proteomes; UP000002311; Chromosome XII.
DR RNAct; Q05778; protein.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR GO; GO:0043248; P:proteasome assembly; IGI:SGD.
DR Gene3D; 3.40.50.12120; -; 2.
DR InterPro; IPR038605; Pba1_sf.
DR InterPro; IPR018855; Psome_chaperone_1_fun.
DR Pfam; PF10450; POC1; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Chaperone; Cytoplasm; Direct protein sequencing;
KW Reference proteome.
FT CHAIN 1..276
FT /note="Proteasome chaperone 1"
FT /id="PRO_0000203234"
FT STRAND 35..37
FT /evidence="ECO:0007829|PDB:4G4S"
FT HELIX 44..46
FT /evidence="ECO:0007829|PDB:7LS6"
FT STRAND 49..54
FT /evidence="ECO:0007829|PDB:4G4S"
FT HELIX 55..60
FT /evidence="ECO:0007829|PDB:4G4S"
FT TURN 63..67
FT /evidence="ECO:0007829|PDB:4G4S"
FT STRAND 69..71
FT /evidence="ECO:0007829|PDB:4G4S"
FT STRAND 74..76
FT /evidence="ECO:0007829|PDB:4G4S"
FT STRAND 124..135
FT /evidence="ECO:0007829|PDB:4G4S"
FT TURN 139..141
FT /evidence="ECO:0007829|PDB:4G4S"
FT HELIX 144..159
FT /evidence="ECO:0007829|PDB:4G4S"
FT STRAND 164..171
FT /evidence="ECO:0007829|PDB:4G4S"
FT STRAND 178..183
FT /evidence="ECO:0007829|PDB:4G4S"
FT HELIX 196..205
FT /evidence="ECO:0007829|PDB:4G4S"
FT TURN 208..211
FT /evidence="ECO:0007829|PDB:4G4S"
FT STRAND 214..220
FT /evidence="ECO:0007829|PDB:4G4S"
FT STRAND 222..224
FT /evidence="ECO:0007829|PDB:4G4S"
FT HELIX 232..246
FT /evidence="ECO:0007829|PDB:4G4S"
FT HELIX 252..263
FT /evidence="ECO:0007829|PDB:4G4S"
FT HELIX 265..267
FT /evidence="ECO:0007829|PDB:4G4S"
FT HELIX 268..271
FT /evidence="ECO:0007829|PDB:4G4S"
FT STRAND 274..276
FT /evidence="ECO:0007829|PDB:4G4S"
SQ SEQUENCE 276 AA; 30695 MW; 783C930795771748 CRC64;
MLFKQWNDLP EPKHLLDLPE ISKNLQSLEV CPVPKVEFPQ DLDVPQYSTA VITTKIMNPL
FPKNLLQLTS IGEIKTTLTV KSPSLPQSSG KHSWNYDENF PNEVDPDQKN DTADETVYGF
SFPIYSFGKT LLFSMEENFI SISPIFGNMI SRSIISQLAQ FSPDIIVIGT SDKIASMKVM
TENECTLQPP EFITGFIGSV LTQLIVGPSK GLKFKCLVAP SEGPNGFEKL SLSDMGSLVD
LCGQWLGFEP SRYSEECYRL WRCDSAAIGA QSGLYI
